Recombinant Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3)

1. PI3K-Akt pathway inhibitors, Akti-1/2 and LY294002, reduced PFKFB3 gene induction by PHA, as well as Fru-2,6-P2 and lactate production. Moreover, both inhibitors blocked activation and proliferation in response to PHA, showing the importance of PI3K/Akt signaling pathway in the antigen response of T-lymphocytes. PMID: 29435871
2. conclude that PFKFB3 bears an oncogene-like regulatory element in lung adenocarcinoma progression PMID: 29327288
3. Results indicate that PFKFB3 is involved in the proliferation, migration, invasion and angiogenesis of breast cancer. PMID: 29393396
4. data suggesting that p53 promotes nucleotide biosynthesis in response to DNA damage by repressing the expression of the phosphofructokinase-2 (PFK2) isoform 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), a rate-limiting enzyme that promotes glycolysis. PMID: 27901115
5. Study provides evidence that PFKFB3 overexpression occupied a dominant position in sorafenib resistance of hepatocellular carcinoma cells. PMID: 28857200
6. This work has uncovered a novel function of the enzymes PFKFB3 and PFKFB4 in ovarian cancer cells during mitotic arrest PMID: 28152500
7. PFK-2 seems to be a strategic element that links NADPH oxidase activation and glycolysis modulation, and, as such, is proposed as a potential therapeutic target in inflammatory diseases. PMID: 27799347
8. Data suggest that hepatic glucokinase activity is regulated by reversible binding to specific inhibitor protein glucokinase regulatory protein (GKRP) and by binding to activator proteins such as 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK2/FBP2); changes in glucokinase expression and activity are associated with poorly controlled type 2 diabetes and nonalcoholic fatty liver disease. [REVIEW] PMID: 27146014
9. PFKFB3 was a novel downstream substrate of mTOR signaling pathway as PFKFB3 level was augmented after knocking down TSC2 in THP1 and OCI-AML3 acute myeloid leukemia cells. PMID: 28082200
10. Taken together, our study identifies PFKFB3 as a key TGFbeta1 effector protein that mediates TGFbeta1's effect on Snail expression, invasion, and glycolysis. PMID: 28161638
11. we investigate the crosstalk between PFKFB3 and TIGAR (TP53-Induced Glycolysis and Apoptosis Regulator), a protein known to protect cells from oxidative stress. Our results show consistent TIGAR induction in HeLa cells in response to PFKFB3 knockdown PMID: 27491040
12. Knockdown of PFKFB3 by siRNAs significantly inhibited the proliferation and migration abilities of gastric cancer cells. Our data suggest that PFKFB3 might be a potential biomarker for gastric cancer and anti-neoplastic targeting gene. PMID: 27983531
13. The targeting of MCT1 and PFKFB3 regulated cell proliferation. PMID: 27373212
14. the novel role of PFKFB3 in induction of paclitaxel resistance by raising lactate production and activating TLR4 signaling, was characterized. PMID: 27262815
15. a novel role of PFKFB3 in glycolytic metabolism and ER stress of OA cartilage explants and chondrocytes PMID: 26718307
16. Our data suggest that chronic inflammation promotes the development of CRC by stimulating aerobic glycolysis and IL-6 is functioning, at least partly, through regulating PFKFB3 at early stage of colorectal cancer (CRC). PMID: 26530697
17. MicroRNA-26b inhibits osteosarcoma cell migration and invasion by down-regulating PFKFB3 expression PMID: 26681033
18. The expression of PFKFB3, PFKFB4, NAMPT, and TSPAN13 is strongly up-regulated in pediatric glioma. PMID: 27491149
19. Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs). PMID: 26337471
20. A binding site for miR-26b was predicted in the 3'UTR of PFKFB3. miR-26b overexpression repressed PFKFB3 mRNA and protein levels. PMID: 25672572
21. Data show that inhibition of AMP-Activated kinase (AMPK) or 6-phosphofructo-2-kinase-fructose-2,6-biphosphatase 3 (PFKFB3) results in enhanced cell death in mitosis. PMID: 26322680
22. Shh regulates PFKFB3 activation in breast cancer cells. PMID: 26171876
