Recombinant Mouse Calpain-1 catalytic subunit (Capn1)

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Code CSB-EP004490MO
MSDS
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Capn1
Uniprot No.
Research Area
Others
Alternative Names
Capn1; Canp1; Capa1Calpain-1 catalytic subunit; EC 3.4.22.52; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; muCANP
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-713aa
Target Protein Sequence
MTEELITPVYCTGVSAQVQKKRDKELGLGRHENAIKYLGQDYETLRARCLQSGVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWGEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEKVLSEEEIDDNFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKLLDTDLDGVVTFDLFKWLQLTMFA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
86.1kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-388' and 'His-410'.
Gene References into Functions
  1. Calpain-1 deficiency impairs mGluR-dependent LTD and fear memory extinction. PMID: 28202907
  2. calpain contributes to the regulation of new gene expression that is necessary for these memory processes as a regulator of Ca(2+)-signal transduction pathway. PMID: 29258546
  3. the present study suggested that ephrinB/EphB signaling contributes to spinal nociceptive processing via the actions of calpain1 and caspase3. PMID: 29749521
  4. Authors provide the first evidence that calpain-1 regulates platelet hyperactivity in sickle mice. PMID: 29101791
  5. A total of 55 genes were differentially expressed in micro-calpain knockout mice. PMID: 29775750
  6. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
  7. indicate that calpain-1 and calpain-2 play opposite roles in high increased intraocular pressure-induced ischemic injury PMID: 27185592
  8. Results strengthen the roles of both calpain-1 and PHLPP1 in synaptic plasticity and learning and memory, and illustrate the complexities of the interactions between multiple pathways participating in synaptic plasticity. PMID: 27421891
  9. Genetic ablation of calpain-1 ameliorates chronic pain behavior in sickle cell mice. PMID: 27418647
  10. Our results indicate that calpain mediates cytokine-induced collagen-I synthesis and proliferation of ASMCs via the mTORC2/Akt signalling pathway, thereby regulating airway smooth muscle remodelling in asthma. PMID: 27649066
  11. mutations in CAPN1 are an additional cause of ataxia in mammals, including humans. PMID: 27320912
  12. Results indicate that calpain-1 and calpain-2 are differentially activated in hippocampus following seizure activity, with calpain-1 activation being limited to a small population of interneurons, and possibly triggering neuroprotective events, while calpain-2 is widely activated in pyramidal neurons of CA1 and CA3, and triggers neurodegenerative cascades. PMID: 27622212
  13. Here we show the combined loss of glial glutamate transporters GLT1 and GLAST in spinal cord caused motor neuronal death and hindlimb paralysis. PMID: 28821644
  14. These results suggested that Streptococcus pneuomoniae PLY induces the influx of calcium in Streptococcus pneumoniae-infected macrophages, followed by calpain activation and subsequent IL-1alpha maturation and secretion. PMID: 28630064
  15. High calpain expression is associated with brain injury. PMID: 27875293
  16. the expression of the full-length 60-kDa calcineurin protein is down-regulated due to over-activated calpain that cleaves calcineurin to form a 45-kDa fragment. PMID: 27171192
  17. calpains and calpastatin in patients with idiopathic pulmonary arterial hypertension (PAH) and mice with hypoxic or spontaneous (SM22-5HTT(+) strain) PH, were investigated. PMID: 26974350
  18. Attenuation of atherosclerotic lesions/inflammation in ApoE KO mice by simvastatin might be associated with the downregulation of CD36 and calpain-1. PMID: 28448973
  19. Leukocyte calpain-1/calpain-2 deficiency reduces angiotensin II-induced inflammation and atherosclerosis but not abdominal Aortic aneurysms in mice. PMID: 26966280
  20. Taken together, our findings suggested that DHRS7C maintains intracellular Ca(2+) homeostasis involving the ER/SR and that functional loss of DHRS7C leads to Ca(2+) overload in the cytosol and ER/SR, resulting in enlarged cellular morphology via calpain activation PMID: 27806939
  21. In conclusion, calpain was involved in oxygen glucose deprivation-induced RGC-5 necroptosis with the increased expression of its downstream molecule tAIF. PMID: 27752886
  22. CRYbetaA3/A1-crystallin has a role in preventing nuclear cataract impaired lysosomal cargo clearance and calpain activation PMID: 26863613
  23. Capn1 protein and activity in mitochondria were elevated in diabetic mouse hearts. Increased mitochondrial Capn1 was associated with increased mitochondrial ROS generation and oxidative damage and reduced ATP5A1 protein and ATP synthase activity. PMID: 26470784
