Code | CSB-RA010429A16acHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:50-1:200 |
Producing the acetyl-histone H4 (K16) recombinant monoclonal antibody is a methodical process that commences with the cloning of genes encoding the HIST1H4A antibody, covering both heavy and light chains. These cloned genes are integrated into expression vectors, which are introduced into host cells through transfection. Subsequently, the host cells are entrusted with antibody production and secretion in a suitable medium. The purified antibody, obtained through affinity chromatography, undergoes comprehensive testing to assess its functionality across diverse applications such as ELISA, WB, ICC, IF, and FC, all tailored for detecting the human and mouse HIST1H4A proteins acetylated at K16.
Histone H4 is a core histone involved in chromatin structure and gene regulation. Acetylation of histone H4 at lysine 16 (H4K16) primarily functions in chromatin decondensation, transcriptional activation, DNA repair, epigenetic signaling, cellular memory, and coordinated gene regulation.
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