23. study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration PMID: 26093295
24. PFKFB3 promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27. PMID: 25032860
25. Shear stress-mediated repression of endothelial cell metabolism via KLF2 and PFKFB3 controls endothelial cell phenotype. PMID: 25359860
26. Because deregulation of endometrial SRC-2 expression has been associated with common gynecological disorders of reproductive-age women, this signaling pathway, involving SRC-2 and PFKFB3 PMID: 24204309
27. Reduced methylation of PFKFB3 shifted glucose utilization from glycolysis toward the pentose phosphate pathway. PMID: 24633012
28. PFKFB3 is a promising target for the reduction of endothelial glycolysis and its related pathological angiogenesis PMID: 24700124
29. blockade of PFKFB3 by the small molecule 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) reduced vessel sprouting. PMID: 24332967
30. PFKFB3 regulates oxidative stress homeostasis via its S-glutathionylation in cancer. PMID: 24295899
31. estrogen receptor directly promotes PFKFB3 mRNA transcription PMID: 24515104
32. Phosphofructokinase deficiency impairs ATP generation, autophagy, and redox balance in rheumatoid arthritis T cells. PMID: 24043759
33. Study showed that endothelial cells (ECs)relied on glycolysis rather than on oxidative phosphorylation for ATP production and that loss of the glycolytic activator PFKFB3 in ECs impaired vessel formation. PMID: 23911327
34. Stress stimuli affect PFKFB3 transcriptional regulation and kinase activation by protein phosphorylation and glycolysis in cancer cells. PMID: 23548149
35. Low bisphosphatase activity of PFKFB3 is solely due to the presence of a serine at residue 302. PMID: 22275052
36. inducible PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. PMID: 21860432
37. The allelic deletion of PFKFB3 resulted in a decrease of PFKFB3 mRNA level accompanied by a lower PFKFB3 protein level. PMID: 21987444
38. The breakdown of PFKFB3 during S phase occurs specifically via a distinct residue (S(273)) within the conserved recognition site for SCF-beta-TrCP. PMID: 22106309
39. Data demonstrate that PFKFB3 is essential for cell division and that it is regulated by APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP. PMID: 21402913
40. Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3. PMID: 20453422
41. Glycolysis-promoting enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, isoform 3 (PFKFB3), is degraded by the E3 ubiquitin ligase APC/C-Cdh1. PMID: 20080744
42. Our results demonstrate that the splice variant UBI2K4 impedes the tumour cell growth and might serve as a tumour suppressor in astrocytic tumours. PMID: 19490427
43. The results show the expression of the pfkfb3 gene in and its downregulation during myogenic cell differentiation, the decrease of uPFK-2 isozyme levels, product of pfkfb3 gene, is due to its degradation through the ubiquitin-proteasome pathway PMID: 12935880
44. PFKFB3 cloning and characterization PMID: 12963966
45. pfkfb3 is a hypoxia-inducible gene that is stimulated through HIF interaction with the consensus HRE site in its promoter region PMID: 15466858
46. kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate PMID: 15896703
47. Findings support a potential role for the phosphorylation of PFKFB3 protein in the progression of cancer and angiogenesis. PMID: 16115917
48. Insulin induces the phosphorylation of PFKFB3 protein, expanding the role of these structurally unique iPFK-2/PFKFB3 isoforms in the metabolic regulation of adipocytes. PMID: 16306349
49. Our results clearly demonstrated the existence of splice isoform of PFKFB-4 mRNA in the DB-1 melanoma cells and its overexpression under hypoxic conditions. PMID: 16311927
50. The results obtained highlight the importance of uPFK-2 on the regulation of glycolysis, on cell viability and proliferation and also on anchorage-independent growth. PMID: 16698023

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HGNC: 8874

OMIM: 605319

KEGG: hsa:5209

STRING: 9606.ENSP00000369100

UniGene: Hs.195471