Code | CSB-RA699863A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The C3 recombinant monoclonal antibody undergoes four production steps: (1) sequencing the C3 monoclonal antibody gene, (2) cloning the gene into a plasmid vector, (3) transfecting the recombinant vector into a host cell line, and (4) purifying the antibody from the cell culture supernatant using affinity chromatography. The C3 monoclonal antibody is derived from C3 antibody-producing hybridomas, and a synthesized peptide from human C3 serves as the immunogen during its production. The purified C3 recombinant monoclonal antibody is characterized and tested and recommended for use in detecting human C3 protein through ELISA and IHC applications.
Complement C3 is a protein that plays a central role in the complement system, which is a part of the innate immune system. Complement C3 is synthesized in the liver and is present in the blood plasma. When activated, it can be cleaved into smaller fragments (C3a and C3b) that mediate a variety of immune responses. It executes a critical role in the recognition and elimination of pathogens and the modulation of the immune response.
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