CD40LG

CD40L, also known as CD154, is a type II transmembrane protein with a variable molecular weight between 32-39 kDa. CD40L is mainly expressed on activated CD4+ T cells and platelets, and is a member of TNF superfamily. It is a ligand of CD40. When platelets are activated, they are stored in a granules. CD40L can quickly migrate to the surface of platelets and fall off to become soluble CD40L (sCD40L). Both membrane-bound CD40L (mCD40L) and sCD40L can bind to CD40 to initiate the corresponding downstream immune and inflammatory responses.

After CD40 binds to CD40L, downstream signaling pathways including nuclear transcription factor NF-kB are activated and the expression of pro-inflammatory and pro-thrombotic genes are up-regulated, resulting in a wide range of biological effects, including inhibiting cell apoptosis, inducing cell proliferation, differentiation and activation, and participating in atherosclerotic plaques, tumors and the formation of new blood vessels.

Through this series of biological effects, CD40/CD40L plays an important role in the occurrence and development of cancer and the regulation of immune inflammatory response. Therefore, it has been introduced into the research of a series of related diseases such as tumors, immune diseases and cardiovascular diseases, and provided a new drug target for the treatment of these diseases.

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Validated Data

Purity determined
by SDS-PAGE

Greater than 93% as determined by SDS-PAGE.

The binding activity of antigen-antibody validated by functional ELISA

Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody (CSB-RA156386A0HU), the EC₅₀ is 2.353-3.232 ng/ml.

The binding activity of CD40-CD40L validated by LSPR

Human CD40 protein captured on COOH chip can bind Human CD40L protein with an affinity constant of 2.06 nM as detected by LSPR Assay.

The binding activity of CD40-CD40L validated by functional ELISA

Immobilized CD40 (CSB-MP004936HU1) at 2 μg/ml can bind CD40L, the EC₅₀ is 3.112-3.858 ng/ml.

The following CD40LG reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CD40LG Antibodies

CD40LG Proteins

CD40LG ELISA Kits

CD40LG Antibodies

CD40LG Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA004937GA01HU	CD40LG Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA067336	CD40LG Antibody	Human,Mouse	ELISA,WB
CSB-PA06005A0Rb	CD40LG Antibody	Human	ELISA
CSB-PA06005B0Rb	CD40LG Antibody, HRP conjugated	Human	ELISA

CD40LG Antibodies for Macaca mulatta (Rhesus macaque)

Code	Product Name	Species Reactivity	Application
CSB-PA004937ZA01MOW	CD40LG Antibody	Macaca mulatta	ELISA, WB (ensure identification of antigen)

CD40LG Proteins

CD40LG Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP004937HU1	Recombinant Human CD40 ligand (CD40LG), partial	Yeast
CSB-AP002211HU	Recombinant Human CD40 ligand (CD40LG), partial (Active)	E.coli
CSB-CF004937HU	Recombinant Human CD40 ligand (CD40LG)	in vitro E.coli expression system
CSB-EP004937HU1 CSB-BP004937HU1 CSB-MP004937HU1 CSB-EP004937HU1-B	Recombinant Human CD40 ligand (CD40LG), partial	E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-RP060054h	Recombinant Human CD40 ligand (CD40LG), partial	E.coli
CSB-AP005191HU	Recombinant Human CD40 ligand (CD40LG), partial (Active)	E.coli
CSB-MP004937HU3	Recombinant Human CD40 ligand (CD40LG), partial (Active)	Mammalian cell

CD40LG Proteins for Macaca mulatta (Rhesus macaque)

Code	Product Name	Source
CSB-YP004937MOW CSB-BP004937MOW CSB-MP004937MOW CSB-EP004937MOW-B	Recombinant Macaca mulatta (Rhesus macaque) CD40 ligand (CD40LG), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP004937MOW	Recombinant Macaca mulatta CD40 ligand (CD40LG), partial	E.coli

CD40LG Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP004937PI CSB-EP004937PI CSB-BP004937PI CSB-MP004937PI CSB-EP004937PI-B	Recombinant Pig CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Aotus trivirgatus (Three-striped night monkey) (Douroucouli)

Code	Product Name	Source
CSB-YP004937DNI CSB-EP004937DNI CSB-BP004937DNI CSB-MP004937DNI CSB-EP004937DNI-B	Recombinant Aotus trivirgatus CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Callithrix jacchus (White-tufted-ear marmoset)

Code	Product Name	Source
CSB-YP004937CYL CSB-EP004937CYL CSB-BP004937CYL CSB-MP004937CYL CSB-EP004937CYL-B	Recombinant Callithrix jacchus CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Macaca nemestrina (Pig-tailed macaque)

Code	Product Name	Source
CSB-YP004937MOX CSB-EP004937MOX CSB-BP004937MOX CSB-MP004937MOX CSB-EP004937MOX-B	Recombinant Macaca nemestrina CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP004937CH CSB-EP004937CH CSB-BP004937CH CSB-MP004937CH CSB-EP004937CH-B	Recombinant Chicken CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP004937RA CSB-EP004937RA CSB-BP004937RA CSB-MP004937RA CSB-EP004937RA-B	Recombinant Rat CD40 ligand (Cd40lg), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-CF004937MO	Recombinant Mouse CD40 ligand (Cd40lg)	in vitro E.coli expression system
CSB-YP004937MO1 CSB-EP004937MO1 CSB-BP004937MO1 CSB-MP004937MO1 CSB-EP004937MO1-B	Recombinant Mouse CD40 ligand (Cd40lg), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Felis catus (Cat) (Felis silvestris catus)

Code	Product Name	Source
CSB-CF004937CA	Recombinant Cat CD40 ligand (CD40LG)	in vitro E.coli expression system
CSB-YP004937CA1 CSB-EP004937CA1 CSB-BP004937CA1 CSB-MP004937CA1 CSB-EP004937CA1-B	Recombinant Cat CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Source
CSB-CF004937DO	Recombinant Dog CD40 ligand (CD40LG)	in vitro E.coli expression system
CSB-YP004937DO1 CSB-EP004937DO1 CSB-BP004937DO1 CSB-MP004937DO1 CSB-EP004937DO1-B	Recombinant Dog CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD40LG Proteins for Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys)

Code	Product Name	Source
CSB-CF004937DRI	Recombinant Cercocebus atys CD40 ligand (CD40LG)	in vitro E.coli expression system
CSB-YP004937DRI1 CSB-EP004937DRI1 CSB-BP004937DRI1 CSB-MP004937DRI1 CSB-EP004937DRI1-B	Recombinant Cercocebus atys CD40 ligand (CD40LG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli