Recombinant Rat Eukaryotic translation initiation factor 4E-binding protein 1(Eif4ebp1)

Code CSB-YP730796RA
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Source Yeast
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Code CSB-EP730796RA
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Source E.coli
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Code CSB-EP730796RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP730796RA
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Source Baculovirus
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Code CSB-MP730796RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Eif4ebp1
Uniprot No. Q62622
Alternative Names Eif4ebp1Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Species Rattus norvegicus (Rat)
Expression Region 2-117
Target Protein Sequence SAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPTIPGV TSPTSDEPPM QASQSHLHSS PEDKRAGGEE SQFEMDI
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Gene References into Functions
  1. FGF signaling differentially impacts protein synthesis through either stimulation or repression, in a cell-type-dependent manner, with 4E-BP1 being a key player. PMID: 27313212
  2. Phosphorylation of 4EBP1 is suppressed following treatment with everolimus in combination with radiation. PMID: 25200811
  3. peptides that encompass the Raptor cross-linking region of 4E-BP1 inhibit cross-linking and interaction of 4E-BP1 with Raptor. PMID: 24403073
  4. New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. PMID: 20736160
  5. eIF4E is rate-limiting for cell cycle progression before it regulates cell growth PMID: 14607835
  6. phosphorylation of 4E-BP1 is regulated by L-arginine through the mTOR signaling pathway PMID: 15010853
  7. eukaryotic initiation factor 4E-binding protein 1 gene transcription is regulated by polyamines PMID: 15351722
  8. Lipopolysaccharide did not prevent the ability of pharmacological levels of IGF-I to phosphorylate 4E-BP1, S6K1, mTOR or alter the availability of eIF4E. PMID: 15389631
  9. Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on 4E-BP1 PMID: 15767663
  10. The oxidative stress generated by H2O2 in NGF-differentiated PC12 cells, 4E-BP1 and eIF4E phosphorylated levels decrease significantly, and an enhancement of the association of 4E-BP1 to eIF4E, which in turn decreases eIF4F formation is observed. PMID: 15907373
  11. AMPK activation attenuates 4E-BP1 response to high-frequency electrically stimulated skeletal muscle contractions. PMID: 18187610
  12. eIF4G integrity and hyperphosphorylated 4E-BP1 are needed to ensure appropriate translation in neurons PMID: 18215131

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Protein Families EIF4E-binding protein family
Tissue Specificity Expressed in all tissues examined; highest levels in fat and skeletal tissue, lowest levels in kidney.
Database Links

KEGG: rno:116636

STRING: 10116.ENSRNOP00000016885

UniGene: Rn.11161

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