Recombinant Human Carbonic anhydrase 12 (CA12), partial

Code CSB-BP004367HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP004367HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP004367HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CA12
Uniprot No.
Research Area
Cancer
Alternative Names
CA 12; CA XII; CA-XII; CA12; CAH12_HUMAN; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; Carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM RCC 3.1.3; Tumor antigen HOM-RCC-3.1.3
Species
Homo sapiens (Human)
Expression Region
25-301aa
Target Protein Sequence
APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
Mol. Weight
33.1kD
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Reversible hydration of carbon dioxide.
Gene References into Functions
  1. Our findings indicate that loss of CA XII function should be considered in individuals without CFTR mutations who exhibit CF-like features in the sweat gland and lung. PMID: 26911677
  2. The expression of HIF-1alpha, but not HIF-2alpha, decreased in degenerated disc samples and was positively correlated with carbonic anhydrase 12 expression. PMID: 26901836
  3. mutation CA12(E143K) causes mouth dryness disease due to aberrant CA12 glycosylation. PMID: 26486891
  4. Suggest CAXII as a new secondary marker of the multidrug resistance phenotype that influences Pgp activity directly. PMID: 25686827
  5. an upregulation of CAXII in human T-cell acute lymphoblastic leukemia samples supporting the case that CAXII may represent a new therapeutic target for T-ALL/LL. PMID: 23348702
  6. Serum CAXII levels were significantly higher in lung cancer patients than in healthy controls, providing evidence that CAXII may be a novel sero-diagnostic marker for lung cancer. PMID: 22439015
  7. The expression of CA XII in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients. PMID: 22172588
  8. The development of acidosis with topiramate and zonisamide is not determined by drug dose or by treatment duration, but may be influenced by polymorphisms in the gene for CA type XII. PMID: 21278619
  9. Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12.Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12. PMID: 20132413
  10. Study identified the association of a Glu143Lys mutation in carbonic anhydrase 12 (CA12) with the disease. PMID: 21184099
  11. we demonstrate that the hyperchlohidrosis phenotype is due to a homozygous mutation in CA12, encoding carbonic anhydrase XII. PMID: 21035102
  12. CA12 may be used as a novel prognostic marker in combination with histologic grade of invasive cervical cancer. PMID: 21040567
  13. Carbonic anhydrase XII may affect the capability of invasion and migration of MDA-MB-231 cells, which may be mediated through the p38 MAPK pathway. PMID: 20434230
  14. Suggest that CA XII should be considered a potential prognostic and therapeutic target in medulloblastomas and supratentorial primitive neuroectodermal tumours. PMID: 20398423
  15. Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine. PMID: 20215053
  16. The non-pigmented ciliary epithelial cells from glaucoma eyes expressed higher levels of CAXII, which may be a targeted gene in glaucoma. PMID: 12676895
  17. CA XII showed no or weak immunoreaction in the normal gastric mucosa and was slightly increased in gastric tumors. PMID: 12854129
  18. The interplay between the functional von Hippel-Lindau tumor suppressor and CA IX/CA XII in colorectal tumors seems rather PMID: 15849821
  19. CA12 mRNA expression was detected in 88.1% of cervical cancers. PMID: 16416108
  20. CA XII is commonly expressed in diffuse astrocytomas and that it might be used as a biomarker of poor prognosis. PMID: 18322268
  21. Carbonic anhydrase (CA) IX (and possibly also CA XII) participates in pH regulation, which is important for survival of hypoxic cancer cells. Both enzymes are therefore promising targetable therapeutic molecules. [Review] PMID: 18336315
  22. Crucial role of ER in the regulation of the CA12 gene. PMID: 18451179
  23. In embryonic and early fetal tissues; there is no evidence of co-localization of CAIX and CAXII; CAIX and CAXII expression is closely related to cell origin and secretory activity involving proton transport, respectively. PMID: 19291313

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Involvement in disease
Hyperchlorhidrosis, isolated (HCHLH)
Subcellular Location
Membrane; Single-pass type I membrane protein. Cell membrane.
Protein Families
Alpha-carbonic anhydrase family
Tissue Specificity
Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat gl
Database Links

HGNC: 1371

OMIM: 143860

KEGG: hsa:771

STRING: 9606.ENSP00000178638

UniGene: Hs.210995

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