Recombinant Dog Angiopoietin-2 (ANGPT2) (Active)

This Recombinant Dog Angiopoietin-2 (ANGPT2) was produced in Mammalian cell, where the gene sequence encoding Dog ANGPT2 (19-495aa) was expressed with the C-terminal 6xHis tag. The purity of this ANGPT2 protein was greater than 95% by SDS-PAGE. The activity was validated by its binding ability in a functional ELISA.

The similarity between dog ANGPT2 and human ANGPT2 is very high, reaching 92.13%. The dog ANGPT2 gene encodes 495 amino acids, the first 18 amino acids are signal peptides, and the mature protein has 477 amino acids. ANGPT2 is mainly secreted by endothelial cells. It is a natural competitive antagonist of ANGPT1. It can competitively inhibit the phosphorylation of ANGPT1 to promote TIE2. ANGPT2 is mainly expressed in vascular endothelial cells and can maintain the dynamic of vascular growth and degeneration by inhibiting the activation of ANGPT1 balance. In addition, the expression of ANGPT2 is regulated by a variety of factors such as VEGF, EGF, bFGF, TNFα, etc. Hypoxia can promote the expression of ANGPT2, However, ANGPT1 and TGF-β down-regulated their expression levels. The effect of ANGPT2 on angiogenesis is related to the local microenvironment. In the presence of VEGF, ANGPT2 can antagonize the effect of ANGPT1 on vascular structure stabilization, which is beneficial to vascular sprouting and growth. When VEGF is lacking, ANGPT2 inhibits ANGPT1, which is beneficial to vascular regression.