Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Eno1

Uniprot No.

P17182

Research Area

Signal Transduction

Alternative Names

Eno1; Eno-1; Alpha-enolase; EC 4.2.1.11; 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-433aa

Target Protein Sequence

SILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.4kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Like all recombinant proteins, this Recombinant Mouse Eno1 protein was encoded by recombinant DNA. The recombinant DNA was introduced to a plasmid in which the gene of Eno1 was cloned downstream of a promoter region. When the plasmid was introduced to the cells of E.coli, the E.coli’s own protein synthesis pathways would then result in the expression of the Eno1 protein. And the next step was protein purification. The purity of this recombinant protein is 90%+ determined by SDS-PAGE.

Eno1 is a protein encoding gene that provides an instruction in making a protein named alpha-enolase (also commonly known as enolase 1). The protein encoded by this gene is a key glycolytic enzyme in the cytoplasm of prokaryotic and eukaryotic cells and catalyzes the dehydratation of 2-phosphoglycerate to phosphoenolpyruvate in the last steps of the catabolic glycolytic pathway. It is expressed on the surface of several cell types and acts as a plasminogen receptor, particularly in tumors, contributing to cancer cell proliferation, migration, invasion, and metastasis. This protein is considered a multifunctional protein.

Target Background

Function

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Gene References into Functions

this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection PMID: 28525970
Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions. PMID: 27460355
The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels PMID: 26302382
a Wnt/beta-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair, is reported. PMID: 26035385
Enolase is a new target of liver X receptors. PMID: 25708389
Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic PMID: 25643152
MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
These results demonstrate the novel requirement of alpha-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
ENO1-positive monocytes and macrophages are found in arthritic ankle tissues of mice with collagen-induced arthritis. PMID: 22623332
By concentrating and enhancing plasmin generation on the cell surface, alpha-enolase-type plasminogen receptor is an important component of skeletal myogenesis. PMID: 14515195
the expression of alpha-enolase plasminogen receptor may serve to concentrate and enhance plasmin generation on the cell surface of migratory myoblasts contributing to efficient muscle repair [review] PMID: 15574344
plasminogen stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at alpha-enolase promoter PMID: 16225843

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Subcellular Location

Cytoplasm. Cell membrane.

Protein Families

Enolase family

Tissue Specificity

Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma het

Database Links

KEGG: mmu:13806

STRING: 10090.ENSMUSP00000079727

UniGene: Mm.372357

Code	CSB-EP007670MOb0
Abbreviation	Recombinant Mouse Eno1 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007670MOb0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Eno1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP007670MOb0 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Eno1.
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Recombinant Mouse Alpha-enolase (Eno1), partial

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