Recombinant Pseudomonas aeruginosa Probable D-amino acid oxidase PA4548 (PA4548)

Code CSB-YP335851EZX
MSDS
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Source Yeast
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Code CSB-EP335851EZX
MSDS
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Source E.coli
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Code CSB-EP335851EZX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335851EZX
MSDS
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Source Baculovirus
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Code CSB-MP335851EZX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
thiO
Uniprot No.
Alternative Names
thiO; PA4548Glycine oxidase; GO; EC 1.4.3.19
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-364
Target Protein Sequence
MSERVVVVGA GVIGLLTARE LALAGLRVTL VERGESGREA SWAGGGIVSP LYPWRYSPAV TALAHWSQDF YPALGQRLLD ETGLDPEVHT VGLYWLDLDD QTEALQWARK HTRPLKEVPI EEAYAAVPGL GAGFQRAVYM SGVANVRNPR LARSLRASLQ QFANLELHEQ TEVRGWLRDG DRVVGVATSR GEIRGDKVLL AAGAWSGELL KPLGLELPVV PVKGQMILYK CAADFLPRMV LAKGRYAIPR RDGHILIGST LEHSGFDKTP TDEAQESLRA SAAELLPELA DMQPVAHWAG LRPGSPEGIP YIGPVPGFDG LWLNTGHYRN GLVLAPASCR LLADLMSGRE PIIDPAPYAP AGRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the oxidation of glycine, leading to glyoxyl imine and hydrogen peroxide as primary products; glyoxyl imine is used for the biosynthesis of the thiazole ring of thiamine. Otherwise, glyoxyl imine is spontaneously hydrolyzed in water to produce glyoxylate and ammonia. Can also use sarcosine (N-methylglycine) as substrate.
Protein Families
DAO family, ThiO subfamily
Database Links

KEGG: pae:PA4548

STRING: 208964.PA4548

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