Mouse Caspase 3,Casp-3 ELISA Kit

Instructions
Code CSB-E08858m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name caspase 3, apoptosis-related cysteine peptidase
Alternative Names Casp3 ELISA Kit; Cpp32Caspase-3 ELISA Kit; CASP-3 ELISA Kit; EC 3.4.22.56 ELISA Kit; Apopain ELISA Kit; Cysteine protease CPP32 ELISA Kit; CPP-32 ELISA Kit; LICE ELISA Kit; Protein Yama ELISA Kit; SREBP cleavage activity 1 ELISA Kit; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12] ELISA Kit
Abbreviation CASP3
Uniprot No. P70677
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse caspase 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %91-99
1:2Average %93
Range %87-97
1:4Average %103
Range %98-107
1:8Average %90
Range %84-95
Recovery
The recovery of mouse caspase 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-105
EDTA plasma (n=4)9288-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.637 2.552 2.595 2.437
102.175 2.106 2.141 1.983
51.464 1.524 1.494 1.336
2.50.832 0.829 0.831 0.673
1.250.543 0.524 0.534 0.376
0.6250.366 0.339 0.353 0.195
0.3120.269 0.285 0.277 0.119
00.159 0.157 0.158  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Gene References into Functions
  1. Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID: 30451870
  2. Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418
  3. Because DFNA5-induced secondary necrosis and GSDMD-induced pyroptosis are dependent on CASP3 activation, we propose that they are forms of programmed necrosis. PMID: 28045099
  4. Results revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells and suggest that casp3 is activated by oocyte-G1 promoting male germ cell apoptosis. PMID: 29802994
  5. NKG2D is upregulated, and ROS and caspase-3 are inhibited by Strongylocentrotus nudus egg polysaccharide-enhanced antitumor 5-FU in mice PMID: 27385218
  6. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
  7. Caspases and their substrates are key mediators of apoptosis. PMID: 27552481
  8. SERT protects trophoblast cells against apoptotic cell death in caspase-3 independent pathway by terminating the 5-HT signaling. PMID: 28109119
  9. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
  10. Induction of apoptosis in epithelial cancer cells was accompanied by a switch from glycolysis to oxidative phosphorylation, cytosol acidification and caspase-3 activation. PMID: 28063999
  11. This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525
  12. ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396
  13. These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival. PMID: 28150874
  14. report that mutating the caspase-3 consensus site in the EAAT2 sequence with an aspartate to asparagine mutation PMID: 28342750
  15. melatonin could inhibit TLR4 expression in hypoxic microglia followed by suppression of caspase3 activation leading to decrease in production of proinflammatory mediators PMID: 27812200
  16. A significant emergence of GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice 2 weeks after the MSC injection. PMID: 27802273
  17. Conversely, treatment with LY294002 (a selective inhibitor of Akt1) reversed the effects of quercetin. In conclusion, these findings highlight the important role of quercetin in protecting against cognitive deficits and inhibiting neuronal apoptosis via the Akt signaling pathway. We believe that quercetin might prove to be a useful therapeutic component in treating cerebral I/R diseases in the near future. PMID: 27450812
  18. cognitive decline was significantly correlated with increased levels of caspase activity and tau truncated by caspase-3 PMID: 27220334
  19. this study shows that Le Carbone, a charcoal supplement, attenuates experimental colitis through caspase 3 dependent apoptotic pathways PMID: 27978459
  20. Cepharanthine treatment reduced mitochondrial membrane potential and upregulated the level of cleaved caspases, including caspase-9 and caspase-3/7, in a dose-dependent fashion. PMID: 27121492
  21. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. PMID: 26861667
  22. caspase-3 is essential for a subset of social behaviors, but despite similar hyper-locomotion in both sexes, only male Casp3(-/-) mice exhibited social interaction deficits, which is interesting given the male bias of autism PMID: 26783106
