Product Details

Purity

≥ 95% as determined by SDS-PAGE.≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA.Immobilized Human SIRPB1 at 2 μg/mL can bind anti-SIRPB1 recombinant antibody(CSB-RA021335MA1HU).The EC50 is 0.9675-1.114 ng/mL.
②SIRPB1 Recombinant Monoclonal Antibody(CSB-RA021335MA1HU) captured on Protein A Chip can bind Human SIRPB1 with an affinity constant of 0.245 nM as detected by MetaSPR Assay (WeSPRTM 200).

Target Names

SIRPB1

Uniprot No.

O00241

Alternative Names

Signal-regulatory protein beta-1; SIRP-beta-1; CD172 antigen-like family member B; CD antigen CD172b

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

30-371aa

Target Protein Sequence

EDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPL

Mol. Weight

38.6 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

SIRPB1 functions as an immunomodulatory receptor on myeloid and lymphoid cells, and this mammalian-expressed extracellular domain (aa 30–371) preserves the native glycosylation and disulfide architecture required for physiologically relevant antibody recognition. Functional ELISA demonstrates dose-dependent binding to an anti-SIRPB1 recombinant antibody with an EC50 of 0.97–1.11 ng/mL, while surface plasmon resonance confirms high-affinity interaction at a KD of 0.245 nM, providing quantitative benchmarks for therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody validation in immune checkpoint research. The dual-method purity confirmation (>95% by SDS-PAGE and SEC-HPLC) combined with endotoxin levels below 1.0 EU/μg aligns with standards expected in ligand-binding assays, SPR-based affinity characterization, and biolayer interferometry studies where contaminant interference must be minimized. This partial construct serves as a positive control in receptor-ligand interaction platforms and supports drug discovery workflows targeting SIRP family members in cancer immunotherapy.

Target Background

Function

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.

Gene References into Functions

SIRPB1 copy-number polymorphism was significantly associated with impulsive-disinhibited personality scores in a dose-dependent manner. PMID: 25039969
SIRPbeta1 is a novel IFN-induced microglial receptor that supports clearance of neural debris and Abeta aggregates by stimulating phagocytosis. PMID: 19893026
SIRP beta 1 is a disulfide-linked homodimer in leukocytes that regulates neutrophil transepithelial migration PMID: 16081415
This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Detected in monocytes and dendritic cells.

Database Links

HGNC: 15928

OMIM: 603889

UniGene: Hs.664861

Code	CSB-MP021335HUd7
Abbreviation	Recombinant Human SIRPB1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized Human SIRPB1 at 2 μg/ml can bind anti-SIRPB1 recombinant antibody(CSB-RA021335MA1HU).The EC₅₀ is 0.9675-1.114 ng/mL. Biological Activity Assay Activity SIRPB1 Recombinant Monoclonal Antibody(CSB-RA021335MA1HU) captured on Protein A Chip can bind Human SIRPB1 with an affinity constant of 0.245 nM as detected by MetaSPR Assay (WeSPRTM 200). Biological Activity Assay The purity of SIRPB1 was greater than 95% as determined by SEC-HPLC
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Signal-regulatory protein beta-1 (SIRPB1), partial (Active)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage