Recombinant Rat NF-kappa-B inhibitor alpha(Nfkbia)

Code CSB-YP733969RA
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Source Yeast
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Code CSB-EP733969RA
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Source E.coli
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Code CSB-EP733969RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733969RA
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Source Baculovirus
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Code CSB-MP733969RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Nfkbia
Uniprot No. Q63746
Alternative Names Nfkbia; Ikba; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; RL/IF-1
Species Rattus norvegicus (Rat)
Expression Region 1-314
Target Protein Sequence MFQPAGHGQD WAMEGPRDGL KKERLVDDRH DSGLDSMKDE DYEQMVKELR EIRLQPQEAP LAAEPWKQQL TEDGDSFLHL AIIHEEKTLT MEVIGQVKGD LAFLNFQNNL QQTPLHLAVI TNQPGIAEAL LKAGCDPELR DFRGNTPLHL ACEQGCLASV AVLTQTCTPQ HLHSVLQATN YNGHTCLHLA SIHGYLGIVE HLVTLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQTL PESEDEESYD TESEFTEDEL PYDDCVFGGQ RLTL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (By similarity).
Gene References into Functions
  1. Myricetin Possesses Potential Protective Effects on Diabetic Cardiomyopathy through Inhibiting IkappaBalpha/NFkappaB and Enhancing Nrf2/HO-1. PMID: 29147465
  2. Data suggest that expression and phosphorylation of Nfkbia in adipose tissue can be altered by dietary factors; here, obese rats treated with anti-obesity dietary supplement taurine exhibit increased expression of Nfkbia and decreased ratio of phosphorylated/non-phosphorylated Nfkbia intra-abdominal adipose tissue (and decreased adiposity). PMID: 26621632
  3. These results indicated that miR-199b attenuated acute spinal cord injury at least partly through IKKbeta-NF-kappaB signaling pathway and affecting the function of microglia. PMID: 27693495
  4. enhanced miR-381a-3p expression contributed to the injury of osteoarthritis mainly by inhibiting the expression of IkappaBalpha. PMID: 27312547
  5. Thus, the inhibition of the IKK complex might be an effective strategy for the prevention of multiple organ failure associated with hemorrhagic shock PMID: 26101953
  6. TFPI inhibited MCP-3 expression induced by TNF-alpha; this effect may be propagated through the NF-kappaB/IkappaB-alpha pathway. PMID: 23374966
  7. NF-kappaB signaling through IkappaBalpha degradation results in manganese superoxide dismutase (MnSOD) upregulation and preserved cell growth, whereas NF-kappaB signaling through IkappaBbeta degradation mediates apoptosis and P-selectin expression. PMID: 23418625
  8. Data from an obesity model suggest that as duration of plasma free fatty acid elevation increases, the IKKB pathway in liver becomes inactivated leading to insulin resistance; levels of phospho-IkBa and IkBa in liver were monitored in this study. PMID: 23328071
  9. a role for IKKalpha in accelerating the differentiation of human NPCs and identify IKKalpha as a potential regulator of MeCP2 function and BDNF expression PMID: 22848609
  10. These results suggest the existence of a reservoir of monoubiquitylated IkappaBalpha resistant to TNFalpha-induced proteolysis. PMID: 22022389
  11. Redox regulation of IkappaB-alpha nuclear transport and activation in rat PMID: 12200125
  12. basal IkappaBalpha expression (and as a consequence NF-kappaB activity) is under the control of the various components of the CBF1/Notch signal transduction pathway PMID: 12700242
  13. IKBA is degraded by HSP27 through the 26s proteasome PMID: 12897149
  14. IKBA has a role in tumor necrosis factor-stimulated insulin resistance in skeletal muscle PMID: 14764603
  15. anti-inflammatory effects of SP-A on LPS-challenged alveolar macrophages are associated with a SP-A-mediated direct modulation of the IkappaB-alpha turnover PMID: 15308505
  16. diet-induced obesity and acute hyperlipidemia reduce IkappaBalpha levels in rat skeletal muscle in a fiber-type-dependent manner PMID: 16081881
  17. the mechanism preventing septic activation of NF-kappaB is that oversynthesized HSP72 forms a complex with NF-kappaB/I-kappaB, thus inhibiting nuclear translocation of NF-kappaB PMID: 16135962
  18. Results describe the coincident enrichment of phosphorylated IkappaBalpha, activated IKK, and phosphorylated p65 in the axon initial segment of neurons. PMID: 16875840
  19. IkappaBalpha degradation is required for the unloading-induced decrease in fiber size, the increase in protein ubiquitination, activation of NF-kappaB signaling, and the expression of specific atrophy genes PMID: 16928772
  20. data suggest a new mechanism by which calpain 3 activation is able to regulate the IkappaBalpha/NF-kappaB pathway and thus neurodegenerative processes PMID: 16938483
  21. indicate that anti-inflammatory macrophage activation via IkappaB-alpha by Surfactant protein A critically depends on protein kinase C zeta activity PMID: 17878344
  22. alteration of NF-kappaB and I-kappa B might play an important role in aberrant proliferation within the intima and vascular remodeling following vascular injury PMID: 18278452

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Subcellular Location Cytoplasm, Nucleus
Protein Families NF-kappa-B inhibitor family
Database Links

KEGG: rno:25493

STRING: 10116.ENSRNOP00000009894

UniGene: Rn.12550

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