Recombinant Chicken Hypoxia-inducible factor 1-alpha(HIF1A) ,partial

Code CSB-YP896655CH
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Source Yeast
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Code CSB-EP896655CH
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Source E.coli
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Code CSB-EP896655CH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896655CH
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Source Baculovirus
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Code CSB-MP896655CH
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names HIF1A
Uniprot No. Q9YIB9
Alternative Names HIF1AHypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha
Species Gallus gallus (Chicken)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators (By similarity).
Gene References into Functions
  1. HIF-1alpha, -2alpha, and -3alpha expressions in the tibial growth plate of Tibetan chickens were upregulated by hypoxia and the change was more significant than in Arbor Acres chickens. PMID: 28693310
  2. FK228 (HIF-1alpha inhibitor) significantly (P less than 0.05) down- regulated the mRNA and protein levels of HIF-1alpha and Hsp90, restored the size of growth plate and diminished lameness. In conclusion, HIF-1alpha and Hsp90 play an important role in the formation of avascular growth plate and there is a direct relationship between HIF-1alpha and Hsp90 for the progression of TD pathogenesis PMID: 29504370
  3. Altering HIF-1alpha through 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure affects coronary vessel development. PMID: 23264063
  4. These findings hinted that HIF-1alpha might be associated with the development of ascites syndrome induced by excess salt in drinking water in broiler chickens. PMID: 23873551
  5. Epicardial HIF signaling regulates vascular precursor cell invasion into the myocardium PMID: 23384563
  6. HIF-1alpha induction during hypoxic preconditioning is necessary to obtain enhanced HIF-1alpha accumulation and to develop tolerance against a subsequent hypoxic injury. PMID: 21953610
  7. Data suggest that developing coronary vessels may be subject to a level of regulation that is dependent on differential oxygen levels within cardiac tissues and subsequent HIF-1alpha regulation of gene expression. PMID: 19777592
  8. Results showed that HIF-1alpha mRNA displayed tissue specific differential expression, with the highest in the brain PMID: 17284428
  9. This study found that tissue hypoxia is likely a normal component of embryonic development in the chicken based on the upregulation of hypoxia response genes. PMID: 17954589
  10. Hypoxia signaling through HIF-1 and VEGF provides an autocrine survival signal in the developing cardiac output flow tract[OFT] and that perturbation in this pathway causes OFT defects that model congenital human conotruncal heart defects. PMID: 18467628

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Subcellular Location Cytoplasm, Nucleus, Nucleus speckle
Database Links

KEGG: gga:374177

STRING: 9031.ENSGALP00000036587

UniGene: Gga.4933

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