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The generation of the ANGPT2 recombinant monoclonal antibody involves a precise and thorough process to ensure its exceptional quality and specificity. It begins by isolating B cells from the spleen of an immunized animal, where the recombinant human ANGPT2 protein serves as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The ANGPT2 antibody genes are amplified using specific primers designed for the antibody constant regions and then inserted into an expression vector. Through transfection, the vector is introduced into host cells, enabling the production of the ANGPT2 recombinant monoclonal antibody. Following a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for a variety of applications. The antibody's specificity and functionality have been tested in ELISA for detecting human ANGPT2 protein. This meticulous production process ensures the production of a reliable and effective ANGPT2 recombinant monoclonal antibody, crucial for diverse ANGPT2-related research.
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