Product Details

Purity

>85% (SDS-PAGE)

Target Names

Prkcg

Uniprot No.

P63318

Alternative Names

Prkcg; Pkcc; Pkcg; PrkccProtein kinase C gamma type; PKC-gamma; EC 2.7.11.13

Species

Mus musculus (Mouse)

Expression Region

1-697

Target Protein Sequence

MAGLGPGGGD SEGGPRPLFC RKGALRQKVV HEVKSHKFTA RFFKQPTFCS HCTDFIWGIG KQGLQCQVCS FVVHRRCHEF VTFECPGAGK GPQTDDPRNK HKFRLHSYSS PTFCDHCGSL LYGLVHQGMK CSCCEMNVHR RCVRSVPSLC GVDHTERRGR LQLEIRAPTS DEIHITVGEA RNLIPMDPNG LSDPYVKLKL IPDPRNLTKQ KTKTVKATLN PVWNETFVFN LKPGDVERRL SVEVWDWDRT SRNDFMGAMS FGVSELLKAP VDGWYKLLNQ EEGEYYNVPV ADADNCSLLQ KFEACNYPLE LYERVRMGPS SSPIPSPSPS PTDSKRCFFG ASPGRLHISD FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS GPDGEPTIRA HGFFRWIDWE RLERLEIAPP FRPRPCGRSG ENFDKFFTRA APALTPPDRL VLASIDQADF QGFTYVNPDF VHPDARSPTS PVPVPVM

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contribute to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock.

Gene References into Functions

cPKC&-gamma modulated sequential reactivation of mTOR inhibited autophagic flux in neurons exposed to OGD/R, which may provide endogenous interventional strategies for stroke, especially ischemia/reperfusion injury PMID: 29734780
PKCgamma-mediated down-regulation of UCHL1 alleviates ischemic neuronal injuries by decreasing autophagy via ERK-mTOR pathway. PMID: 28726275
SUMOylation of EphB1 repressed activation of its downstream signaling molecule PKC-gamma, and consequently inhibited neuroblastoma tumorigenesis. PMID: 29550816
CA8 is a novel important regulator of Purkinje cell dendritic development and that its expression is controlled by PKCgamma activity. PMID: 26399641
These results suggested that cPKCgamma-modulated neuron-specific autophagy improves the neurological outcome of mice following ischemic stroke through the Akt-mTOR pathway, providing a potential therapeutic target for ischemic stroke. PMID: 27510769
Lysophosphatidylcholines prime polymorphonuclear neutrophil through Hck-dependent activation of PKCdelta, which stimulates PKCgamma, resulting in translocation of phosphorylated p47(phox). PMID: 27531930
Immunoblotting and RT-PCR results showed that NIHL increased the expression of PKCgamma but decreased that of GABABR1 and GABABR2 at both protein and mRNA levels in the CNC PMID: 25592416
PRKCgamma is critical for the localization of slit diaphragm components in the mouse kidney. PMID: 24700503
Therefore, we propose that PKCgamma positively modulates dopamine release through beta2PIX phosphorylation. PMID: 25009260
These findings indicate that the identity of the calcium-dependent PKCgamma that mediates PTP controls the mechanism and functional consequences of Posttetanic potentiation. PMID: 24794094
This study provided new evidence to support the possibility of the involvement of PKC-gamma in the actions of volatile anesthetics in mice brain. PMID: 23564155
demonstrate a role for the gamma isotype of protein kinase C (PKCgamma) in food-mediated entrainment of behavior and the molecular clock PMID: 23185022
transient receptor potential 3 which is also needed for mGluR1-dependent slow excitatory postsynaptic potentials and motor coordination and associates with PKCgamma PMID: 23115168
cPLA(2)-dependent AA release is required for VEGF-induced Src-PLD1-PKCgamma-mediated pathological retinal angiogenesis PMID: 21536681
reports multiple cPKCgamma-interacting proteins in HPC mouse brain and suggested that cPKCgamma signaling molecules, especially the cPKCgamma-synapsin pathway, might be responsible for HPC-induced neuroprotection against cerebral ischemic injuries of mice PMID: 21335048
the expression of transthyretin and protein kinase Cgamma were increased in the prefrontal cortex but not in the hippocampus of naltrexone-treated mice PMID: 21111029
a potential link between the increased PKCgamma expression and the age-related hearing loss PMID: 21056652
Spinal PKCgamma is likely involved in multiple adaptive responses following systemic morphine administration. PMID: 20359526
Congo red inhibits the aggregation of mutant PKCgamma that causes SCA14 by inhibiting oligomerization. PMID: 20938103
The N-methyl-d-aspartate (NMDA) receptor/PKCgamma signaling pathway may participate in the development of bone cancer pain. PMID: 20362561
trehalose counteracts various cellular dysfunctions that are triggered by mutant gammaPKC in both neuronal cell lines and primary cultured PCs by inhibiting oligomerization and aggregation of mutant gammaPKC. PMID: 20705605
phospholipase C-gamma1 is regulated by protein kinase A-dependent phosphorylation. PMID: 12123716
loss of the PKC gamma gene may protect the functional ORL-1 and mu-opioid receptors from degradation by phosphorylation in the mouse pons/medulla. PMID: 12359310
These results suggest that the PKC signal transduction pathway is critically involved in the facilitating action of clozapine on the NMDA-induced responses in pyramidal cells of the mPFC. PMID: 12699785
Male gamma-PKC null mutants exhibit increased sensitivity to activating doses of ethanol in contrast to previous findings. This reflects the pleiotropic effects of the gamma-PKC null mutation on the behavioral effects of ethanol. PMID: 14690876
Data provide direct evidence that PKC gamma is differentially regulated in the hippocampus of C57BL/6J and DBA/2J mice by acute stress. PMID: 15364414
The effect of chronic ethanol treatment on transthyretin expression was analyzed because gamma-PKC mutants do not develop tolerance to chronic ethanol treatment. PMID: 16767509
Lens oxidative stress treatment by H(2)O(2) significantly induced lens opacification (cataract) in the PKCgamma knockout mice when compared to controls. PMID: 17050852
These results suggest that the activation of spinal PKCgamma associated with the activation of ascending pain transmission may be an important factor in chronic pain-like hyperalgesia with changes in emotionality. PMID: 18242849
PKCgamma interneurons are activated by myelinated afferents responding to innocuous stimuli. Injury-induced mechanical allodynia may be transmitted through PKCgamma interneurons and non-nociceptive, VGLUT1-expressing myelinated afferents. PMID: 18685019
In pilocarpine-induced status epilepticus, PKCgamma induction at 1 day in pyramidal neurons, or induction in interneurons at the oriens/alveus border at 2h may be specifically linked to the activation of mGluR5. PMID: 18774262
Spatial experience & training result in altered activation of PKCgamma. PKCgamma activation in the CA1 region participates in postsynaptic plasticity associated with spatial experiences & learning. PMID: 19466636
the 5-HT(2B) receptor-induced proliferative response of the interstitial cells of Cajal is through phospholipase C, [Ca(2+)](i), and PKCgamma, implicating this PKC isoform in the regulation of cellular proliferation PMID: 19531484

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, dendrite.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Tissue Specificity

Expressed in the cerebellum, cerebral cortex and hippocampus (at protein level). Highly expressed in Purkinje cells.

Database Links

KEGG: mmu:18752

STRING: 10090.ENSMUSP00000097874

UniGene: Mm.7980

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Recombinant Mouse Protein kinase C gamma type (Prkcg)

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