Recombinant Rat Guanine nucleotide-binding protein G (i) subunit alpha-2 (Gnai2)

Code CSB-YP009589RA
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Source Yeast
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Code CSB-EP009589RA
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Source E.coli
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Code CSB-EP009589RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009589RA
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Source Baculovirus
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Code CSB-MP009589RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gnai2
Uniprot No.
Alternative Names
Gnai2; Gnai-2Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein
Species
Rattus norvegicus (Rat)
Expression Region
2-355
Target Protein Sequence
GCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR ADDARQLFAL SCAAEEQGML PEDLSGVIRR LWADHGVQAC FGRSREYQLN DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSPLTICFPE YTGANKYDEA ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK DCGLF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Gene References into Functions
  1. paraventricular nucleus Galphai(2) protein pathways play an endogenous role in maintaining fluid and electrolyte balance by controlling the influence the sympathetic nervous system has on the renal handling of sodium PMID: 22459149
  2. endoplasmic reticulum-resident G(i2) protein is an important negative regulator of vesicular transport at the early stage of vesicle formation before coat protein assembly PMID: 21480366
  3. involvement of Gi2 in activation of extracellular signal-regulated kinases in hepatocytes PMID: 11495629
  4. Gi2 has a role in regulating mitogenic activity in the neural progenitor cells of developing brain PMID: 15272018
  5. Alternatively spliced variant G alpha i2 is widely expressed in rat brain regions known to play a crucial role in brain function. PMID: 15312896
  6. heterologous desensitization in INS-1 cells may be mediated by Galphai2 changes but depend on the cAMP/PKA signalling pathway probably distant form the Galphai2 protein PMID: 15376236
  7. This novel alternatively spliced isoform of Galphai2 putatively encodes a truncated protein that could serve unique roles in the physiology of the vestibular neuroepithelium. PMID: 15950765
  8. Galphai2 has an essential regulatory role in ciliary function and cerebral spinal fluid homeostasis. PMID: 17430589
  9. results indicate that the upregulation of Gi2 in ischemic myocardium appears to be protective in inducing cell survival pathways to prevent myocyte death in response to ischemia/reperfusion injury PMID: 18316484
  10. Highest protein level was observed at the age of P0. There was also a trend of decline in the sGalphai2 protein from P0 to P90 in brain PMID: 18472243
  11. change of G[alpha]i2, G[alpha]q mRNA, and their proteins in severe acute pancreatitis PMID: 18665079
  12. role of Galpha(i2) in development of insulin resistance PMID: 18981538
  13. Overexpression of Galpha(i2), PDE2a, and RGS2 down-regulates the beta-adrenergic signaling pathway, thus contributing to the pathogenesis of cirrhotic cardiomyopathy. PMID: 19003918

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Subcellular Location
Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Membrane; Lipid-anchor.
Protein Families
G-alpha family, G(i/o/t/z) subfamily
Database Links
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