CTPS1 Recombinant Monoclonal Antibody

Code CSB-RA548305A0HU
Size US$210
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Image
  • IHC image of CSB-RA548305A0HU diluted at 1:100 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No.
Target Names
CTPS1
Alternative Names
CTP synthase 1 (EC 6.3.4.2) (CTP synthetase 1) (UTP--ammonia ligase 1), CTPS1, CTPS
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human CTPS1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
13B6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO meticulously produced the CTPS1 recombinant monoclonal antibody through a well-defined process. B cells were isolated from the spleen of an immunized animal, with a synthesized peptide derived from human CTPS1 serving as the immunogen. RNA was extracted from the isolated B cells and converted into cDNA through reverse transcription. The gene encoding the CTPS1 antibody was then amplified using a degenerate primer and integrated into a vector. This recombinant vector was transfected into host cells to facilitate the efficient expression of the CTPS1 recombinant monoclonal antibodies. These antibodies were subsequently harvested from the cell culture supernatant and purified using affinity chromatography. The CTPS1 recombinant monoclonal antibody is suitable for use in ELISA and IHC and can react with human CTPS1 protein.

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Target Background

Function
This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
Gene References into Functions
  1. CTP synthase forms the cytoophidium in human hepatocellular carcinoma. PMID: 29097181
  2. The filament structures elucidate allosteric mechanisms of assembly and regulation that rely on a conserved conformational equilibrium. The findings may provide a mechanism for increasing human CTP synthase activity in response to metabolic state and challenge the assumption that metabolic filaments are generally storage forms of inactive enzymes. PMID: 28459447
  3. Rods and rings (RR) are protein assemblies composed of CTPS1 and IMPDH2. Glutamine deprivation initiates reversible assembly of mammalian rods and rings. PMID: 24477477
  4. results highlight a key and specific role of CTPS1 in the immune system by its capacity to sustain the proliferation of activated lymphocytes during the immune response PMID: 24870241
  5. analysis of the kinetic properties of hCTPS1 and hCTPS2, and determination that Ser(68) is a major site of CTPS2 regulation by phosphorylation PMID: 20739275
  6. CTP synthetase is phosphorylated by protein kinase A PMID: 16179339
  7. Data indicate that protein kinase C phosphorylation at Ser(462) stimulates human CTP synthetase 1 activity, whereas phosphorylation at Thr(455) inhibits activity. PMID: 17463002
  8. analysis of phosphorylation and regulation of human CTPS1 in human cells shows that GSK3 is a novel regulator of CTPS activity PMID: 17681942
  9. studies provide novel information on the potential interacting proteins that may regulate CTPS1 function or intracellular localization PMID: 18600551

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Involvement in disease
Immunodeficiency 24 (IMD24)
Subcellular Location
Cytoplasm, cytosol.
Protein Families
CTP synthase family
Tissue Specificity
Widely expressed.
Database Links

HGNC: 2519

OMIM: 123860

KEGG: hsa:1503

STRING: 9606.ENSP00000361699

UniGene: Hs.473087

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