Product Details

Purity

>85% (SDS-PAGE)

Target Names

Prkcg

Uniprot No.

P63319

Alternative Names

Prkcg; Pkcc; Pkcg; PrkccProtein kinase C gamma type; PKC-gamma; EC 2.7.11.13

Species

Rattus norvegicus (Rat)

Expression Region

1-697

Target Protein Sequence

MAGLGPGGGD SEGGPRPLFC RKGALRQKVV HEVKSHKFTA RFFKQPTFCS HCTDFIWGIG KQGLQCQVCS FVVHRRCHEF VTFECPGAGK GPQTDDPRNK HKFRLHSYSS PTFCDHCGSL LYGLVHQGMK CSCCEMNVHR RCVRSVPSLC GVDHTERRGR LQLEIRAPTS DEIHITVGEA RNLIPMDPNG LSDPYVKLKL IPDPRNLTKQ KTKTVKATLN PVWNETFVFN LKPGDVERRL SVEVWDWDRT SRNDFMGAMS FGVSELLKAP VDGWYKLLNQ EEGEYYNVPV ADADNCSLLQ KFEACNYPLE LYERVRMGPS SSPIPSPSPS PTDSKRCFFG ASPGRLHISD FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS GPDGEPTIRA HGFFRWIDWE RLERLEIAPP FRPRPCGRSG ENFDKFFTRA APALTPPDRL VLASIDQADF QGFTYVNPDF VHPDARSPTS PVPVPVM

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component ARNTL/BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock.

