Acetyl-Histone H4 (K5) Antibody

Datasheet
Code CSB-RA010429A05acHU
Size US$350
Uniprot No. P62805
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,MCF-7 whole cell lysate,293 whole cell lysate,HepG2 whole cell lysate
    All lanes:Acetyl-Histone H4 (K5) antibody at 1.05μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 11 KDa
    Observed band size: 11 KDa

  • Immunocytochemistry analysis of CSB-RA010429A05acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010429A05acHU at 1:65,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide
Species Reactivity Human
Tested Applications ELISA, WB, ICC, IF; Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:30-1:200
Relevance Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Histone H4, HIST1H4A, H4/A, H4FA, AND, HIST1H4B, H4/I, H4FI, AND, HIST1H4C, H4/G, H4FG, AND, HIST1H4D, H4/B, H4FB, AND, HIST1H4E, H4/J, H4FJ, AND, HIST1H4F, H4/C, H4FC, AND, HIST1H4H, H4/H, H4FH, AND, HIST1H4I, H4/M, H4FM, AND, HIST1H4J, H4/E, H4FE, AND, HIST1H4K, H4/D, H4FD, AND, HIST1H4L, H4/K, H4FK, AND, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, AND, HIST2H4B, H4/O, H4FO, AND, HIST4H4
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Epigenetics and Nuclear Signaling
Gene Names HIST1H4A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H4 family
Database Links

HGNC: 4781

OMIM: 142750

KEGG: hsa:121504

STRING: 9606.ENSP00000367034

UniGene: Hs.143080

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