Recombinant Mouse Matrix metalloproteinase-9(Mmp9)

Code CSB-YP014679MO
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Source Yeast
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Code CSB-EP014679MO
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Source E.coli
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Code CSB-EP014679MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014679MO
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Source Baculovirus
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Code CSB-MP014679MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Mmp9
Uniprot No. P41245
Alternative Names Mmp9; Clg4bMatrix metalloproteinase-9; MMP-9; EC 3.4.24.35; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Species Mus musculus (Mouse)
Expression Region 108-730
Target Protein Sequence FQT FKGLKWDHHN ITYWIQNYSE DLPRDMIDDA FARAFAVWGE VAPLTFTRVY GPEADIVIQF GVAEHGDGYP FDGKDGLLAH AFPPGAGVQG DAHFDDDELW SLGKGVVIPT YYGNSNGAPC HFPFTFEGRS YSACTTDGRN DGTPWCSTTA DYDKDGKFGF CPSERLYTEH GNGEGKPCVF PFIFEGRSYS ACTTKGRSDG YRWCATTANY DQDKLYGFCP TRVDATVVGG NSAGELCVFP FVFLGKQYSS CTSDGRRDGR LWCATTSNFD TDKKWGFCPD QGYSLFLVAA HEFGHALGLD HSSVPEALMY PLYSYLEGFP LNKDDIDGIQ YLYGRGSKPD PRPPATTTTE PQPTAPPTMC PTIPPTAYPT VGPTVGPTGA PSPGPTSSPS PGPTGAPSPG PTAPPTAGSS EASTESLSPA DNPCNVDVFD AIAEIQGALH FFKDGWYWKF LNHRGSPLQG PFLTARTWPA LPATLDSAFE DPQTKRVFFF SGRQMWVYTG KTVLGPRSLD KLGLGPEVTH VSGLLPRRLG KALLFSKGRV WRFDLKSQKV DPQSVIRVDK EFSGVPWNSH DIFQYQDKAY FCHGKFFWRV SFQNEVNKVD HEVNQVDDVG YVTYDLLQCP
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).
Gene References into Functions
  1. omega-3 reduces MMP-9 gene expression and improves myoblast engraftment, satellite cell activation, and muscle regeneration by mechanisms involving, at least in part, the regulation of macrophages. PMID: 28623422
  2. These results indicated that MMP-9/2 activation in spinal microglia plays a key role in incision-induced mechanical allodynia in mice. PMID: 29081070
  3. PARP-1, via manipulating the binding of NF-kB/AP-1 at the MMP-9 promoter, regulates MMP-9 expression, which helps maintain mitochondrial homeostasis. PMID: 28478229
  4. fracture union in matrix metalloproteinase 9 deficient mice PMID: 29851987
  5. Study in inflammatory bowel disease (IBD) Mmp-9 knock-out mice model found no differences in clinical or histopathological parameters after genetic or pharmacological inhibition of MMP-9. Therefore suggesting that MMP-9 upregulation is a consequence of the inflammatory process and unlikely represents a therapeutic target in IBD. PMID: 28561062
  6. our findings demonstrate that MMP9 is important for tooth development and DSP is a novel target of MMP9 during dentinogenesis. PMID: 28195206
  7. The findings support the correlation between age-related hearing loss and cognitive decline in C57BL/6J mice, and indicated that MMP9 expression in the auditory cortex and hippocampus may be associated with the underlying mechanisms. PMID: 29901198
  8. Our data suggest that MMP-9 deficiency does not result in major abnormalities in the development of any conventionally selected or agonist selected subsets and does not interfere with thymocyte apoptosis and clearance, and that MMP-9 expression is not induced in immature T cells at any stage of their thymic development. PMID: 27432536
  9. analysis of Foxn1 and Mmp-9 expression in the intact and postinjured skin of young, adult, and old C57BL/6J and transgenic Foxn1::Egfp mice PMID: 28371152
  10. Myocardial MMP-9 inhibition prevents ventricular arrhythmia through pleiotropic effects, including the modulation of calcium homeostasis and reduced calcium leakage. PMID: 27966586
  11. results first identified the role of SNX10 in MMP9 trafficking and secretion, and provided an evidence for SNX10 as a possible therapeutic target for bone destructing disease. PMID: 28498635
  12. Thus, the light reintroduction-induced increase in MMP-9 removes constraints on structural and functional plasticity in the mature cortex. PMID: 28875930
