An ORF cDNA corresponding to the peptide of human protein phosphatase 1 regulatory subunit 15A (PPP1R15A) was expressed with an N-terminal 6xHis-tag in the yeast cells. PPP1R15A CSB-YP018528HU is a truncated molecule having amino acids of Gly40-Gly674. Its purity is greater than 90% measured by SDS-PAGE. A molecular mass band of about 75 kDa was presented on the SDS-PAGE gel under reducing conditions. This recombinant PPP1R15A protein may find uses in the specific antibody synthesis or the studies of epigenetics and nuclear signal transduction.
PPP1R15A, also called GADD34, is transcriptionally induced by stress, downstream of cAMP‐dependent transcription factor ATF4, and C/EBP homologous protein. Its translation escapes the general attenuation of protein synthesis resulting from eIF2α phosphorylation. The stress‐induced eIF2α holohosphatase promotes dephosphorylation of eIF2α in stressed cells, thereby allowing protein synthesis to resume. Thus, PPP1R15A‐PP1 is a crucial effector of the negative feedback loop that shifts cells away from stress to basal activity.
||Greater than 90% as determined by SDS-PAGE.
||Epigenetics and Nuclear Signaling
||Apoptosis associated protein; GADD 34; Growth arrest and DNA damage inducible 34; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog; Ppp1r15a; PR15A_HUMAN; Protein phosphatase 1 regulatory (inhibitor) subunit 15A; Protein phosphatase 1 regulatory subunit 15A
||Homo sapiens (Human)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.