  23. Study identified CASP-3 mRNA as a new miRNA-155 target in lipopolysaccharides-activated macrophages suggesting that through destabilization of CASP-3, miR-155 contributes to the protective role to sustain macrophage function in inflammation response. PMID: 26574931
  24. does not significantly contribute to cardiomyocyte death induced by transient coronary occlusion PMID: 26924441
  25. expression level of miR-98, which can regulate Caspase-3, was significantly decreased. Huwe1, the host gene of miR-98, was positively associated with miR-98 expression after Silica NP exposure PMID: 26263183
  26. administration of 0.3 IU/mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF/VEGFR2 PMID: 26539488
  27. that caspase-3-mediated nuclear opening formation accompanied by histone release from the opening is a critical step toward chromatin condensation during erythropoiesis in mice PMID: 26954545
  28. MicroRNA-124 and microRNA-137 cooperatively control caspase-3 activity through BCL2L13 in hippocampal neural stem cells. PMID: 26207921
  29. impaired mTORC1-Homer-3 activity underlies PC susceptibility in Spinocerebellar ataxia type 1 and presents a promising therapeutic target PMID: 26748090
  30. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
  31. The study demonstrates sublethal caspase-activation has an important role in cardiomyocyte differentiation and may have significant implications for promoting cardiac regeneration after myocardial injury involving exogenous or endogenous cell sources. PMID: 25763592
  32. Parkinson disease induction increased the expression of the apoptotic mediators p53 and active caspase-3 in gastrocnemius muscle. PMID: 25704481
  33. The results reveal that executioner caspases can modulate heart's cellularity and maturation during development, contributing novel information about caspase biology and heart development. PMID: 26121671
  34. Caspase-3 activity was significantly elevated after I/R in both postischemic groups PMID: 26009812
  35. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. PMID: 26504199
  36. a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-kappaB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3 PMID: 26526615
  37. results indicate a novel role of Casp-2 in the osteoclast as a modulator of total and mitochondrial ROS and osteoclast differentiation PMID: 25796569
  38. The results of this study showed that the Casp3 gene is important for the proper function of the semicircular canals but less important for the otolith organ function. PMID: 25827332
  39. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PMID: 25748204
  40. Caspase 3 cleavage of Pax7 has a role in inhibiting self-renewal of satellite cells PMID: 26372956
  41. deletion of p21Cip1/Waf1 and caspase-3 accelerates proliferation and partially rescues the differentiation defects of caspase-3 deficient hematopoietic stem cells PMID: 25286245
  42. The hepatic expression level of cleaved caspase-3 protein in the group treated concomitantly with doxorubicin and theanine was significantly lower than that in the doxorubicin treated group. PMID: 25680506
  43. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver PMID: 25575574
  44. Interleukin-1beta enhances FasL-induced caspase-3/-7 activity without increasing apoptosis in primary mouse hepatocytes PMID: 25551609
  45. Induction of cIAP2 expression upon microglia activation prevents the conversion of caspase-3 p19 subunit to p17 subunit. PMID: 25501826
  46. Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice. PMID: 25964561
  47. miR-21 is endowed with anti-apoptotic properties by suppressing the expression of PDCD4 gene and active caspase 3/8 fragments in the condition of renal IRI PMID: 25322693
  48. Results suggest that miR-378 overexpression attenuates high glucose-suppressed osteogenic differentiation through targeting CASP3 and activating the PI3K/Akt pathway. PMID: 25400823
  49. Results show that human or mouse cells exposed to ionizing radiation or other stresses can survive with persistent caspase-3 activation, which in turn promotes genetic instability and oncogenic transformation. PMID: 25866249
  50. cancer stimulates p-Stat3 in muscle, activating protein loss by stimulating caspase-3, myostatin, and the ubiquitin-proteasome system. PMID: 25787076

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
Database Links

KEGG: mmu:12367

STRING: 10090.ENSMUSP00000091238

UniGene: Mm.34405

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