Recombinant Macaca fascicularis Natural cytotoxicity triggering receptor 3 (NCR3), partial (Active)

NKp30 (NCR3) is a key activating receptor on natural killer cells that recognizes stress-induced ligands on tumor and infected cells, making it a critical target for understanding NK cell-mediated cytotoxicity in primate models. This recombinant protein, spanning the extracellular domain (aa 19–135), demonstrates quantified binding to its cognate ligand NCR3LG1 with an EC50 of 7.4–11.2 ng/mL in functional ELISA, providing a validated basis for ligand-receptor interaction studies and competitive inhibition assays in cynomolgus macaque immunology research. Mammalian expression preserves the native glycosylation and disulfide bonding patterns essential for proper receptor folding, supporting its use in therapeutic antibody epitope mapping, blocking antibody screening, and affinity characterization by surface plasmon resonance or biolayer interferometry. The protein meets quality thresholds commonly required for functional assays, with >95% purity by SDS-PAGE and endotoxin levels <1.0 EU/μg, aligning with standards expected in immune checkpoint research and drug discovery programs targeting NK cell activation pathways.