  13. the ZnT3 null state removed synaptic zinc, it rather increased free zinc in the cytosol of brain cells, which appeared to increase MMP-9 activity and BDNF levels. The present results suggest that zinc dyshomeostasis during the critical period of brain development may be a possible contributing mechanism for ASD. PMID: 27352957
  14. via binding to hypoxia-responsive elements in MMP9 gene, HIF1alpha stimulated MMP9 expression, and therefore appeared as a prominent intermediary in HB-EGF-induced blood-brain barrier damage PMID: 27431094
  15. Studied effects of hesperidin on skin photoaging in a hairless mouse model; results showed that hesperidin inhibited the MMP-9 related signaling pathway activated by UVB irradiation. PMID: 29382339
  16. MMP-9 deficiency augmented AngII-induced Abdominal Aortic Aneurysms. PMID: 28420827
  17. Study identifies the tumor suppressor role of epithelial derived-MMP9 in colitis associated cancer via novel mechanistic pathway "MMP9-Notch1-ARF-p53 axis" regulating apoptosis, cell-cycle arrest and DNA damage. PMID: 27861153
  18. These results show that MMP-9/TIMP-1 system disturbance and changes of histological structure in uteri tissue are involved in fluoride-induced reproductive dysfunctions. PMID: 28064417
  19. Synaptic levels of MMP-9 are increased following overexpression of miR-132 in hippocampal neurons. PMID: 26319558
  20. M. tuberculosis infection caused enhanced MMP-1, -9, and miR-223 expression, with inhibited BMAL1 expression. MiR-223 modulated BMAL1 expression via the direct binding of BMAL1 3'-UTR. PMID: 28543681
  21. Results show that MMP-9 modulates cholesterol metabolism, at least in part, through a novel MMP-9-plasma secreted phospholipase A2 axis that affects the hepatic transcriptional responses to dietary cholesterol. Furthermore, the data suggest that dysregulation of the MMP system can result in metabolic disorder, which could lead to atherosclerosis and coronary heart disease. PMID: 27694328
  22. These data document a novel role for MMP-9-dependent proteolysis: the regulation of several aspects of circuit maturation to constrain excitability throughout life. PMID: 26093382
  23. NPY deficient mice had significantly impaired Hematopoietic stem/progenitor cell (HSPC) mobilization due to increased expression of HSPC maintenance factors by reduction of matrix metalloproteinase-9 (MMP-9) activity in bone marrow. PMID: 27090492
  24. Optical imaging demonstrated increased MMP activity in TB lesions and MMP-9 was significantly expressed in cavitary lesions. PMID: 27482816
  25. Grooved surfaces showed time-dependent increase in soluble mediators involved in cell fusion, CCL2 and MMP-9 PMID: 27102570
  26. A MMP-9-cleaved OPN fragment, OPN-32kDa, was responsible for inducing expansion of myeloid-derived suppressor cells, which may contribute to 3LL tumor's evasion of the immune response. PMID: 28986261
  27. p63alpha protein up-regulates heat shock protein 70 expression via E2F1 transcription factor 1, promoting Wasf3/Wave3/MMP9 signaling and bladder cancer invasion PMID: 28794159
  28. IL-33-induced MMP-9 expression in the mouse monocyte/macrophage line RAW264.7. PMID: 28105703
  29. key role in the process of heterotopic ossification PMID: 26919547
  30. MMP-9's contribution to development of atherosclerotic lesions may be a direct stimulation of endothelial cells, and that PAR-1 may serve as a receptor for MMP-9. PMID: 28166283
  31. Results show that ablation of systemic MMP-9 initiated fatal communication between maintenance of physiological functions of MMP-9 in the bone marrow and invasive growth of pancreatic ductal adenocarcinoma via the deregulation of IL6. PMID: 27489361
  32. The MURC/Cavin-4 in vascular smooth muscle cells modulates abdominal aortic aneurysm (AAA) progression at the early stage via the activation of JNK and MMP-9. MURC/Cavin-4 is a potential therapeutic target against AAA progression. PMID: 28433630