Gene References into Functions

acute stress causes the enhancement of acid-sensing ion channels function by activating PKC signaling pathway, which leads to potentiated learning and memory. PMID: 29555470
It could be activated by hyperglycemia induced inflammatory reaction. PMID: 29121795
In summary, ionomycin-promoted exocytosis of GLUT4 is partly reversed by siPKCtheta;, whereas ionomycin-inhibited endocytosis of GLUT4 requires both siPKCalpha and siPKCtheta;. PKCalpha and PKCtheta; contribute to ionomycin-induced phosphorylation of AS160 and TBC1D1. Rab13 is required for ionomycin-regulated GLUT4 exocytosis. PMID: 29247648
PKC phosphorylates cardiac troponin I (cTnI) S23/24, S43/45 and T144 to fine tune myocyte function. PMID: 28587770
In a model of cancer-induced bone pain, protein kinase C gamma is up-regulated in the spine. PMID: 27848062
the intact subepidermal nerve fibers persistently increase PKCg activation and mTOR-dependent PKCg synthesis participate in the initiation and maintenance of mechanical hypersensitivity in spared nerve injury. PMID: 27296621
This study demonistrated that the PKCgamma interneuron within the rat medullary dorsal horn. PMID: 25961142
aPKC may be a convergence point and a rate limit step in the pathway by which, insulin and contraction, increase glucose uptake in skeletal muscle. PMID: 25529429
Therefore, we propose that PKCgamma positively modulates dopamine release through beta2PIX phosphorylation. PMID: 25009260
These results demonstrate that PKCdelta, NADPH oxidase, and oxidative stress play a causal role in FFA-induced hepatic insulin resistance. PMID: 24824652
A novel mechanism of nociceptive-induced remote conditioning, involving gammaPKC, was identified. PMID: 23982492
PKC may play an important role in neuronal differentiation and synaptogenesis via stimulating HuD-mediated mRNA stability and inhibiting CARM1 in hippocampal neurons PMID: 22736542
Pioglitazone can ameliorate neonatal rat cardiomyocyte injury induced by hypoxia/reoxygenation partially by activating PPARgamma and does not increase the expression of PKC in the cells. PMID: 22126757
study to elucidate the mechanisms that PKCgamma regulates neuropathic pain (NP), and detect proteins that are associated with the function of PKCgamma in NP; study used the 2DGE-baed comparative proteomics in combination with gene silencing technique to identify novel protein targets that are involved in the PKCgamma signal pathway in NP PMID: 22284620
During morphine dependence, but not naloxone-precipitated withdrawal, PKC gamma in the spinal cord translocated from cytosol to membrane fraction. PMID: 15830100
Data show that that PKCbetaI and PKCgamma expression decreased significantly both in mRNA and protein levels in hypothyroid group compared with the normal controls. PMID: 21424759
PKCgamma regulates ethanol-induced alterations in gamma-aminobutyric acid (GABA)A receptor alpha4 subunit expression in cerebral cortical neurons. PMID: 21155805
The cardioprotective effect exerted by PAF pre-treatment involves activation of PKC and PI3K in post-ischaemic phases and might be mediated by the prevention of mPTP opening in reperfusion via GSK-3beta inactivation PMID: 19432589
Individual differences in spatial memory among aged rats are related to hippocampal PKCgamma immunoreactivity. PMID: 12000125
PKC is translocated to the cell membranes, particularly synaptic membranes, where it may modulate cellular function PMID: 14688616
chronological expression of PKC isoforms in dental follicle follows different profiles; expression level of PKC-gamma was relatively steady in the postnatal days; study suggests that PKC isoforms may be involved in the regulation of tooth eruption PMID: 15763930
PKC-inhibition with ruboxistaurin led to attenuation in both the pathological fibrosis and impaired cardiac function that follow experimental MI, suggesting a possible role for this agent in preventing post-infarction heart failure. PMID: 15878171
Simultaneous inhibition of PI3K and PKC can induce GSK-3 overactivation, and further strengthen and prolong the Alzheimer-like tau hyperphosphorylation in N2a cells PMID: 15880264
PKC-gamma interacted with P2X(7) receptor complex and positively regulated the receptor-mediated Ca(2+) signalling. PMID: 15985361
These results demonstrate that the developmental decrease in PKC activity and expression in the rat myocardium is not linear and that subcellular localization of the enzyme exhibits isoform-specific day-by-day changes during the early postnatal period. PMID: 16043888
The results suggest that ethanol age-dependently alters sensitivity to mechanical and thermal stimuli via specific protein kinase C isozymes. These results begin to ascertain the mechanisms that produce abnormal pain after alcohol exposure. PMID: 16084468
protein kinase C-gamma mutant rats AS/AGU show an initial dysfunction in transmitter release leading to cell death PMID: 16324113
These results indicate that conventional and novel PKCs attenuate lipolysis mediated by beta-adrenergic receptors, probably through inhibition of the beta1-adrenergic receptor system. PMID: 16500613
Together, the dual activation requirement for PKCgamma provides a plausible molecular interpretation for different synergistic contributions of mGluRs to long-term potentiation and other synaptic plasticity processes. PMID: 16571747
The functional interaction between three bona fide regulators of neurotransmitter release, such as GABAB receptor (GBR), NEM sensitive fusion (NSF) and Protein Kinase C, could shed new light on the modulation of presynaptic GBR action. PMID: 16724110
localization and activation of the functionally correlated kinases, gammaPKC and DGKgamma, are spatio-temporally orchestrated by their direct association and phosphorylation, contributing to subtype-specific regulation of DGKgamma and DAG signaling PMID: 16905533
the number of PKCgamma positive gracile cells that expressed also c-fos increased after injury, indicating an alteration in the sensory activation pattern in these neurones after injury PMID: 18387748
These findings provide the first evidence that different PKC isozymes exert class-specific opposing effects on cardiac L-type Ca(2+) channel activity in L6 myoblasts. PMID: 18456322
The involvement of protein kinase C (PKC) in progesterone-induced rapid signaling in the ventromedial nucleus of the hypothalamus (VMN) and preoptic area (POA) of the rat brain. PMID: 18617608
PKC and MLCK pathways important in coronary endothelial dysfunction elicited by ischemia/reperfusion injury and cytokine release PMID: 18621396
PKCgamma interneurons are activated by myelinated afferents responding to innocuous stimuli. Injury-induced mechanical allodynia may be transmitted through PKCgamma interneurons and non-nociceptive, VGLUT1-expressing myelinated afferents. PMID: 18685019
Immunohistochemistry showed positive staining for PKCgamma in smooth muscle cells of rat posterior cerebral arteries; results suggest distinct roles of PKC isoforms in myogenic constriction: conventional PKC mediates Ca(2+) sensitization PMID: 19057126

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Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, dendrite.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Database Links

KEGG: rno:24681

STRING: 10116.ENSRNOP00000019825

UniGene: Rn.9747

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Recombinant Rat Protein kinase C gamma type (Prkcg)

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