Recombinant Macaca mulatta CD40 ligand(CD40LG),partial

In Stock
Code CSB-EP004937MOW
Product Type Recombinant Protein
Size US$2466
Uniprot No. P63304
Lead Time 3-7 business days
Relevance Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Storage Buffer Tris-based buffer,50% glycerol
Species Macaca mulatta (Rhesus macaque)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence VYLHRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEEKKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Research Area Immunology
Source E.coli
Gene Names CD40LG
Protein Names Tumor necrosis factor ligand superfamily member 5 CD_antigen: CD154
Expression Region 44-261aa
Tag Info N-terminal 6xHis-tagged
Mol. Weight 28.5 kDa
Protein Description Partial
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.
Subcellular Location Cell membrane, Single-pass type II membrane protein, Cell surface, SUBCELLULAR LOCATION: CD40 ligand, soluble form: Secreted
Protein Families Tumor necrosis factor family
Database Links

KEGG: mcc:574160

STRING: 9544.ENSMMUP00000018111

UniGene: Mmu.3581

Pathway NF-kappa B signaling pathway
T cell receptor signaling pathway

Q&A and Customer Reviews

Q&A
Q:

My only questions would be if this is designed to bind receptor in an ELISA. Also for me It cannot be fused to an Fc domain (The His tag is okay)
Any advise?

A:
Very nice to receive your inquiry. We don't have stock for this protein currently.
What we did with this protein was a complete extracellular domain, 44-261aa, which is a protein that is fused with 6xHis, not a Fc fusion protein.
A ligand containing a soluble form of 113-261aa could theoretically bind to a receptor in ELISA, but we have not verified what you are doing. Generally complete extracellular domain is suitable for many experiments.
Customer Reviews

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