FADD

FADD, also known as Fas-associated death domain protein, is a crucial adaptor molecule. It is also commonly referred to as MORT1. FADD plays a key role in apoptotic signaling. It binds to the death domain of Fas receptor and recruits caspase - 8, initiating the caspase cascade and leading to cell apoptosis. This process is mainly involved in the extrinsic apoptotic pathway.
Dysregulation of FADD has been linked to various diseases like cancer and autoimmune disorders. In the field of drug development, targeting FADD - related pathways is a promising area. Some research is exploring small - molecule inhibitors that can modulate FADD - mediated apoptosis, aiming to develop new therapies for relevant diseases.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

FADD Recombinant Monoclonal Antibody (CSB-RA158850A0HU)

Validated Data

Western Blot
Positive WB detected in: A549 whole cell lysate(20µg),Hela whole cell lysate(20µg), Jurkat whole cell lysate(20µg),NIH/3T3 whole cell lysate(20µg)
All lanes: FADD antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time：2min

Overlay Peak curve showing HepG2 cells stained with CSB-RA158850A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

FADD Antibody (CSB-PA824751)

Validated Data

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA824751(FADD Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA824751(FADD Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 8+12%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Mouse spleen tissue, RAW264.7 cells, Primary antibody: CSB-PA824751(FADD Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

Human Protein FADD(FADD) ELISA kit (CSB-EL007958HU)

Validated Data

Code: CSB-EL007958HU

Size: 96T,5×96T,10×96T

Sensitivity: 31.25 pg/mL

Detection Range: 125 pg/mL-8000 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following FADD reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

FADD Antibodies

FADD Proteins

FADD ELISA Kits

FADD Antibodies

FADD Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA007958GA01HU	FADD Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA623788ESR1HU	FADD Antibody	Human	ELISA, IHC
CSB-PA446731	Phospho-FADD (Ser191) Antibody	Human,Mouse	ELISA,WB,IHC
CSB-PA002463	FADD Antibody	Human	WB, IHC, ELISA
CSB-PA070144	Phospho-FADD (S194) Antibody	Human	WB, IHC, ELISA
CSB-PA070145	FADD Antibody	Human	IHC, IF, ELISA
CSB-PA824751	FADD Antibody	Human,Mouse	ELISA,WB,IHC
CSB-PA221364	FADD Antibody	Human	ELISA,IHC
CSB-RA158850A0HU	FADD Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, FC

FADD Antibodies for Mus musculus (Mouse)

Code	Product Name	Species Reactivity	Application
CSB-PA975869	FADD (Ab-191) Antibody	Human,Mouse	ELISA,WB
CSB-PA006346	Phospho-FADD (S191) Antibody	Mouse	WB, IHC, ELISA
CSB-PA011057	FADD Antibody	Mouse	WB, IHC, ELISA

FADD Antibodies for Drosophila melanogaster (Fruit fly)

Code	Product Name	Species Reactivity	Application
CSB-PA893221XA01DLU	Fadd Antibody	Drosophila melanogaster (Fruit fly)	ELISA, WB (ensure identification of antigen)

FADD Antibodies for Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

Code	Product Name	Species Reactivity	Application
CSB-PA302467XA01FQR	fadD Antibody	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	ELISA, WB (ensure identification of antigen)

FADD Antibodies for Escherichia coli (strain K12)

Code	Product Name	Species Reactivity	Application
CSB-PA300208XA01ENV	fadD Antibody	Escherichia coli (strain K12)	ELISA, WB (ensure identification of antigen)
CSB-PA300208ZA01ENV	fadD Antibody	Escherichia coli	ELISA, WB (ensure identification of antigen)

FADD Antibodies for Escherichia coli O157:H7

Code	Product Name	Species Reactivity	Application
CSB-PA834851XA01EOD	fadD Antibody	Escherichia coli O157:H7	ELISA, WB (ensure identification of antigen)

FADD Proteins

FADD Proteins for Escherichia coli (strain K12)

Code	Product Name	Source
CSB-YP300208ENV CSB-BP300208ENV CSB-MP300208ENV CSB-EP300208ENV-B	Recombinant Escherichia coli Long-chain-fatty-acid--CoA ligase (fadD)	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP300208ENV	Recombinant Escherichia coli Long-chain-fatty-acid--CoA ligase (fadD)	E.coli

FADD Proteins for Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

Code	Product Name	Source
CSB-YP302467EGX CSB-EP302467EGX CSB-BP302467EGX CSB-MP302467EGX CSB-EP302467EGX-B	Recombinant Long-chain-fatty-acid--CoA ligase (fadD)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Code	Product Name	Source
CSB-YP342804HTA CSB-EP342804HTA CSB-BP342804HTA CSB-MP342804HTA CSB-EP342804HTA-B	Recombinant Haemophilus influenzae Long-chain-fatty-acid--CoA ligase (fadD), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Code	Product Name	Source
CSB-YP351269SXB CSB-EP351269SXB CSB-BP351269SXB CSB-MP351269SXB CSB-EP351269SXB-B	Recombinant Salmonella typhimurium Long-chain-fatty-acid--CoA ligase (fadD), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Salmonella typhi

Code	Product Name	Source
CSB-YP351875SWW CSB-EP351875SWW CSB-BP351875SWW CSB-MP351875SWW CSB-EP351875SWW-B	Recombinant Long-chain-fatty-acid--CoA ligase (fadD), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP623788HU CSB-BP623788HU CSB-MP623788HU CSB-EP623788HU-B	Recombinant Human FAS-associated death domain protein (FADD)	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP623788HU	Recombinant Human FAS-associated death domain protein (FADD)	E.coli

FADD Proteins for Drosophila pseudoobscura pseudoobscura (Fruit fly)

Code	Product Name	Source
CSB-YP639067DME CSB-EP639067DME CSB-BP639067DME CSB-MP639067DME CSB-EP639067DME-B	Recombinant Drosophila pseudoobscura pseudoobscura Death domain-containing adapter protein BG4 (BG4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP730727MO CSB-EP730727MO CSB-BP730727MO CSB-MP730727MO CSB-EP730727MO-B	Recombinant Mouse Protein FADD (Fadd)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP737367BO CSB-EP737367BO CSB-BP737367BO CSB-MP737367BO CSB-EP737367BO-B	Recombinant Bovine Protein FADD (FADD)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Escherichia coli O157:H7

Code	Product Name	Source
CSB-YP834851EOD CSB-EP834851EOD CSB-BP834851EOD CSB-MP834851EOD CSB-EP834851EOD-B	Recombinant Escherichia coli O157:H7 Long-chain-fatty-acid--CoA ligase (fadD)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Yersinia pestis

Code	Product Name	Source
CSB-YP835296YAS CSB-EP835296YAS CSB-BP835296YAS CSB-MP835296YAS CSB-EP835296YAS-B	Recombinant Long-chain-fatty-acid--CoA ligase (fadD), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD Proteins for Drosophila melanogaster (Fruit fly)

Code	Product Name	Source
CSB-YP893221DLU CSB-EP893221DLU CSB-BP893221DLU CSB-MP893221DLU CSB-EP893221DLU-B	Recombinant Drosophila melanogaster Death domain-containing adapter protein BG4 (BG4)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

FADD ELISA Kit

FADD ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL007958HU	Human Protein FADD(FADD) ELISA kit	serum, plasma, cell culture supernates, cell lysates	31.25 pg/mL

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FADD

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