  33. In diabetes, transcription of retinal MMP-9 is maintained, in part, by an active DNA methylation-hydroxymethylation process, and regulation of this machinery should help maintain mitochondrial homeostasis and inhibit the development/progression of diabetic retinopathy. PMID: 27454437
  34. early MMP-9 inhibition delayed inflammation resolution and exacerbated cardiac dysfunction, highlighting the importance of using translational approaches in mice. PMID: 27746126
  35. the beta2-adrenoceptor contributes to collagen remodeling during muscle regeneration by decreasing MMP-9 activity. PMID: 26896238
  36. Seminal vesicles were evaluated by morphological and immunohistochemical parameters; androgenic receptor (AR), Insulin-like growth factor 1 (IGFR-1) and metalloproteinase 9 (MMP-9). Intense AR reactivity was seen in both stroma and epithelial regions in the TRAMP 22 group. Intense IGFR-1 and MMP-9 stromal immunolabeling was identified in both TRAMP groups PMID: 27036326
  37. MMP-9 inhibition results from a joint contribution between the components of the extract from Chilean Propolis and Pinocembrin PMID: 27119082
  38. The results suggested that the relieving effect of KGLY against LPS-induced ALI might be partially due to suppression of oxidative stress and inflammatory response, inhibition of TLR4-mediated NF-kappaB activation, and down-regulation of MMP9 expression, indicating it may be a potential therapeutic agent for ALI. PMID: 27036629
  39. peritubular myoid cells exhibit part of the cross-talk which takes place between tumor and seminiferous peritubular myoid cells to regulate matrix-metalloproteinase 9 expression, emphasizing the important role of TNF-a as a crucial signaling component. PMID: 26711538
  40. Matrix metalloproteinase-9 deletion rescues auditory evoked potential habituation deficit in a mouse model of Fragile X Syndrome. PMID: 26850918
  41. Rosuvastatin inhibits MMP-9 expression by upregulating miR-497 in HUVECs and apoE knockout mice, and the combination of rosuvastatin and probucol enhances this effect. PMID: 26502925
  42. data reveal a new cell-signaling role for MMP-9 through CD36 degradation to regulate macrophage phagocytosis and neutrophil apoptosis. PMID: 26578544
  43. Curcumin improves bone microarchitecture in glucocorticoid-induced secondary osteoporosis mice through the activation of microRNA-365 and suppression MMP-9 activity. PMID: 26884838
  44. RhoA-PLD1 signaling is involved in acidic extracellular pH-induced matrix metalloproteinase-9 in mouse metastatic B16-BL6 melanoma cells PMID: 26782071
  45. Our results suggest that MMP-9 deletion may reduce age-related myocardial stiffness, which may explain improved cardiac function in MMP-9 null animals. PMID: 26415707
  46. In diabetic retinopathy transcription of MMP-9 is regulated by AP-1 binding at both, proximal and distal sites of its promoter, and acetylation of c-Jun and c-Fos subunits is important in its regulation. PMID: 26599598
  47. Found hyperexpression of exogenous hypoxia response element-matrix metalloproteinase-9 under the control of hypoxia, and its expression was mainly located in neurons and astrocytes without aggravation of blood brain barrier damage. PMID: 25975730
  48. CXCR4 inhibitor attenuates allergen-induced lung inflammation by down-regulating MMP-9 and ERK1/2 expression. PMID: 26261552
  49. MMP-9 mediates thrombus-induced loss of vein wall compliance by increasing stiffness of the extracellular matrix and collagen-elastin fibers during thrombus resolution. PMID: 26406902
  50. the principal H3NT protease of osteoclastogenesis is matrix metalloproteinase 9 (MMP-9 PMID: 26744418
  51. the present study shows that sildenafil modulates inflammation, with the involvement of MMP-9, MCP-1, and CCR-2, and also contributes to myelin repair. PMID: 26515692
  52. Matrix Metalloproteinase-9 Protects Islets from Amyloid-induced Toxicity. PMID: 26483547
