Product Details

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 10 μg/mL can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005505MA1HU), the EC50 is 5.115-8.534 ng/mL.The VLPs (CSB-MP3838) is negative control.

Target Names

CLDN18.2

Uniprot No.

P56856

Alternative Names

(CLDN18.2)

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

1-261aa

Target Protein Sequence

MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV

Mol. Weight

52.3 kDa

Protein Length

Full Length of Isoform A2

Tag Info

C-terminal mCherry-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Datasheet & COA

Please contact us to get it.

Description

Claudin-18.2 presents a unique challenge for recombinant production because its four-transmembrane topology demands a native membrane context to preserve conformational epitopes critical for antibody recognition. This fluorescent VLP-displayed construct presents full-length human CLDN18.2 (aa 1–261, isoform A2) with a C-terminal mCherry tag, enabling direct visualization in flow cytometry-based antibody screening and cell-based binding assays. Functional ELISA confirms that immobilized CLDN18.2 VLPs bind an anti-CLDN18.2 recombinant antibody with an EC50 of 5.1–8.5 ng/mL, demonstrating preserved extracellular loop conformation suitable for antibody development and validation, therapeutic antibody benchmarking, and tumor metastasis research targeting CLDN18.2-positive cancers. The mammalian-expressed VLP format maintains native folding and membrane orientation while endotoxin levels below 1.0 EU/μg satisfy criteria appropriate for immune cell trafficking studies and integrin-ligand binding assays requiring low background activation.

Target Background

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Gene References into Functions

In this study, we found downregulation of miR-767-3p and upregulation of CLDN18 in lung adenocarcinoma tissue and cell lines. PMID: 29169410
CDH17 and CLDN18 are useful target molecules. Their coupling can aid in the comprehensive detection and localization of gastric cancer metastases in vivo to overcome challenges associated with intratumoral heterogeneity. PMID: 27580354
Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR/RAS/ERK pathway, contributing to cell proliferation and invasion. PMID: 28624624
The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness PMID: 27215490
suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
Data suggest that claudin-18 suppresses the abnormal proliferation and motility of lung epithelial cells mediated by inhibition of phosphorylation of pyruvate dehydrogenase kinase isoform 1 (PDK1) and proto-oncogene protein c-akt (Akt). PMID: 26919807
Human fetal lungs at 23-24 weeks gestational age, the highest-risk period for developing bronchopulmonary dysplasia, a disease of impaired alveolarization, had significantly lower CLDN18 expression relative to postnatal lungs. PMID: 24787463
Evaluated expression of claudins in gastric cancer and determined their significance for patient outcome. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of gastric cancer tissues; 51.5% of gastric cancer tissues had reduced claudin-18. PMID: 24333468
High levels of CLDN18 are associated with non-small-cell lung cancer. PMID: 24710653
Downregulation of miR-1303 can inhibit proliferation, migration and invasion of gastric cancer cells by targeting CLDN18. PMID: 24647998
Claudin-18 positivity is a specific phenotype that is characteristic of intestinal-type Mucinous borderline tumours of the ovary PMID: 23905715
Down-regulation of claudin-18 is associated with the proliferative and invasive potential of gastric cancer. PMID: 24073219
rate of CLDN18.2 positivity is high in pancreatic neoplasms whereby the expression is not limited to the primaries but is also maintained upon metastasis PMID: 23900716
Claudin 10/18 are most commonly expressed in lung adenocarcinomas. Female patients and non-smokers express these claudins more commonly suggesting that they may play a part in the carcinogenesis of tobacco unrelated carcinoma. PMID: 22076167
Claudin 18 (a marker for early carcinogenesis) is commonly expressed in precursor lesions of pancreatic ductal adenocarcinomas. Activation of the protein kinase C pathway might be involved in claudin 18 expression associated with carcinogenesis. PMID: 21832145
Cldn18 is primarily regulated at the transcriptional level via specific protein kinase C signaling pathways and modified by DNA methylation PMID: 21381080
These results suggest that CLDN18 may play an important role in biliary carcinogenesis. PMID: 21607649
High claudin 18 is associated with intraductal papillary mucinous neoplasms of the pancreas. PMID: 21206985
We revealed that claudin-18 expression correlates with poor survival in patients with colorectal cancer and is associated with the gastric phenotype. PMID: 20846265
Loss of claudin expression may enhance the grade of malignancy of gastric cancer in vivo. PMID: 17459057
We conclude that Cldn-18 is the dominant claudin in the TJ of SCE and propose that the change from a Cldn-18-deficient TJ in SqE to a Cldn-18-rich TJ in SCE contributes to the greater acid resistance of BE. PMID: 17932229
Indicate that the PKC/MAPK/AP-1 dependent pathway regulates claudin-18a2 expression in gastric cells. PMID: 18032479
Claudin 18 and annexin A8 are frequently highly overexpressed in infiltrating ductal adenocarcinomas. PMID: 18223320
Claudin 18 staining can aid in diagnosis of gastrointestinal signet ring cell carcinoma. PMID: 18580680
Increased expression of claudin-18 is associated with colitis. PMID: 18831034
CLDN18.2 as a novel, highly attractive pan-cancer target for the antibody therapy of epithelial tumors. PMID: 19047087

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Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Protein Families

Claudin family

Tissue Specificity

Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine c

Database Links

HGNC: 2039

OMIM: 609210

KEGG: hsa:51208

STRING: 9606.ENSP00000183605

UniGene: Hs.655324

Code	CSB-MP005498HU(A5)l5
Abbreviation	Recombinant Human CLDN18.2 protein, Fluorescent-VLPs (Active)
MSDS	MSDS Datasheet
Size	$456
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Image	Activity Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 at 10 μg/ml can bind Anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU), the EC50 is 5.115-8.534 ng/mL. The VLPs (CSB-MP3838) is negative control. Biological Activity Assay CSB-MP005498HU(A5)l5 is detected by cherry antibody.
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Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs, Fluorescent (Active)

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