Code | CSB-RA003996A0HU |
Size | US$210 |
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The synthesis of the C5AR1 recombinant monoclonal antibody involves a meticulous process to ensure its exceptional quality and specificity. It begins by isolating B cells from an immunized animal using the recombinant human C5AR1 protein as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The C5AR1 antibody genes are then amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. Through transfection, the vector is introduced into host cells, allowing for the production of the C5AR1 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and subjected to purification using affinity chromatography, resulting in a highly purified form suitable for various applications. CUSABIO conducts ELISA to validate the antibody's specificity and functionality in detecting human C5AR1 protein.
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