Recombinant Human Ras-related protein Rab-5C (RAB5C)

Code CSB-YP019215HU
MSDS
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Source Yeast
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Code CSB-EP019215HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019215HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP019215HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAB5C
Uniprot No.
Alternative Names
L1880; RAB; member of RAS oncogene family like ; RAB5C; RAB5C; member of RAS oncogene family; RAB5C; member RAS oncogene family; RAB5C_HUMAN; RAB5CL; RAB5L; RABL; Ras-related protein Rab-5C
Species
Homo sapiens (Human)
Expression Region
1-216
Target Protein Sequence
MAGRGGAARP NGPAAGNKIC QFKLVLLGES AVGKSSLVLR FVKGQFHEYQ ESTIGAAFLT QTVCLDDTTV KFEIWDTAGQ ERYHSLAPMY YRGAQAAIVV YDITNTDTFA RAKNWVKELQ RQASPNIVIA LAGNKADLAS KRAVEFQEAQ AYADDNSLLF METSAKTAMN VNEIFMAIAK KLPKNEPQNA TGAPGRNRGV DLQENNPASR SQCCSN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Protein transport. Probably involved in vesicular traffic.
Gene References into Functions
  1. We identify RAB5C as a new candidate gene for type-2 diabetes risk with a functional role in endosomal type-2 diabetes module. Functional analysis shows that RAB5C is tyrosine phosphorylated. Data analysis reveals a tyrosine phosphorylation motif in the GTP binding site of RAB5C which may be targeted by insulin receptor. Insulin-dependent tyrosine phosphorylation of RAB5C is inhibited by overexpression of HACD3 (PTPLAD1). PMID: 30300385
  2. Enforced miR-509 expression in NALM6 cells reduced RAB5C mRNA and protein levels, and RAB5C was demonstrated to be a direct target of miR-509. PMID: 25368993
  3. PLP2 and RAB5C are binding partners of TPD52. PMID: 24604726
  4. Rab5C modulates Rac1-mediated cell motility. PMID: 24587345
  5. GTP-Rab5c also bound to AMAP1, and activation of Rab5c by EGFR signaling was necessary to promote the intracellular association of AMAP1 and PRKD2 PMID: 22734003

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome.
Protein Families
Small GTPase superfamily, Rab family
Database Links

HGNC: 9785

OMIM: 604037

KEGG: hsa:5878

STRING: 9606.ENSP00000345689

UniGene: Hs.650382

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