Recombinant Mouse Caspase-3 (Casp3)

Code CSB-YP004548MO
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Source Yeast
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Code CSB-EP004548MO
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Source E.coli
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Code CSB-EP004548MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004548MO
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Source Baculovirus
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Code CSB-MP004548MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Casp3
Uniprot No.
Alternative Names
Casp3; Cpp32Caspase-3; CASP-3; EC 3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
Species
Mus musculus (Mouse)
Expression Region
29-175
Target Protein Sequence
SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Gene References into Functions
  1. Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID: 30451870
  2. Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418
  3. Because DFNA5-induced secondary necrosis and GSDMD-induced pyroptosis are dependent on CASP3 activation, we propose that they are forms of programmed necrosis. PMID: 28045099
  4. Results revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells and suggest that casp3 is activated by oocyte-G1 promoting male germ cell apoptosis. PMID: 29802994
  5. NKG2D is upregulated, and ROS and caspase-3 are inhibited by Strongylocentrotus nudus egg polysaccharide-enhanced antitumor 5-FU in mice PMID: 27385218
  6. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
  7. Caspases and their substrates are key mediators of apoptosis. PMID: 27552481
  8. SERT protects trophoblast cells against apoptotic cell death in caspase-3 independent pathway by terminating the 5-HT signaling. PMID: 28109119
  9. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
  10. Induction of apoptosis in epithelial cancer cells was accompanied by a switch from glycolysis to oxidative phosphorylation, cytosol acidification and caspase-3 activation. PMID: 28063999
  11. This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525
  12. ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396
  13. These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival. PMID: 28150874
  14. report that mutating the caspase-3 consensus site in the EAAT2 sequence with an aspartate to asparagine mutation PMID: 28342750
  15. melatonin could inhibit TLR4 expression in hypoxic microglia followed by suppression of caspase3 activation leading to decrease in production of proinflammatory mediators PMID: 27812200
  16. A significant emergence of GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice 2 weeks after the MSC injection. PMID: 27802273
  17. Conversely, treatment with LY294002 (a selective inhibitor of Akt1) reversed the effects of quercetin. In conclusion, these findings highlight the important role of quercetin in protecting against cognitive deficits and inhibiting neuronal apoptosis via the Akt signaling pathway. We believe that quercetin might prove to be a useful therapeutic component in treating cerebral I/R diseases in the near future. PMID: 27450812
  18. cognitive decline was significantly correlated with increased levels of caspase activity and tau truncated by caspase-3 PMID: 27220334
  19. this study shows that Le Carbone, a charcoal supplement, attenuates experimental colitis through caspase 3 dependent apoptotic pathways PMID: 27978459
  20. Cepharanthine treatment reduced mitochondrial membrane potential and upregulated the level of cleaved caspases, including caspase-9 and caspase-3/7, in a dose-dependent fashion. PMID: 27121492
  21. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. PMID: 26861667
  22. caspase-3 is essential for a subset of social behaviors, but despite similar hyper-locomotion in both sexes, only male Casp3(-/-) mice exhibited social interaction deficits, which is interesting given the male bias of autism PMID: 26783106
  23. Study identified CASP-3 mRNA as a new miRNA-155 target in lipopolysaccharides-activated macrophages suggesting that through destabilization of CASP-3, miR-155 contributes to the protective role to sustain macrophage function in inflammation response. PMID: 26574931
  24. does not significantly contribute to cardiomyocyte death induced by transient coronary occlusion PMID: 26924441
  25. expression level of miR-98, which can regulate Caspase-3, was significantly decreased. Huwe1, the host gene of miR-98, was positively associated with miR-98 expression after Silica NP exposure PMID: 26263183
  26. administration of 0.3 IU/mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF/VEGFR2 PMID: 26539488
  27. that caspase-3-mediated nuclear opening formation accompanied by histone release from the opening is a critical step toward chromatin condensation during erythropoiesis in mice PMID: 26954545
  28. MicroRNA-124 and microRNA-137 cooperatively control caspase-3 activity through BCL2L13 in hippocampal neural stem cells. PMID: 26207921
  29. impaired mTORC1-Homer-3 activity underlies PC susceptibility in Spinocerebellar ataxia type 1 and presents a promising therapeutic target PMID: 26748090
  30. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
  31. The study demonstrates sublethal caspase-activation has an important role in cardiomyocyte differentiation and may have significant implications for promoting cardiac regeneration after myocardial injury involving exogenous or endogenous cell sources. PMID: 25763592
  32. Parkinson disease induction increased the expression of the apoptotic mediators p53 and active caspase-3 in gastrocnemius muscle. PMID: 25704481
  33. The results reveal that executioner caspases can modulate heart's cellularity and maturation during development, contributing novel information about caspase biology and heart development. PMID: 26121671
  34. Caspase-3 activity was significantly elevated after I/R in both postischemic groups PMID: 26009812
  35. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. PMID: 26504199
  36. a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-kappaB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3 PMID: 26526615
  37. results indicate a novel role of Casp-2 in the osteoclast as a modulator of total and mitochondrial ROS and osteoclast differentiation PMID: 25796569
  38. The results of this study showed that the Casp3 gene is important for the proper function of the semicircular canals but less important for the otolith organ function. PMID: 25827332
  39. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PMID: 25748204
  40. Caspase 3 cleavage of Pax7 has a role in inhibiting self-renewal of satellite cells PMID: 26372956
  41. deletion of p21Cip1/Waf1 and caspase-3 accelerates proliferation and partially rescues the differentiation defects of caspase-3 deficient hematopoietic stem cells PMID: 25286245
  42. The hepatic expression level of cleaved caspase-3 protein in the group treated concomitantly with doxorubicin and theanine was significantly lower than that in the doxorubicin treated group. PMID: 25680506
  43. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver PMID: 25575574
  44. Interleukin-1beta enhances FasL-induced caspase-3/-7 activity without increasing apoptosis in primary mouse hepatocytes PMID: 25551609
  45. Induction of cIAP2 expression upon microglia activation prevents the conversion of caspase-3 p19 subunit to p17 subunit. PMID: 25501826
  46. Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice. PMID: 25964561
  47. miR-21 is endowed with anti-apoptotic properties by suppressing the expression of PDCD4 gene and active caspase 3/8 fragments in the condition of renal IRI PMID: 25322693
  48. Results suggest that miR-378 overexpression attenuates high glucose-suppressed osteogenic differentiation through targeting CASP3 and activating the PI3K/Akt pathway. PMID: 25400823
  49. Results show that human or mouse cells exposed to ionizing radiation or other stresses can survive with persistent caspase-3 activation, which in turn promotes genetic instability and oncogenic transformation. PMID: 25866249
  50. cancer stimulates p-Stat3 in muscle, activating protein loss by stimulating caspase-3, myostatin, and the ubiquitin-proteasome system. PMID: 25787076

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase C14A family
Tissue Specificity
Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
Database Links
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