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CLDN18 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA005498A3HU
Size US$210
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Image
  • Untransfected NIH-3T3 cells (green line) and transfected Human CLDN18.1 NIH-3T3 stable cells (red line) were stained with anti-CLDN18 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
  • Untransfected KATO3 cells (green line) and transfected Human CLDN18.2 KATO3 stable cells (red line) were stained with anti-CLDN18 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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Product Details

Uniprot No.
P56856
Target Names
CLDN18
Alternative Names
Claudin 18 antibody; Claudin-18 antibody; CLD18_HUMAN antibody; CLDN18 antibody; SFTA5 antibody; SFTPJ antibody
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human CLDN18 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
9H5H3
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols
ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Claudin-18 is a member of the claudin family of tight junction proteins that plays a critical role in maintaining epithelial barrier integrity and regulating paracellular permeability. This protein exists as two splice variants with distinct tissue distributions: CLDN18.1 is predominantly expressed in lung alveolar epithelium, while CLDN18.2 shows highly restricted expression in differentiated gastric epithelial cells. The aberrant expression of CLDN18.2 in gastric and pancreatic cancers has made it an attractive target for therapeutic development and diagnostic research, positioning claudin-18 as a molecule of significant interest in oncology and epithelial biology studies.

This recombinant monoclonal antibody, clone 9H5H3, offers the reproducibility and sequence-defined consistency that demanding research applications require. Produced using recombinant technology and purified by affinity chromatography, each lot delivers reliable performance without the variability often encountered with traditional hybridoma-derived antibodies. The human IgG1 isotype format provides flexibility for detection systems utilizing anti-human Fc secondary reagents.

Flow cytometry validation demonstrates clear detection capability for both major claudin-18 isoforms. Testing on CLDN18.1-transfected NIH-3T3 cells and CLDN18.2-transfected KATO3 cells showed robust signal separation between transfected and untransfected populations, confirming specificity for the target protein. The antibody exhibits cross-species reactivity with human and mouse samples, supporting translational research workflows. Recommended dilutions for flow cytometry range from 1:50 to 1:200, with additional validation for ELISA applications.

For researchers investigating tight junction biology, epithelial barrier function, or claudin-18 as a therapeutic target, this antibody provides a dependable tool for cell surface detection and protein quantification studies.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. In this study, we found downregulation of miR-767-3p and upregulation of CLDN18 in lung adenocarcinoma tissue and cell lines. PMID: 29169410
  2. CDH17 and CLDN18 are useful target molecules. Their coupling can aid in the comprehensive detection and localization of gastric cancer metastases in vivo to overcome challenges associated with intratumoral heterogeneity. PMID: 27580354
  3. Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR/RAS/ERK pathway, contributing to cell proliferation and invasion. PMID: 28624624
  4. The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness PMID: 27215490
  5. suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
  6. Data suggest that claudin-18 suppresses the abnormal proliferation and motility of lung epithelial cells mediated by inhibition of phosphorylation of pyruvate dehydrogenase kinase isoform 1 (PDK1) and proto-oncogene protein c-akt (Akt). PMID: 26919807
  7. Human fetal lungs at 23-24 weeks gestational age, the highest-risk period for developing bronchopulmonary dysplasia, a disease of impaired alveolarization, had significantly lower CLDN18 expression relative to postnatal lungs. PMID: 24787463
  8. Evaluated expression of claudins in gastric cancer and determined their significance for patient outcome. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of gastric cancer tissues; 51.5% of gastric cancer tissues had reduced claudin-18. PMID: 24333468
  9. High levels of CLDN18 are associated with non-small-cell lung cancer. PMID: 24710653
  10. Downregulation of miR-1303 can inhibit proliferation, migration and invasion of gastric cancer cells by targeting CLDN18. PMID: 24647998
  11. Claudin-18 positivity is a specific phenotype that is characteristic of intestinal-type Mucinous borderline tumours of the ovary PMID: 23905715
  12. Down-regulation of claudin-18 is associated with the proliferative and invasive potential of gastric cancer. PMID: 24073219
  13. rate of CLDN18.2 positivity is high in pancreatic neoplasms whereby the expression is not limited to the primaries but is also maintained upon metastasis PMID: 23900716
  14. Claudin 10/18 are most commonly expressed in lung adenocarcinomas. Female patients and non-smokers express these claudins more commonly suggesting that they may play a part in the carcinogenesis of tobacco unrelated carcinoma. PMID: 22076167
  15. Claudin 18 (a marker for early carcinogenesis) is commonly expressed in precursor lesions of pancreatic ductal adenocarcinomas. Activation of the protein kinase C pathway might be involved in claudin 18 expression associated with carcinogenesis. PMID: 21832145
  16. Cldn18 is primarily regulated at the transcriptional level via specific protein kinase C signaling pathways and modified by DNA methylation PMID: 21381080
  17. These results suggest that CLDN18 may play an important role in biliary carcinogenesis. PMID: 21607649
  18. High claudin 18 is associated with intraductal papillary mucinous neoplasms of the pancreas. PMID: 21206985
  19. We revealed that claudin-18 expression correlates with poor survival in patients with colorectal cancer and is associated with the gastric phenotype. PMID: 20846265
  20. Loss of claudin expression may enhance the grade of malignancy of gastric cancer in vivo. PMID: 17459057
  21. We conclude that Cldn-18 is the dominant claudin in the TJ of SCE and propose that the change from a Cldn-18-deficient TJ in SqE to a Cldn-18-rich TJ in SCE contributes to the greater acid resistance of BE. PMID: 17932229
  22. Indicate that the PKC/MAPK/AP-1 dependent pathway regulates claudin-18a2 expression in gastric cells. PMID: 18032479
  23. Claudin 18 and annexin A8 are frequently highly overexpressed in infiltrating ductal adenocarcinomas. PMID: 18223320
  24. Claudin 18 staining can aid in diagnosis of gastrointestinal signet ring cell carcinoma. PMID: 18580680
  25. Increased expression of claudin-18 is associated with colitis. PMID: 18831034
  26. CLDN18.2 as a novel, highly attractive pan-cancer target for the antibody therapy of epithelial tumors. PMID: 19047087

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Subcellular Location
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families
Claudin family
Tissue Specificity
Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine c
Database Links

HGNC: 2039

OMIM: 609210

KEGG: hsa:51208

STRING: 9606.ENSP00000183605

UniGene: Hs.655324

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