Recombinant Mouse Interleukin-12 subunit alpha(Il12a)

Code CSB-YP011586MO
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Source Yeast
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Code CSB-EP011586MO
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Source E.coli
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Code CSB-EP011586MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011586MO
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Source Baculovirus
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Code CSB-MP011586MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Il12a
Uniprot No. P43431
Alternative Names Il12a; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35
Species Mus musculus (Mouse)
Expression Region 23-215
Target Protein Sequence RVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. IL-12 receptor signalling in keratinocytes initiates a protective transcriptional programme that limits skin inflammation. PMID: 27892456
  2. The authors found that the expressions of the mRNAs of both IL-12p35 and IL-12p40 were significantly reduced in the oxygen-induced retinopathy retinas compared to that of the room air-raised control. The results suggest that IL-12 plays important roles in inhibiting pathological retinal neovascularization. PMID: 27312090
  3. IL-35-transduced adipocyte-derived mesenchymal stem cells in vivo can enhance proliferation of CD4(+) CD25(+) Treg cells and suppress the function of effector T cells such as T helper (Th) 1, Th2 and Th17 cells and may reduce the development of allograft rejection. PMID: 28888053
  4. findings show that IL-12 and other proinflammatory cytokines transduce signals through the TCR signalosome in a manner that requires Fyn activity and self-peptide-MHC (self-pMHC) interactions. PMID: 27521342
  5. this study shows that a systemic IL-12 primes intestinal cells that became more prone to develop inflammatory responses, which is long-lasting because intestinal cells maintain their inflammatory potential and their ability to aggravate colitis PMID: 27891587
  6. These results suggest a correlation between CRMP2 phosphorylation and AD pathophysiology, and indicate the effectiveness of pioglitazone treatment at the pre-Abeta accumulation stage in AD model mice. PMID: 27059136
  7. Data show that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) expressing plasmid downregulates the expression of pro-inflammatory cytokines and upregulates the expression of anti-inflammatory cytokines. PMID: 27460435
  8. Data suggest that interleukin-35 (Epstein-Barr virus-induced gene 3 (EBI3) and the interleukin-12 Subunit p35 (p35) subunit) contributes to the increased susceptibility to secondary pneumococcal pneumonia. PMID: 26844658
  9. Deficiency of IL-12p35 limited AMI-induced cardiac injury by promoting pro-angiogenesis and anti-inflammatory functions of monocytes PMID: 26614777
  10. IL-12 immunomodulation delays the onset of lethal peritoneal disease of ovarian cancer. PMID: 26430781
  11. There is a common program of effector T cell differentiation that is regulated by IL-2 and IL-12 signaling and the combined activities of the transcriptional regulators Blimp-1 and T-bet. PMID: 26950239
  12. Irradiation-induced IL-6 can decrease IL-12 production through the inhibition of C-Rel phosphorylation by the IL-6/STAT3 signaling pathway. PMID: 26745884
  13. The IL-12 response by dendritic cells against S. aureus is highly growth phase dependent. PMID: 26872029
  14. These results provide a new pathway of Th1 response regulation where IL-12 secreted by dendritic cellss is consumed by a sub-population of IL-12Rbeta2-expressing Treg cells. PMID: 26745371
  15. These findings reveal previously unappreciated roles for IL-35 in limiting anti-tumor immunity and contributing to T cell dysfunction in the tumor microenvironment. PMID: 26872697
  16. IL-12p35 has a protective role against pulmonary infection with methicillin-resistant Staphylococcus aureus. PMID: 26248370
  17. TBI-induced IL12 augments Tc17 cell-mediated tumor immunity and underline the substantial implications of in vitro preparation of antitumor Tc17 cells with IL12 in the design of T-cell immunotherapies PMID: 25904754
  18. Interleukin-12-producing CD103+ CD11b- CD8+ dendritic cells are responsible for eliciting gut intraepithelial lymphocyte response against Encephalitozoon cuniculi. PMID: 26416905
  19. Interferon-gamma (IFN-gamma) and interleukin-12 (IL-12) levels in the tumors and plasma were significantly enhanced after processing with recombinant mouse CCL19. PMID: 25661385
  20. IL-35 subunits (p35 and EBI3) have the immunoregulatory role in systemic lupus erythematosus. PMID: 25845911
  21. Taken together, IL32gamma will be useful as an adjuvant to boost the cytotoxicities of NK and NKT cells that play critical roles in antitumor immunity. PMID: 25858316
