Eno1 Antibody

Datasheet

Code	CSB-PA007670HA01MO
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue All lanes: Eno1 antibody at 2.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Eno1 Polyclonal antibody

Uniprot No.

P17182

Target Names

Eno1

Alternative Names

Eno1 antibody; Eno-1 antibody; Alpha-enolase antibody; EC 4.2.1.11 antibody; 2-phospho-D-glycerate hydro-lyase antibody; Enolase 1 antibody; Non-neural enolase antibody; NNE antibody

Raised in

Rabbit

Species Reactivity

Mouse, Human

Immunogen

Recombinant Mouse Alpha-enolase protein (2-433AA)

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

The Eno1 Antibody (Product code: CSB-PA007670HA01MO) is Non-conjugated. For Eno1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007670HB01MO	Eno1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007670HC01MO	Eno1 Antibody, FITC conjugated
Biotin	CSB-PA007670HD01MO	Eno1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

Gene References into Functions

this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection PMID: 28525970
Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions. PMID: 27460355
The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels PMID: 26302382
a Wnt/beta-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair, is reported. PMID: 26035385
Enolase is a new target of liver X receptors. PMID: 25708389
Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic PMID: 25643152
MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
These results demonstrate the novel requirement of alpha-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
ENO1-positive monocytes and macrophages are found in arthritic ankle tissues of mice with collagen-induced arthritis. PMID: 22623332
By concentrating and enhancing plasmin generation on the cell surface, alpha-enolase-type plasminogen receptor is an important component of skeletal myogenesis. PMID: 14515195
the expression of alpha-enolase plasminogen receptor may serve to concentrate and enhance plasmin generation on the cell surface of migratory myoblasts contributing to efficient muscle repair [review] PMID: 15574344
plasminogen stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at alpha-enolase promoter PMID: 16225843

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Subcellular Location

Cytoplasm. Cell membrane.

Protein Families

Enolase family

Tissue Specificity

Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma het

Database Links

KEGG: mmu:13806

STRING: 10090.ENSMUSP00000079727

UniGene: Mm.372357

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Related pathways

HIF-1 signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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