Code | CSB-EP004937MOW |
Size | US$388 |
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The gene encoding the Rhesus macaque CD40LG protein (44-261aa) is incorporated into a plasmid vector to generate recombinant plasmid, which is then introduced into e.coli cells. The e.coli cells containing the recombinant plasmid are selected and cultured under conditions that stimulate the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. After expression, affinity purification is employed to isolate and purify the recombinant Rhesus macaque CD40LG protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Rhesus macaque CD40LG protein, revealing a purity level exceeding 90%.
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My only questions would be if this is designed to bind receptor in an ELISA. Also for me It cannot be fused to an Fc domain (The His tag is okay)
Any advise?