Product Details

Uniprot No.

Target Names

Alternative Names

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody; 6-bisphosphatase antibody; 6-P2ase 3 antibody; 6-P2ASE brain/placenta-type isozyme antibody; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody; 6PF 2-K/Fru 2,6 P2ase 3 antibody; 6PF-2-K/Fru-2 antibody; F263_HUMAN antibody; fructose 6 phosphate,2 kinase/fructose 2; 6 bisphosphatase antibody; Fructose-2 antibody; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody; iPFK 2 antibody; iPFK-2 antibody; IPFK2 antibody; PFK/FBPase 3 antibody; PFK2 antibody; PFKFB3 antibody; Renal carcinoma antigen NY REN 56 antibody; Renal carcinoma antigen NY-REN-56 antibody; uPFK antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PFKFB3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PFKFB3 serves as a critical regulator of glycolytic flux, functioning as a bifunctional enzyme that controls cellular levels of fructose-2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1. This inducible isoform has emerged as a significant focus in cancer metabolism research, where its overexpression drives the Warburg effect and supports rapid tumor cell proliferation. Beyond oncology, PFKFB3 plays important roles in immune cell activation, angiogenesis, and inflammatory responses, making it a valuable target across multiple research disciplines.

This recombinant monoclonal antibody, generated from clone 15C7 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies.

Validation across multiple platforms demonstrates genuine experimental flexibility. Western blot analysis confirms specific detection of PFKFB3 at the expected 60 kDa molecular weight across diverse human cell lines including A-431, U-251MG, HEK293, HeLa, and U937, with optimal performance at dilutions between 1:500 and 1:2000. Immunohistochemistry studies in paraffin-embedded human kidney tissue reveal clear target localization, while immunofluorescence staining in THP-1 cells enables subcellular visualization of PFKFB3 distribution. Flow cytometry validation using 786-O renal carcinoma cells further extends the utility of this antibody for quantitative single-cell analysis.

Whether investigating metabolic reprogramming in cancer, exploring immune cell function, or studying vascular biology, this antibody provides a dependable tool for characterizing PFKFB3 expression and localization in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Synthesis and degradation of fructose 2,6-bisphosphate.

