Mouse matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Instructions
Code CSB-E08007m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Alternative Names Mmp9 ELISA Kit; Clg4bMatrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
Abbreviation MMP9
Uniprot No. P41245
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.552 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).
Gene References into Functions
  1. omega-3 reduces MMP-9 gene expression and improves myoblast engraftment, satellite cell activation, and muscle regeneration by mechanisms involving, at least in part, the regulation of macrophages. PMID: 28623422
  2. These results indicated that MMP-9/2 activation in spinal microglia plays a key role in incision-induced mechanical allodynia in mice. PMID: 29081070
  3. PARP-1, via manipulating the binding of NF-kB/AP-1 at the MMP-9 promoter, regulates MMP-9 expression, which helps maintain mitochondrial homeostasis. PMID: 28478229
  4. fracture union in matrix metalloproteinase 9 deficient mice PMID: 29851987
  5. Study in inflammatory bowel disease (IBD) Mmp-9 knock-out mice model found no differences in clinical or histopathological parameters after genetic or pharmacological inhibition of MMP-9. Therefore suggesting that MMP-9 upregulation is a consequence of the inflammatory process and unlikely represents a therapeutic target in IBD. PMID: 28561062
  6. our findings demonstrate that MMP9 is important for tooth development and DSP is a novel target of MMP9 during dentinogenesis. PMID: 28195206
  7. The findings support the correlation between age-related hearing loss and cognitive decline in C57BL/6J mice, and indicated that MMP9 expression in the auditory cortex and hippocampus may be associated with the underlying mechanisms. PMID: 29901198
  8. Our data suggest that MMP-9 deficiency does not result in major abnormalities in the development of any conventionally selected or agonist selected subsets and does not interfere with thymocyte apoptosis and clearance, and that MMP-9 expression is not induced in immature T cells at any stage of their thymic development. PMID: 27432536
  9. analysis of Foxn1 and Mmp-9 expression in the intact and postinjured skin of young, adult, and old C57BL/6J and transgenic Foxn1::Egfp mice PMID: 28371152
  10. Myocardial MMP-9 inhibition prevents ventricular arrhythmia through pleiotropic effects, including the modulation of calcium homeostasis and reduced calcium leakage. PMID: 27966586
  11. results first identified the role of SNX10 in MMP9 trafficking and secretion, and provided an evidence for SNX10 as a possible therapeutic target for bone destructing disease. PMID: 28498635
  12. Thus, the light reintroduction-induced increase in MMP-9 removes constraints on structural and functional plasticity in the mature cortex. PMID: 28875930
  13. the ZnT3 null state removed synaptic zinc, it rather increased free zinc in the cytosol of brain cells, which appeared to increase MMP-9 activity and BDNF levels. The present results suggest that zinc dyshomeostasis during the critical period of brain development may be a possible contributing mechanism for ASD. PMID: 27352957
  14. via binding to hypoxia-responsive elements in MMP9 gene, HIF1alpha stimulated MMP9 expression, and therefore appeared as a prominent intermediary in HB-EGF-induced blood-brain barrier damage PMID: 27431094
  15. Studied effects of hesperidin on skin photoaging in a hairless mouse model; results showed that hesperidin inhibited the MMP-9 related signaling pathway activated by UVB irradiation. PMID: 29382339
  16. MMP-9 deficiency augmented AngII-induced Abdominal Aortic Aneurysms. PMID: 28420827
  17. Study identifies the tumor suppressor role of epithelial derived-MMP9 in colitis associated cancer via novel mechanistic pathway "MMP9-Notch1-ARF-p53 axis" regulating apoptosis, cell-cycle arrest and DNA damage. PMID: 27861153
  18. These results show that MMP-9/TIMP-1 system disturbance and changes of histological structure in uteri tissue are involved in fluoride-induced reproductive dysfunctions. PMID: 28064417
  19. Synaptic levels of MMP-9 are increased following overexpression of miR-132 in hippocampal neurons. PMID: 26319558
  20. M. tuberculosis infection caused enhanced MMP-1, -9, and miR-223 expression, with inhibited BMAL1 expression. MiR-223 modulated BMAL1 expression via the direct binding of BMAL1 3'-UTR. PMID: 28543681
  21. Results show that MMP-9 modulates cholesterol metabolism, at least in part, through a novel MMP-9-plasma secreted phospholipase A2 axis that affects the hepatic transcriptional responses to dietary cholesterol. Furthermore, the data suggest that dysregulation of the MMP system can result in metabolic disorder, which could lead to atherosclerosis and coronary heart disease. PMID: 27694328
  22. These data document a novel role for MMP-9-dependent proteolysis: the regulation of several aspects of circuit maturation to constrain excitability throughout life. PMID: 26093382
  23. NPY deficient mice had significantly impaired Hematopoietic stem/progenitor cell (HSPC) mobilization due to increased expression of HSPC maintenance factors by reduction of matrix metalloproteinase-9 (MMP-9) activity in bone marrow. PMID: 27090492
