Thanks for your inquiry. The four expression systems of NOS3 protein are futures. We have not detected the activity, but can only provide active reference information。
Recombinant Human Nitric oxide synthase, endothelial(NOS3) ,partial
CSB-YP015946HU >> Yeast
CSB-EP015946HU >> E.coli
CSB-BP015946HU >> Baculovirus
CSB-MP015946HU >> Mammalian cell
regional representation:751-1203aa ;C-terminal fragment (including FAD-binding FR-type domain)
Label information:EP&BP&MP:N-terminal 10xHis-tagged and C-terminal Myc-tagged;YP:N-terminal 6xHis-tagged
Expressed sequences:
IHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP
NOS3 active reference information : http://www.jbc.org/content/274/53/37658.long It has been mentioned in the literature that the protein expressed by recombinant pichia pastoris is active, but we have not done this protein nor have we detected it, which cannot guarantee 100% activity.