Mouse Caspase 3,Casp-3 ELISA Kit

Code CSB-E08858m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P70677
Lead Time 3-5 working days
Abbreviation CASP3
Protein Biological Process 1 Apoptosis/Autophagy
Target Name caspase 3, apoptosis-related cysteine peptidase
Alias CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
Species Mus musculus (Mouse)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %93
Range %87-97
1:4Average %103
Range %98-107
1:8Average %90
Range %84-95
Recovery
The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-105
EDTA plasma (n=4)9288-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.637 2.552 2.595 2.437
102.175 2.106 2.141 1.983
51.464 1.524 1.494 1.336
2.50.832 0.829 0.831 0.673
1.250.543 0.524 0.534 0.376
0.6250.366 0.339 0.353 0.195
0.3120.269 0.285 0.277 0.119
00.159 0.157 0.158  
References
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
Database Links

KEGG: mmu:12367

STRING: 10090.ENSMUSP00000091238

UniGene: Mm.34405

Pathway MAPK signaling pathway
p53 signaling pathway
TNF signaling pathway
Apoptosis
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
AGE-RAGE signaling pathway in diabetic complications
ER Stress pathway

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