Recombinant Rat Serine protease 1 (Prss1)

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Code CSB-EP018811RA
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018811RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Prss1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018811RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Prss1.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Prss1
Uniprot No.
Research Area
Others
Alternative Names
Prss1; Try1; Anionic trypsin-1; EC 3.4.21.4; Anionic trypsin I; Pretrypsinogen I; Serine protease 1
Species
Rattus norvegicus (Rat)
Source
E.coli
Expression Region
24-246aa
Target Protein Sequence
IVGGYTCPEHSVPYQVSLNSGYHFCGGSLINDQWVVSAAHCYKSRIQVRLGEHNINVLEGDEQFINAAKIIKHPNYSSWTLNNDIMLIKLSSPVKLNARVAPVALPSACAPAGTQCLISGWGNTLSNGVNNPDLLQCVDAPVLSQADCEAAYPGEITSSMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCALPDNPGVYTKVCNFVGWIQDTIAAN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
39.6kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Just like other recombinant proteins, the production of this recombinant Rat Prss1 protein began with appropriate cDNA and PCR methods, and then the Prss1 expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Rat Prss1 protein. N-terminal 6xHis-SUMO tag was used in the process. And we finally get the protein of interest with purity of 90%+.

Prss1 is a gene providing an instruction of making a protein named anionic trypsin-1 (also called anionic trypsin I, pretrypsinogen I and serine protease 1 in rat. This protein, the precursor of the major form of trypsin, has serine-type endopeptidase activity and can bind with metal ion. It is involved in multiple biological process, including digestion, proteolysis,and response to caffeine, nicotine, nutrient and organic substance. Recombinant prss1 from CUSABIO is full length of mature protein, which has been removed signal peptide and propeptide.

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Target Background

Gene References into Functions
  1. ectopic anionic trypsin I in rat lungs induced by virus infection may trigger virus spread in rat lungs PMID: 12027901
Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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