Recombinant Human Transcription factor Sp8 (SP8)

Code CSB-YP811623HU
MSDS
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Source Yeast
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Code CSB-EP811623HU
MSDS
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Source E.coli
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Code CSB-EP811623HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811623HU
MSDS
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Source Baculovirus
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Code CSB-MP811623HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
SP8
Uniprot No.
Alternative Names
BTD; Buttonhead; Homolog of Btd; OTTHUMP00000120017; Sp 8; SP8; Sp8 transcription factor; SP8_HUMAN; Specificity protein 8; Trans acting transcription factor 8; Transcription factor Sp8
Species
Homo sapiens (Human)
Expression Region
1-490
Target Protein Sequence
MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SASCNVVGSS LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSSA HSQDGSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAG EVGSAGASSW WDVGAGWIDV QNPNSAAALP GSLHPAAGGL QTSLHSPLGG YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA GAGGSMLSAG PSAPLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGGGGSAGS GSGGKKGSDT DSEHSAAGSP PCHSPELLQP PEPGHRNGLE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
Gene References into Functions
  1. Data suggest expression of transcription factor p8/SP8 is up-regulated in endoplasmic reticulum stress due to lipotoxicity; overexpression of SP8 up-regulates autophagy; autophagy due to lipotoxicity requires SP8; these reactions are mTOR-independent. PMID: 26733200
  2. Genetic variants on 3q21 and in the Sp8 transcription factor gene (SP8) as susceptibility loci for psychotic disorders. PMID: 23967141
  3. This study provided that SP8 are associated with Bipolar I in the Han Chinese population PMID: 20386566
  4. Observational study of gene-disease association. (HuGE Navigator) PMID: 20634891
Subcellular Location
Nucleus.
Protein Families
Sp1 C2H2-type zinc-finger protein family
Database Links

HGNC: 19196

OMIM: 608306

KEGG: hsa:221833

STRING: 9606.ENSP00000408792

UniGene: Hs.195922

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