CA9 Antibody

Datasheet
Code CSB-RA614990A0HU
Size US$350
Uniprot No. Q16790
Image
  • IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen A synthesized peptide derived from human CA9
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:50-1:200
Relevance Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9, G250, MN
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cardiovascular
Gene Names CA9
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Subcellular Location Nucleus, Nucleus, nucleolus, Cell membrane, Single-pass type I membrane protein, Cell projection, microvillus membrane, Single-pass type I membrane protein
Protein Families Alpha-carbonic anhydrase family
Tissue Specificity Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Database Links

HGNC: 1383

OMIM: 603179

KEGG: hsa:768

STRING: 9606.ENSP00000367608

UniGene: Hs.63287

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