Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CA12

Uniprot No.

O43570

Research Area

Cancer

Alternative Names

CA 12; CA XII; CA-XII; CA12; CAH12_HUMAN; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; Carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM RCC 3.1.3; Tumor antigen HOM-RCC-3.1.3

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

25-301aa

Target Protein Sequence

APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.1kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this Recombinant Human cry1Ac protein began at the genetic level, where the coding sequence for the cry1Ac protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (Yeast) so that the cells could be cultured and expressed the desired CA12 protein. And we finally got the recombinant CA12 protein with the purity of 90%+ determined by SDS-PAGE.

The carbonic anhydrase 12 (CA12) gene encodes a zinc metalloenzyme that catalyzes reversible carbon dioxide hydration and thereby modulates a range of biological processes. CA12 is a type I membrane protein that has been found to be expressed in renal cell carcinoma and a range of other cancer types. There is some evidence that CA12 can promote tumor growth by driving micro environmental acidification. And some studies have primarily found that CA12 expression is restricted to certain cell types with maximal expression in terminally differentiated cells. In breast cancer patients, intratumoral CA12 upregulation is associated with better patient prognosis, whereas in oral cancer this gene is associated with tumor invasion and metastasis. Currently, CA12 is known to play central roles in regulating many cancers, but its function in the context of different cancers remains to be fully discussed.

Target Background

Function

Reversible hydration of carbon dioxide.

Gene References into Functions

Our findings indicate that loss of CA XII function should be considered in individuals without CFTR mutations who exhibit CF-like features in the sweat gland and lung. PMID: 26911677
The expression of HIF-1alpha, but not HIF-2alpha, decreased in degenerated disc samples and was positively correlated with carbonic anhydrase 12 expression. PMID: 26901836
mutation CA12(E143K) causes mouth dryness disease due to aberrant CA12 glycosylation. PMID: 26486891
Suggest CAXII as a new secondary marker of the multidrug resistance phenotype that influences Pgp activity directly. PMID: 25686827
an upregulation of CAXII in human T-cell acute lymphoblastic leukemia samples supporting the case that CAXII may represent a new therapeutic target for T-ALL/LL. PMID: 23348702
Serum CAXII levels were significantly higher in lung cancer patients than in healthy controls, providing evidence that CAXII may be a novel sero-diagnostic marker for lung cancer. PMID: 22439015
The expression of CA XII in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients. PMID: 22172588
The development of acidosis with topiramate and zonisamide is not determined by drug dose or by treatment duration, but may be influenced by polymorphisms in the gene for CA type XII. PMID: 21278619
Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12.Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12. PMID: 20132413
Study identified the association of a Glu143Lys mutation in carbonic anhydrase 12 (CA12) with the disease. PMID: 21184099
we demonstrate that the hyperchlohidrosis phenotype is due to a homozygous mutation in CA12, encoding carbonic anhydrase XII. PMID: 21035102
CA12 may be used as a novel prognostic marker in combination with histologic grade of invasive cervical cancer. PMID: 21040567
Carbonic anhydrase XII may affect the capability of invasion and migration of MDA-MB-231 cells, which may be mediated through the p38 MAPK pathway. PMID: 20434230
Suggest that CA XII should be considered a potential prognostic and therapeutic target in medulloblastomas and supratentorial primitive neuroectodermal tumours. PMID: 20398423
Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine. PMID: 20215053
The non-pigmented ciliary epithelial cells from glaucoma eyes expressed higher levels of CAXII, which may be a targeted gene in glaucoma. PMID: 12676895
CA XII showed no or weak immunoreaction in the normal gastric mucosa and was slightly increased in gastric tumors. PMID: 12854129
The interplay between the functional von Hippel-Lindau tumor suppressor and CA IX/CA XII in colorectal tumors seems rather PMID: 15849821
CA12 mRNA expression was detected in 88.1% of cervical cancers. PMID: 16416108
CA XII is commonly expressed in diffuse astrocytomas and that it might be used as a biomarker of poor prognosis. PMID: 18322268
Carbonic anhydrase (CA) IX (and possibly also CA XII) participates in pH regulation, which is important for survival of hypoxic cancer cells. Both enzymes are therefore promising targetable therapeutic molecules. [Review] PMID: 18336315
Crucial role of ER in the regulation of the CA12 gene. PMID: 18451179
In embryonic and early fetal tissues; there is no evidence of co-localization of CAIX and CAXII; CAIX and CAXII expression is closely related to cell origin and secretory activity involving proton transport, respectively. PMID: 19291313

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Involvement in disease

Hyperchlorhidrosis, isolated (HCHLH)

Subcellular Location

Membrane; Single-pass type I membrane protein. Cell membrane.

Protein Families

Alpha-carbonic anhydrase family

Tissue Specificity

Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat gl

Database Links

HGNC: 1371

OMIM: 143860

KEGG: hsa:771

STRING: 9606.ENSP00000178638

UniGene: Hs.210995

Code	CSB-YP004367HU
Abbreviation	Recombinant Human CA12 protein, partial
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004367HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CA12. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004367HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CA12.
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Recombinant Human Carbonic anhydrase 12 (CA12), partial

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Target Background

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