  24. LOX-1 is, at least in part, responsible for the inhibitory effect of ox-LDL on macrophage migration and this process involves calpain-1 and -2. PMID: 26393906
  25. Over-expression of Cast inhibits calpain activation and attenuates post-infarction myocardial remodeling. PMID: 25786109
  26. Junctophilin-2 is cleaved by calpain at multiple sites, resulting in dysfunctional junctophilin-2 truncations. PMID: 26063807
  27. Data indicate that calpain-1 up-regulation induces endoplasmic reticulum (ER) stress. PMID: 25660447
  28. Calcitriol stimulates expression of mu-calpain in co-cultured mouse cells. PMID: 24687633
  29. Data suggest that vitamin D3 up-regulates skeletal muscle fat deposition, up-regulates muscle u-calpain/Capn1 and m-calpain/Capn2 expression, and down-regulates muscle calpastatin expression. PMID: 25520983
  30. Calpain-mediated MFN2 degradation is a novel mechanism regulating mitochondrial fusion during glutamate excitotoxicity. PMID: 25416777
  31. in spinal cord of 30 day old mice, activation of calpain-1 produces high amounts of nNOS active species, while in 120 day old mice enhanced-prolonged activation of calpain-1 inactivates nNOS and down-regulates NR2B PMID: 25151305
  32. Overexpression of the calpain-specific inhibitor calpastatin reduces human alpha-synuclein processing, aggregation and synaptic impairment in transgenic mice. PMID: 24619358
  33. Calpain-2 compensation promotes angiotensin II-induced ascending and abdominal aortic aneurysms in calpain-1 deficient mice. PMID: 23977256
  34. Our findings support the therapeutic promise of highly specific calpain inhibition in the treatment of tauopathies and other neurodegenerative states. PMID: 25009256
  35. The suppressive effects of PDGF-BB on Ca(2+) overload in neurons were more potent than those of PDGF-AA. PMID: 24454980
  36. calpain-1 is identified as a novel regulator in IgE-mediated mast cell activation and could serve as a potential therapeutic target for the management of allergic inflammation. PMID: 24760147
  37. The results of the present study highlight a dual role for calpain-1 in the weaned gland after the pregnancy/lactation cycle, controlling programmed cell death and participating in the epigenetic programme during adipocyte differentiation. PMID: 24467364
  38. Calpain induced myocardial NF-kappaB activation, TNF-alpha expression, and myocardial dysfunction in septic mice through IkappaBalpha protein cleavage. PMID: 24441549
  39. These data contribute to the existing evidence of the importance of the calpain system's involvement in muscle growth, development, and atrophy. PMID: 23798514
  40. synaptic NMDAR-coupled mu-calpain activation is neuroprotective PMID: 24285894
  41. The calpain/calpastatin system has opposing roles in growth and metastatic dissemination of melanoma. PMID: 23565252
  42. data suggest that skNAC controls myoblast migration and sarcomere architecture in a calpain-dependent manner PMID: 23662692
  43. Activated skeletal muscle calpain regulates acetylcholine release in motor nerve terminals via nitric oxide and neural microRNAs, a novel mechanism for the modulation of synaptic activity in normal or pathological conditions. PMID: 23616539
  44. Both CAPN-1 and -2 were induced after weaning and its activity increased in isolated mitochondria and lysosomes. PMID: 22555453
  45. This study demonistrated that conditional model of calpain deficiency in the CNS has provided a clearer understanding of calpain biology in the brain regarding CNS development, synaptic plasticity, and specific neuronal death paradigms. PMID: 23536090
  46. Calpain-1 gene deletion improved RBC deformability without any effect on RBC lifespan. Ankyrin, band 3, protein 4.1R, adducin & dematin were not degraded. K+-Cl- co-transporter & the Gardos channel were reduced. PMID: 22870887
  47. Potentiation of the platelet spreading phenotype in calpain-1(-/-) mice suggests a novel role of calpain-1 in hemostasis. PMID: 22458296
  48. Increased STEP and calpain activation contribute to altered NMDAR localization in an Huntington's disease mouse model. PMID: 22523092
  49. Increased lipid peroxidation and low-density lipoprotein oxidation in the brain of hyperlipidemic mice are associated with increased activation of calpain-1/2. PMID: 21836084
  50. Calpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A-induced neuronal growth cone collapse. PMID: 22547824

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Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
Peptidase C2 family
Database Links
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