  53. exosomes that are released during exercise contain microRNAs (mir455, mir29b, mir323-5p and mir466) that bind to the 3' region of MMP9 and downregulate its expression. PMID: 25824442
  54. Data suggest a role for MMP-9 in modulation of colonic epithelial permeability and inflammation via MLCK. PMID: 26514773
  55. Selective allosteric inhibition of MMP9 is efficacious in preclinical models of ulcerative colitis and colorectal cancer. PMID: 25961845
  56. Only MMP-9 Rs3918242 (C->T) single nucleotide polymorphism was found to play a significant role in the development of AMD, and the effect was more pronounced at the age of less than 65 years. PMID: 24079541
  57. Results define the cardiac ageing inflammatory signature and assign MMP-9 roles in mediating the inflammaging profile by indirectly and directly modifying macrophage polarization. PMID: 25883218
  58. intestinal microbes with the capacity to produce collagenases and to activate host metalloproteinase MMP9 may break down collagen in the intestinal tissue contributing to anastomotic leak PMID: 25947163
  59. This study showed that Annexin A11 knockdown inhibits the in vitro proliferation and cell apoptosis of Hca-F cell via Akt2/FoxO1 and/or MMP-9 expression pathway. PMID: 25776480
  60. tissue MMP9 has a crucial role in endothelial progenitor cells -induced vascular remodeling after stroke PMID: 26219597
  61. MMP9 knockout mice developed less white matter injury in a subarachnoid hemorrhage model. In wild type mice, MMP9 expression was increased after subarachnoid hemorrhage . PMID: 26374478
  62. Studied whether H2S supplementation reduces NMDA-R and MMP-9-induced dysfunction in diabetic kidney. PMID: 25659756
  63. This study aimed to investigate the effects of the extracellular matrix-degrading enzyme gelatinase B/matrix metalloproteinase-9 (Mmp-9) on islet function in mice. PMID: 25665793
  64. Cytokine-induced MMP-9 activity specifically at the inflammatory border collectively act to accelerate leukocyte chemotaxis across the parenchymal border. PMID: 25704809
  65. TLR2 plays a detrimental role in intracerebral hemorrhage-induced brain damage by activating MMP9 in astrocytes. PMID: 25879213
  66. Data suggest that MMP-9 released by injured neurons favors glia activation; glial cells in turn reinforce their reactive state via autocrine MMP-9 release, contributing to nigro-striatal pathway degeneration. PMID: 24558048
  67. RAB37 regulates the exocytosis of TIMP1 in a nucleotide-dependent manner to inactivate MMP9 migration axis in vitro and in vivo and to suppress tumor metastasis. PMID: 25183545
  68. vascular expression of MMP-9 was significantly higher in RNF213-/- mice than in wild-type mice after common carotid artery ligation PMID: 25383461
  69. MMP-9 gene ablation mitigates hyperhomocystenemia-induced cognition and hearing dysfunction. PMID: 24874304
  70. MMP-9 deletion has a cardioprotective role against lipopolysaccharide exposure, by attenuating macrophage mediated inflammation. PMID: 25240641
  71. Increased neutrophil infiltration and MPO activity was noted in the CM group compared with AB 2 days post injury. PMID: 26225631
  72. Wnt3a and MMP9 induce C3H10T1/2 cells differentiation into cardiomyocyte-like cells. PMID: 25854560
  73. Data provide new evidence supporting a critical role of MMP-9 in liver regeneration after partial hepatectomy through activation of EGFR signaling. PMID: 25956184
  74. the downregulation of MMP-9 by apigenin was mediated by the AKT/p70S6K1 pathway. PMID: 22837693
  75. Loss of BMC-derived or non-BMC-derived MMP9 impairs necrotic and fibroadipose tissue clearance after femoral artery ligation, despite normal arteriogenic and angiogenic vascular growth. PMID: 24582703
  76. Tissue-infiltrating neutrophils constitute the major in vivo source of angiogenesis-inducing MMP-9 in the tumor microenvironment. PMID: 25379015
  77. CXCR6 is essential for pressure overload-mediated myocardial recruitment of monocytes, which contributes to cardiac fibrosis through TNF-alpha-dependent MMP9 activation and collagen synthesis. PMID: 25400729
