Like all recombinant proteins, this Recombinant Mouse Eno1 protein was encoded by recombinant DNA. The recombinant DNA was introduced to a plasmid in which the gene of Eno1 was cloned downstream of a promoter region. When the plasmid was introduced to the cells of E.coli, the E.coli’s own protein synthesis pathways would then result in the expression of the Eno1 protein. And the next step was protein purification. The purity of this recombinant protein is 90%+ determined by SDS-PAGE.
Eno1 is a protein encoding gene that provides an instruction in making a protein named alpha-enolase (also commonly known as enolase 1). The protein encoded by this gene is a key glycolytic enzyme in the cytoplasm of prokaryotic and eukaryotic cells and catalyzes the dehydratation of 2-phosphoglycerate to phosphoenolpyruvate in the last steps of the catabolic glycolytic pathway. It is expressed on the surface of several cell types and acts as a plasminogen receptor, particularly in tumors, contributing to cancer cell proliferation, migration, invasion, and metastasis. This protein is considered a multifunctional protein.
||Greater than 90% as determined by SDS-PAGE.
||Eno1; Eno-1; Alpha-enolase; EC 184.108.40.206; 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
||Mus musculus (Mouse)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.