  24. Optical imaging demonstrated increased MMP activity in TB lesions and MMP-9 was significantly expressed in cavitary lesions. PMID: 27482816
  25. Grooved surfaces showed time-dependent increase in soluble mediators involved in cell fusion, CCL2 and MMP-9 PMID: 27102570
  26. A MMP-9-cleaved OPN fragment, OPN-32kDa, was responsible for inducing expansion of myeloid-derived suppressor cells, which may contribute to 3LL tumor's evasion of the immune response. PMID: 28986261
  27. p63alpha protein up-regulates heat shock protein 70 expression via E2F1 transcription factor 1, promoting Wasf3/Wave3/MMP9 signaling and bladder cancer invasion PMID: 28794159
  28. IL-33-induced MMP-9 expression in the mouse monocyte/macrophage line RAW264.7. PMID: 28105703
  29. key role in the process of heterotopic ossification PMID: 26919547
  30. MMP-9's contribution to development of atherosclerotic lesions may be a direct stimulation of endothelial cells, and that PAR-1 may serve as a receptor for MMP-9. PMID: 28166283
  31. Results show that ablation of systemic MMP-9 initiated fatal communication between maintenance of physiological functions of MMP-9 in the bone marrow and invasive growth of pancreatic ductal adenocarcinoma via the deregulation of IL6. PMID: 27489361
  32. The MURC/Cavin-4 in vascular smooth muscle cells modulates abdominal aortic aneurysm (AAA) progression at the early stage via the activation of JNK and MMP-9. MURC/Cavin-4 is a potential therapeutic target against AAA progression. PMID: 28433630
  33. In diabetes, transcription of retinal MMP-9 is maintained, in part, by an active DNA methylation-hydroxymethylation process, and regulation of this machinery should help maintain mitochondrial homeostasis and inhibit the development/progression of diabetic retinopathy. PMID: 27454437
  34. early MMP-9 inhibition delayed inflammation resolution and exacerbated cardiac dysfunction, highlighting the importance of using translational approaches in mice. PMID: 27746126
  35. the beta2-adrenoceptor contributes to collagen remodeling during muscle regeneration by decreasing MMP-9 activity. PMID: 26896238
  36. Seminal vesicles were evaluated by morphological and immunohistochemical parameters; androgenic receptor (AR), Insulin-like growth factor 1 (IGFR-1) and metalloproteinase 9 (MMP-9). Intense AR reactivity was seen in both stroma and epithelial regions in the TRAMP 22 group. Intense IGFR-1 and MMP-9 stromal immunolabeling was identified in both TRAMP groups PMID: 27036326
  37. MMP-9 inhibition results from a joint contribution between the components of the extract from Chilean Propolis and Pinocembrin PMID: 27119082
  38. The results suggested that the relieving effect of KGLY against LPS-induced ALI might be partially due to suppression of oxidative stress and inflammatory response, inhibition of TLR4-mediated NF-kappaB activation, and down-regulation of MMP9 expression, indicating it may be a potential therapeutic agent for ALI. PMID: 27036629
  39. peritubular myoid cells exhibit part of the cross-talk which takes place between tumor and seminiferous peritubular myoid cells to regulate matrix-metalloproteinase 9 expression, emphasizing the important role of TNF-a as a crucial signaling component. PMID: 26711538
  40. Matrix metalloproteinase-9 deletion rescues auditory evoked potential habituation deficit in a mouse model of Fragile X Syndrome. PMID: 26850918
  41. Rosuvastatin inhibits MMP-9 expression by upregulating miR-497 in HUVECs and apoE knockout mice, and the combination of rosuvastatin and probucol enhances this effect. PMID: 26502925
  42. data reveal a new cell-signaling role for MMP-9 through CD36 degradation to regulate macrophage phagocytosis and neutrophil apoptosis. PMID: 26578544
  43. Curcumin improves bone microarchitecture in glucocorticoid-induced secondary osteoporosis mice through the activation of microRNA-365 and suppression MMP-9 activity. PMID: 26884838
  44. RhoA-PLD1 signaling is involved in acidic extracellular pH-induced matrix metalloproteinase-9 in mouse metastatic B16-BL6 melanoma cells PMID: 26782071
  45. Our results suggest that MMP-9 deletion may reduce age-related myocardial stiffness, which may explain improved cardiac function in MMP-9 null animals. PMID: 26415707
  46. In diabetic retinopathy transcription of MMP-9 is regulated by AP-1 binding at both, proximal and distal sites of its promoter, and acetylation of c-Jun and c-Fos subunits is important in its regulation. PMID: 26599598
  47. Found hyperexpression of exogenous hypoxia response element-matrix metalloproteinase-9 under the control of hypoxia, and its expression was mainly located in neurons and astrocytes without aggravation of blood brain barrier damage. PMID: 25975730
  48. CXCR4 inhibitor attenuates allergen-induced lung inflammation by down-regulating MMP-9 and ERK1/2 expression. PMID: 26261552
  49. MMP-9 mediates thrombus-induced loss of vein wall compliance by increasing stiffness of the extracellular matrix and collagen-elastin fibers during thrombus resolution. PMID: 26406902
  50. the principal H3NT protease of osteoclastogenesis is matrix metalloproteinase 9 (MMP-9 PMID: 26744418

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Peptidase M10A family
Database Links

KEGG: mmu:17395

STRING: 10090.ENSMUSP00000017881

UniGene: Mm.4406

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