Gene References into Functions

PI3K-Akt pathway inhibitors, Akti-1/2 and LY294002, reduced PFKFB3 gene induction by PHA, as well as Fru-2,6-P2 and lactate production. Moreover, both inhibitors blocked activation and proliferation in response to PHA, showing the importance of PI3K/Akt signaling pathway in the antigen response of T-lymphocytes. PMID: 29435871
conclude that PFKFB3 bears an oncogene-like regulatory element in lung adenocarcinoma progression PMID: 29327288
Results indicate that PFKFB3 is involved in the proliferation, migration, invasion and angiogenesis of breast cancer. PMID: 29393396
data suggesting that p53 promotes nucleotide biosynthesis in response to DNA damage by repressing the expression of the phosphofructokinase-2 (PFK2) isoform 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), a rate-limiting enzyme that promotes glycolysis. PMID: 27901115
Study provides evidence that PFKFB3 overexpression occupied a dominant position in sorafenib resistance of hepatocellular carcinoma cells. PMID: 28857200
This work has uncovered a novel function of the enzymes PFKFB3 and PFKFB4 in ovarian cancer cells during mitotic arrest PMID: 28152500
PFK-2 seems to be a strategic element that links NADPH oxidase activation and glycolysis modulation, and, as such, is proposed as a potential therapeutic target in inflammatory diseases. PMID: 27799347
Data suggest that hepatic glucokinase activity is regulated by reversible binding to specific inhibitor protein glucokinase regulatory protein (GKRP) and by binding to activator proteins such as 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK2/FBP2); changes in glucokinase expression and activity are associated with poorly controlled type 2 diabetes and nonalcoholic fatty liver disease. [REVIEW] PMID: 27146014
PFKFB3 was a novel downstream substrate of mTOR signaling pathway as PFKFB3 level was augmented after knocking down TSC2 in THP1 and OCI-AML3 acute myeloid leukemia cells. PMID: 28082200
Taken together, our study identifies PFKFB3 as a key TGFbeta1 effector protein that mediates TGFbeta1's effect on Snail expression, invasion, and glycolysis. PMID: 28161638
we investigate the crosstalk between PFKFB3 and TIGAR (TP53-Induced Glycolysis and Apoptosis Regulator), a protein known to protect cells from oxidative stress. Our results show consistent TIGAR induction in HeLa cells in response to PFKFB3 knockdown PMID: 27491040
Knockdown of PFKFB3 by siRNAs significantly inhibited the proliferation and migration abilities of gastric cancer cells. Our data suggest that PFKFB3 might be a potential biomarker for gastric cancer and anti-neoplastic targeting gene. PMID: 27983531
The targeting of MCT1 and PFKFB3 regulated cell proliferation. PMID: 27373212
the novel role of PFKFB3 in induction of paclitaxel resistance by raising lactate production and activating TLR4 signaling, was characterized. PMID: 27262815
a novel role of PFKFB3 in glycolytic metabolism and ER stress of OA cartilage explants and chondrocytes PMID: 26718307
Our data suggest that chronic inflammation promotes the development of CRC by stimulating aerobic glycolysis and IL-6 is functioning, at least partly, through regulating PFKFB3 at early stage of colorectal cancer (CRC). PMID: 26530697
MicroRNA-26b inhibits osteosarcoma cell migration and invasion by down-regulating PFKFB3 expression PMID: 26681033
The expression of PFKFB3, PFKFB4, NAMPT, and TSPAN13 is strongly up-regulated in pediatric glioma. PMID: 27491149
Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs). PMID: 26337471
A binding site for miR-26b was predicted in the 3'UTR of PFKFB3. miR-26b overexpression repressed PFKFB3 mRNA and protein levels. PMID: 25672572
Data show that inhibition of AMP-Activated kinase (AMPK) or 6-phosphofructo-2-kinase-fructose-2,6-biphosphatase 3 (PFKFB3) results in enhanced cell death in mitosis. PMID: 26322680
Shh regulates PFKFB3 activation in breast cancer cells. PMID: 26171876
study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration PMID: 26093295
PFKFB3 promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27. PMID: 25032860
Shear stress-mediated repression of endothelial cell metabolism via KLF2 and PFKFB3 controls endothelial cell phenotype. PMID: 25359860
Because deregulation of endometrial SRC-2 expression has been associated with common gynecological disorders of reproductive-age women, this signaling pathway, involving SRC-2 and PFKFB3 PMID: 24204309
Reduced methylation of PFKFB3 shifted glucose utilization from glycolysis toward the pentose phosphate pathway. PMID: 24633012
PFKFB3 is a promising target for the reduction of endothelial glycolysis and its related pathological angiogenesis PMID: 24700124
blockade of PFKFB3 by the small molecule 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) reduced vessel sprouting. PMID: 24332967
PFKFB3 regulates oxidative stress homeostasis via its S-glutathionylation in cancer. PMID: 24295899
estrogen receptor directly promotes PFKFB3 mRNA transcription PMID: 24515104
Phosphofructokinase deficiency impairs ATP generation, autophagy, and redox balance in rheumatoid arthritis T cells. PMID: 24043759
Study showed that endothelial cells (ECs)relied on glycolysis rather than on oxidative phosphorylation for ATP production and that loss of the glycolytic activator PFKFB3 in ECs impaired vessel formation. PMID: 23911327
Stress stimuli affect PFKFB3 transcriptional regulation and kinase activation by protein phosphorylation and glycolysis in cancer cells. PMID: 23548149
Low bisphosphatase activity of PFKFB3 is solely due to the presence of a serine at residue 302. PMID: 22275052
inducible PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. PMID: 21860432
The allelic deletion of PFKFB3 resulted in a decrease of PFKFB3 mRNA level accompanied by a lower PFKFB3 protein level. PMID: 21987444
The breakdown of PFKFB3 during S phase occurs specifically via a distinct residue (S(273)) within the conserved recognition site for SCF-beta-TrCP. PMID: 22106309
Data demonstrate that PFKFB3 is essential for cell division and that it is regulated by APC/C-Cdh1 and SKP1-CUL1-F (SCF)-beta-TrCP. PMID: 21402913
Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3. PMID: 20453422
Glycolysis-promoting enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, isoform 3 (PFKFB3), is degraded by the E3 ubiquitin ligase APC/C-Cdh1. PMID: 20080744
Our results demonstrate that the splice variant UBI2K4 impedes the tumour cell growth and might serve as a tumour suppressor in astrocytic tumours. PMID: 19490427
The results show the expression of the pfkfb3 gene in and its downregulation during myogenic cell differentiation, the decrease of uPFK-2 isozyme levels, product of pfkfb3 gene, is due to its degradation through the ubiquitin-proteasome pathway PMID: 12935880
PFKFB3 cloning and characterization PMID: 12963966
pfkfb3 is a hypoxia-inducible gene that is stimulated through HIF interaction with the consensus HRE site in its promoter region PMID: 15466858
kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate PMID: 15896703
Findings support a potential role for the phosphorylation of PFKFB3 protein in the progression of cancer and angiogenesis. PMID: 16115917
Insulin induces the phosphorylation of PFKFB3 protein, expanding the role of these structurally unique iPFK-2/PFKFB3 isoforms in the metabolic regulation of adipocytes. PMID: 16306349
Our results clearly demonstrated the existence of splice isoform of PFKFB-4 mRNA in the DB-1 melanoma cells and its overexpression under hypoxic conditions. PMID: 16311927
The results obtained highlight the importance of uPFK-2 on the regulation of glycolysis, on cell viability and proliferation and also on anchorage-independent growth. PMID: 16698023

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Protein Families

Phosphoglycerate mutase family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 8874

OMIM: 605319

KEGG: hsa:5209

STRING: 9606.ENSP00000369100

UniGene: Hs.195471

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Code	CSB-RA552118A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A-431 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HeLa whole cell lysate(30µg), U937 whole cell lysate(30µg) All lanes: PFKFB3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2min IHC image of CSB-RA552118A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of THP-1 cell with CSB-RA552118A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA552118A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PFKFB3 Recombinant Monoclonal Antibody

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Target Background