Eno1 Antibody, HRP conjugated

Code CSB-PA007670HB01MO
Size US$166
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  • Western Blot
    Positive WB detected in: PC-3 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, Rat heart tissue, Rat brain tissue, Rat skeletal muscle tissue, Mouse kidney tissue, Mouse liver tissue
    All lanes: Eno1 antibody; HRP conjugated at 0.42µg/ml
    Predicted band size: 48 kDa
    Observed band size: 48, 34 kDa
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Eno1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
Eno1 antibody; Eno-1 antibody; Alpha-enolase antibody; EC 4.2.1.11 antibody; 2-phospho-D-glycerate hydro-lyase antibody; Enolase 1 antibody; Non-neural enolase antibody; NNE antibody
Raised in
Rabbit
Species Reactivity
Mouse, Rat, Human
Immunogen
Recombinant Mouse Alpha-enolase protein (2-433AA)
Immunogen Species
Mus musculus (Mouse)
Conjugate
HRP
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Gene References into Functions
  1. this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection PMID: 28525970
  2. Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions. PMID: 27460355
  3. The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process PMID: 27966485
  4. Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels PMID: 26302382
  5. a Wnt/beta-catenin-driven functional role of ENO1 and PDIA3 in alveolar epithelial cell plasticity in lung injury and repair, is reported. PMID: 26035385
  6. Enolase is a new target of liver X receptors. PMID: 25708389
  7. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic PMID: 25643152
  8. MBP1 inhibits Ab fibril formation in vitro and demonstrate the ability of MBP1 to reduce Ab pathology and improve behavioral performance. PMID: 25457550
  9. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
  10. These results demonstrate the novel requirement of alpha-enolase for restoring homeostasis of injured muscle tissue, by controlling the pericellular localization of plasmin activity. PMID: 23239981
  11. ENO1-positive monocytes and macrophages are found in arthritic ankle tissues of mice with collagen-induced arthritis. PMID: 22623332
  12. By concentrating and enhancing plasmin generation on the cell surface, alpha-enolase-type plasminogen receptor is an important component of skeletal myogenesis. PMID: 14515195
  13. the expression of alpha-enolase plasminogen receptor may serve to concentrate and enhance plasmin generation on the cell surface of migratory myoblasts contributing to efficient muscle repair [review] PMID: 15574344
  14. plasminogen stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at alpha-enolase promoter PMID: 16225843

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Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
Enolase family
Tissue Specificity
Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma het
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