  78. endothelin-1 is critical for maintaining normal contractile function, for controlling superoxide and Mmp9 levels. PMID: 25848038
  79. The CS activity decreased in the infarcted tissue of wild-type (WT) mice at day 1 post-MI (p<0.05), but this was not observed in the MMP-9 null mice, suggesting that MMP-9 deletion helps to maintain the mitochondrial activity post-MI PMID: 24382150
  80. metalloprotease-9 activation plays a critical role in these high-fat diet effects PMID: 24935427
  81. Knockdown of caveolin-1 inhibited tissue plasminogen activator-induced MMP-9 mRNA up-regulation. PMID: 25683686
  82. MMP-9 knockout in diabetic mice significantly attenuated nephropathy changes. Overexpression of endogenous MMP-9 induced podocyte dedifferentiation. PMID: 24670409
  83. MMP-9 activity disrupts vascular integrity at least partially through a PECAM-1 dependent mechanism and interferes with regeneration of steatotic livers after ischemia/reperfusion injury. PMID: 24412604
  84. reduction of insulin-like growth factor 1 and upregulation of matrix metalloproteinase-9 may contribute to brain ABCA1 deficiency-induced BBB and WM/axonal damage in the ischemic brain PMID: 25593138
  85. Authors show that increased synthesis of MMP9 at the leading edge of migrating epithelium is regulated by galectin-3. PMID: 24829150
  86. MMP-9 expression is more critical than MMP-2 in mediating TGF-beta-induced anterior subcapsular cataract formation. PMID: 24814605
  87. CONCLUSIONS: These results show that targeting and down regulation of MMP-9 by AM9D could prove useful as a therapy against breast carcinoma tumor growth and invasion. PMID: 23407024
  88. NOX2 is involved in the pathogenesis of human emphysema and macrophage-specific NOX2 participates in elastase-induced emphysema through the involvement of sirtuin 1/metalloproteinase-9 pathways in mice PMID: 25116588
  89. Selective inhibition of matrix metalloproteinase-9 attenuates secondary damage resulting from severe traumatic brain injury. PMID: 24194849
  90. These data suggest that in vivo MMP9-mediated processing of HSPB1 acts to regulate VEGF-induced endothelial cells activation for tumor progression, releasing anti-angiogenic HSPB1 fragments. PMID: 24465581
  91. MMP-9 deficiency improved left ventricular function in post-myocardial infarction. PMID: 24768766
  92. Lp(a)/apo(a) inhibits plasminogen activation and regulates matrix metalloproteinase-9 activation and macrophage recruitment. PMID: 24650562
  93. 1alpha,25-(OH)2D3 administered at a physiological relevant concentration promoted osteoclast formation and could regulate osteoclast bone metabolism by increasing matrix metalloproteinase-9 protein expression during osteoclast differentiation. PMID: 24136216
  94. a pathophysiological link between oxLDL and MMP-9 expression in microglia-related neuroinflammation PMID: 24780563
  95. Studied acute mechanical overload effect on expression of IGF-I and MMP-9 in 3D tissue-engineered skeletal muscle. PMID: 24563297
  96. Matrix metalloproteinase (MMP)-9 in cancer-associated fibroblasts (CAFs) is suppressed by omega-3 polyunsaturated fatty acids in vitro and in vivo. PMID: 24586907
  97. Ablation of MMP9 gene ameliorates paracellular permeability and fibrinogen-amyloid beta complex formation during hyperhomocysteinemia. PMID: 24865997
  98. TNF-alpha-induced MMP-9 expression is mediated through a c-Src-dependent PDGFR transactivation. PMID: 24361597
  99. MMP-9 is critical to the mechanisms responsible for neural and non-neural aspects of the fragile X syndrome phenotype. PMID: 25057190
  100. TNF-alpha-induced MMP-9 expression may contribute to the production of sICAM-1 by MC3T3-E1 cells. PMID: 24502696

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Peptidase M10A family
Database Links

KEGG: mmu:17395

STRING: 10090.ENSMUSP00000017881

UniGene: Mm.4406

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