Recombinant Dog Angiopoietin-2(ANGPT2)

Code CSB-YP001707DO
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Source Yeast
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Code CSB-EP001707DO
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Source E.coli
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Code CSB-EP001707DO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001707DO
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Source Baculovirus
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Code CSB-MP001707DO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ANGPT2
Uniprot No. A0A8J8
Alternative Names ANGPT2Angiopoietin-2; ANG-2
Species Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region 19-495
Target Protein Sequence YN NFRRSMDSIG RRQYQVQHGS CSYTFLLPET DNCRSPGSYV PNAVQRDAPL DYDDSVQRLQ VLENIMENNT QWLIKLENYI QDNMKKEMVE MQQNAVQNQT AVMIEIGTNL LNQTAEQTRK LTDVEAQVLN QTTRLELQLL EHSLSTNKLE KQILDQTSEI NKLQDKNSFL EKKVLDMEDK HIVQLRSIKE EKDQLQVLVS KQNSIIEELE KQLVTATVNN SVLQKQQHDL METVHSLLTM ISPSKSPKDT FVAKEEQIIY RDCAEVFKSG LTTNGIYTLT FPNSTEEIKA YCDMETSGGG WTVIQRREDG SVDFQRTWKE YKVGFGNPSG EHWLGNEFVF QVTNQQPYVL KIHLKDWEGN EAYSLYEHFY LSGEELNYRI HLKGLTGTAG KISSISQPGN DFSTKDADND KCICKCSQML TGGWWFDACG PSNLNGMYYP QRQNTNKFNG IKWYYWKGSG YSLKGTTMMI RPADF
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Subcellular Location Secreted.
Database Links

KEGG: cfa:607616

STRING: 9615.ENSCAFP00000012670

UniGene: Cfa.13296

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