Recombinant Drosophila melanogaster Death domain-containing adapter protein BG4 (BG4)

Code CSB-YP893221DLU
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Source Yeast
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Code CSB-EP893221DLU
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Source E.coli
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Code CSB-EP893221DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893221DLU
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Source Baculovirus
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Code CSB-MP893221DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fadd
Uniprot No.
Alternative Names
Fadd; BG4; CG12297Fas-associated death domain protein; Death domain-containing adapter protein BG4; FAS-associating death domain-containing protein; dFADD
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-239
Target Protein Sequence
MTAGRHWSYD SLKQIAIDGC TENVEQLKLI FVEEIGSRRR SDCIRTIEDL IDCLERADEL SEYNVEPLRR ISGNMPQLIE ALSAYTKPEN ILGHPVNLYQ ELRLAEELRQ QLRIAPASQN AQPSVSELAA AVPPTAIQNY ATPAAFTDHK RTMVFKKISE ELGRYWRRLG RSAGIGEGQM DTIEERYPHD LKSQILRLLQ LIEEDDCHDP KHFLLRLCRA LGDCGRNDLR KRVEQIMSH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the IMD signaling pathway and is required for the host defense against Gram-negative bacteria. Interacts with Dredd, promotes cleavage of Dredd and is necessary and sufficient for enhancing Dredd-induced apoptosis.
Gene References into Functions
  1. demonstrate that dFADD is a novel component of the immune deficiency pathway; double-stranded RNA expression reduces the induction of antibacterial peptide-encoding genes after infection and renders the fly susceptible to Gram-negative bacterial infection PMID: 12123572
  2. dFADD acts downstream of IMD protein in the pathway that controls inducibility of the antibacterial peptide genes PMID: 12433364
Subcellular Location
Cytoplasm.
Database Links

KEGG: dme:Dmel_CG12297

STRING: 7227.FBpp0083574

UniGene: Dm.1273

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