Carbonic anhydrase IX (CA9/CAIX), also known as MN protein or G250 antigen, is a transmembrane zinc metalloenzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons. Primarily regulated by hypoxia-inducible factor 1-alpha (HIF-1α), it maintains intracellular pH homeostasis while acidifying the extracellular tumor microenvironment, thereby promoting cancer cell survival, invasion, and metastasis. CA9 is functionally linked to PI3K/AKT and ERK signaling pathways, with overexpression observed in clear cell renal cell carcinoma (ccRCC), triple-negative breast cancer, and colorectal adenocarcinoma. Its tumor-specific expression has positioned CA9 as both a diagnostic biomarker and therapeutic target. Clinical-stage agents include the small-molecule inhibitor SLC-0111 (Phase II) targeting CA9 enzymatic activity, and antibody-based therapies like ⁸⁹Zr-labeled girentuximab for PET imaging. CA9-targeted chimeric antigen receptor (CAR) T-cell therapies are under preclinical evaluation for ccRCC management.
Recombinant Human Carbonic anhydrase 9 (CA9), partial (CSB-EP614990HUc0)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP614990HUc0 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CA9.
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP614990HUc0 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CA9.
CA9 Recombinant Monoclonal Antibody (CSB-RA614990A0HU)
Validated Data
IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Human Carbonic Anhydrase 9(CA9) ELISA Kit (CSB-E13266h)
Validated Data
Code: CSB-E13266h
Size: 96T,5×96T,10×96T
Sensitivity: 7.81 pg/mL
Detection Range: 31.25 pg/mL-2000 pg/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following CA9 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CA9 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004380GA01HU | CA9 Antibody | Human | ELISA,WB |
CSB-PA614990GA01HU | CA9 Antibody | Human | ELISA,WB,IHC |
CSB-MA000234 | CA9 Monoclonal Antibody | Human | ELISA,WB,IP |
CSB-PA001096 | CA9 Antibody | Human | WB, ELISA |
CSB-PA179189 | CA9 Antibody | Human | ELISA,IHC |
CSB-PA550152 | CA9 Antibody | Human | ELISA,IHC |
CSB-RA614990A0HU | CA9 Recombinant Monoclonal Antibody | Human | ELISA, IHC |
CSB-PA614990ZA01HU | CA9 Antibody | Homo sapiens | ELISA, WB (ensure identification of antigen) |
CSB-PA614990ZA01HU | CA9 Antibody | Homo sapiens | ELISA, WB (ensure identification of antigen) |
CSB-PA614990LA01HU | CA9 Antibody | Human | ELISA, IHC |
CSB-PA614990LB01HU | CA9 Antibody, HRP conjugated | Human | ELISA |
CSB-PA614990LC01HU | CA9 Antibody, FITC conjugated | Human | |
CSB-PA614990LD01HU | CA9 Antibody, Biotin conjugated | Human | ELISA |
CA9 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP614990HU CSB-BP614990HU CSB-MP614990HU CSB-EP614990HU-B |
Recombinant Human Carbonic anhydrase 9 (CA9), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP614990HUc0 | Recombinant Human Carbonic anhydrase 9 (CA9), partial | E.coli |
CSB-EP614990HU | Recombinant Human Carbonic anhydrase 9 (CA9), partial | E.coli |
CA9 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP848704MO CSB-EP848704MO CSB-BP848704MO CSB-MP848704MO CSB-EP848704MO-B |
Recombinant Mouse Carbonic anhydrase 9 (Ca9), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CA9 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E13266h | Human Carbonic Anhydrase 9(CA9) ELISA Kit | serum, plasma, tissue homogenates | 7.81 pg/mL |