  22. These findings reveal a critical role for IL-12 in shaping the anti-inflammatory biofilm milieu by promoting MDSC recruitment. PMID: 25762781
  23. Results suggest that p35 helps in maintaining the testicular immune privilege, in part in an IL-35-dependent manner. PMID: 24760014
  24. IL-35 mitigates murine acute graft-versus-host disease with retention of graft-versus-leukemia effects. PMID: 25363669
  25. IL-12 production by Batf3-dependent CD103+ dendritic cells is crucial for maintenance of local Th1 immunity against L. major infection. PMID: 25312824
  26. Data (including data from knockout mice) suggest Ebi3 (Epstein-Barr virus induced protein 3) and Il12a (interleukin-12 subunit p35) down-regulate helper-inducer T-lymphocyte (Th17 cell) function in immune response to Listeria monocytogenes. PMID: 24068935
  27. Interleukin-35 induces regulatory B cells that suppress autoimmune disease. [il-35] PMID: 24743305
  28. Dap12- and FcRgamma-deficiency exacerbates Granulocyte-Macrophage Colony-Stimulating Factor-driven monocyte differentiation and production of inflammatory monocyte-derived dendritic cells. PMID: 24155889
  29. Data indicate that while diminishing IL-12 and IL-23 secretion, sphingosine-1-phosphate (S1P) enhanced IL-27 production in dendritic cells (DCs). PMID: 24434636
  30. Whereas both IL-12/Th1 and IL-23/Th17 are involved in cholangitis, it is the IL-12/Th1 signaling pathway that elicits pathology. PMID: 24040208
  31. knockout of p35 in a transgenic Alzheimer mouse model leads to higher mortality rates and exhibited impaired spatial learning and memory at 6 months of age. Also a a reduction in the expression of synaptic markers such as NMDAR1, synaptophysin and GluR1. PMID: 23905994
  32. IL-12a induces the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
  33. IL-12 and type I interferon prolong the division of activated CD8 T cells by maintaining high-affinity IL-2 signaling in vivo. PMID: 24367005
  34. C5a regulates IL-12(+)dendritic cell migration to induce pathogenic Th1 and Th17 cells in sepsis. PMID: 23936099
  35. Cdk5 and p35 are expressed in a murine odontoblast-enriched primary preparation of cells from teeth PMID: 23668392
  36. Anti-CD200R2, anti-IL-9, anti-IL-35, or anti-TGF-beta abolishes increased graft survival and Treg induction induced in cromolyn-treated CD200R1KO.CD200tg mice, and suggest combined role of mast cells and Tregs. PMID: 24142033
  37. Liver sinusoidal endothelial cells undergo immunological maturation exclusively in response to TLR1/2 ligand stimulation. This is achieved by regulating the balance between PD-L1 & IL-12 expression. PMID: 24227786
  38. These data indicate that IL-12 has a nonprotective role in the acute phase and that IL-12 deficiency results in the accelerated formation of a protective capsule during the healing phase, which is considered crucial for early recovery from disease. PMID: 23532102
  39. IL-12 and IFN-alpha differentially program CD8 T cells to re-express distinct levels of PD-1 upon re-encountering Ag, resulting in IL-12-stimulated cells being less susceptible to exhaustion in the face of sustained tumor Ag. PMID: 23804712
  40. IL-12 and IL-2 enhance the IFN-gamma expression reduce the colony load and increase the survival rate in invasive pulmonary aspergillosis mouse model. PMID: 22834482
  41. Data indicate that NK1R-dendritic cells (DCs) promote cellular immunity that requires IL-12p70. PMID: 23365459
  42. The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine production. PMID: 23297419
  43. The expansion of CD8 T cells depends on type I IFN, type I IFN and IL-12 or is largely independent of the two cytokines. PMID: 22815848
  44. WISP-1 exerts paracrine action on immune cells by inhibiting their response to IL12 PMID: 22777646
  45. neither IL-12p40 nor IL-12p35 molecules are required for prion disease-associated neurodegeneration or neuroinflammation PMID: 22787236
  46. Inductive microRNA-21 impairs anti-mycobacterial responses by targeting IL-12 and Bcl-2 PMID: 22710123
  47. Deficiency in interleukin-12p35 regulates angiotensin II-induced cardiac fibrosis by promoting CD4(+) T-cell-dependent differentiation of M2 macrophages and production of transforming growth factor-beta. PMID: 22556333
  48. Systemic IL-12 administration alters hepatic dendritic cell stimulation capabilities. PMID: 22428016
  49. IL-35 production by inducible costimulator-positive Treg cells can suppress IL-17 production and thereby reverse established, IL-17-dependent airway hyperresponsiveness in mice. PMID: 21906794
  50. Gene silencing of IL-12 in dendritic cells was shown to selectively inhibit T cell proliferation on recall responses. PMID: 22289162

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: mmu:16159

STRING: 10090.ENSMUSP00000029345

UniGene: Mm.103783

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