CUL2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA108225A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:PC-3 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), K562 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A431 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat Small intestine lysate(30µg) All lanes: Cullin 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 87 kDa Observed band size: 87 kDa Exposure time：120s IHC image of CSB-RA108225A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA108225A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q13617

Target Names

CUL2

Alternative Names

Cul2 antibody; CUL 2 antibody; CUL-2 antibody; CUL2 antibody; CUL2_HUMAN antibody; cullin 2 antibody; cullin homolog 2 antibody; Cullin-2 antibody; MGC131970 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human CUL2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13C2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cullin-2 serves as a critical scaffold protein within the E3 ubiquitin ligase complex, orchestrating the targeted degradation of proteins essential for cell cycle regulation, hypoxia response, and tumor suppression. As a core component of the VHL-containing complex, CUL2 plays a pivotal role in regulating HIF-1α stability, making it a protein of significant interest in cancer biology, oxygen sensing research, and studies of the ubiquitin-proteasome pathway.

This recombinant monoclonal antibody, generated from clone 13C2, offers the consistency and reproducibility that demanding research protocols require. Because the antibody sequence is defined and produced recombinantly, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis confirms detection of CUL2 at the expected 87 kDa molecular weight across diverse human cell lines including PC-3, MCF7, K562, HEK293, and A431, with cross-species reactivity verified in mouse brain tissue and rat small intestine lysates. Immunohistochemistry staining in human testis tissue reveals clear localization patterns, while flow cytometry analysis using 786-O cells shows distinct positive signal separation from isotype controls, confirming utility for intracellular protein detection in fixed and permeabilized cells.

Whether investigating ubiquitin ligase complex assembly, exploring hypoxia signaling mechanisms, or characterizing protein degradation pathways, this antibody provides the flexibility to move seamlessly between western blotting, tissue staining, and flow cytometry workflows with validated, optimized protocols already established.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins.

Gene References into Functions

direct evidence that both A3A and HPV16 E7 interact with CUL2, suggesting that the E7-CUL2 complex formed during HPV infection may regulate A3A protein levels in the cell. PMID: 29367246
The crystal structure of a pentameric CRL2(VHL) complex, composed of Cul2, Rbx1, Elongin B, Elongin C, and pVHL reveals hydrophobic contact residues critical for the Cul2 interactions. PMID: 28591624
Results suggest that E7 recruited CUL2, driven by CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates HPV16-induced cervical carcinogenesis. PMID: 27153550
Cullins are a family of NEDD8 targets important in the stabilization and degradation of proteins, such as hypoxia-inducible factor (HIF1A), via Cullin-2. PMID: 25326537
Nevertheless, the identification of the Cul2 box may allow prediction of Cullin specificity for all E4orf6-containing Adenoviridae. PMID: 24453364
a novel link between the oncoprotein PRAME and the conserved EKC complex PMID: 22912744
Study finds that UBXN7 over-expression converts CUL2 to its neddylated form and causes the accumulation of non-ubiquitylated HIF1alpha. PMID: 22537386
The authors applied protein-complex purification strategies and identified PRAME as a substrate recognition subunit of a Cullin2-based E3 ubiquitin ligase. PMID: 21822215
The data suggest that neither HIF1alpha nor CUL2 mutation may play a central role in HIF1alpha activation in gastric, colorectal, breast, lung and hepatocellular carcinomas, and acute leukemias. PMID: 20078552
Cullin selection is determined by specific Elongin C and Skp1 sequences PMID: 15280393
the HPV16 E7-associated cullin 2 ubiquitin ligase complex contributes to aberrant degradation of the pRB tumor suppressor in HPV16 E7-expressing cells. PMID: 17609271
CUL2 is required for normal vasculogenesis, at least in part mediated by its regulation of HIF-mediated transcription. PMID: 18372249

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Protein Families

Cullin family

Database Links

HGNC: 2552

OMIM: 603135

KEGG: hsa:8453

STRING: 9606.ENSP00000444856

UniGene: Hs.82919

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

HIF-1 signaling pathway Ubiquitin mediated proteolysis Virus Infection

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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