Code | CSB-RA303909A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
FC | 1:20-1:200 |
The C3 recombinant monoclonal antibody can be utilized to identify human C3 protein in ELISA, WB, IHC, and FC applications. It is created using recombinant DNA technology. To synthesize the C3 monoclonal antibody gene, the cDNA of the C3 antibody-producing hybridomas is sequenced. The hybridomas are generated by fusing myeloma cells with B cells obtained from animals that were immunized with a synthesized peptide derived from human C3. The synthesized gene is cloned into a vector and then transfected into cells for cultivation. Following cultivation, the resulting C3 recombinant monoclonal antibody is purified through affinity chromatography from the cell culture supernatant.
Complement C3 is a key component of the complement system, which helps to identify and destroy foreign cells, such as bacteria and viruses or damaged cells through opsonization and the formation of the membrane attack complex (MAC). The activation of complement C3 occurs through several different pathways, including the classical pathway, the alternative pathway, and the lectin pathway. Once activated, C3 is cleaved into two fragments, C3a and C3b.
There are currently no reviews for this product.