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CUSABIO's strategy for developing a recombinant monoclonal antibody targeting EGF began with the immunization of a rabbit using a synthesized peptide derived from human EGF protein. Subsequent steps involved isolating B cells from the immunized rabbit and extracting RNA from these cells. The extracted RNA was reverse-transcribed into cDNA, which was employed as a template for extending EGF antibody genes using degenerate primers. These engineered EGF antibody genes were then incorporated into a plasmid vector and introduced into host cells for expression. The resulting EGF recombinant monoclonal antibody was isolated from the cell culture supernatant via affinity chromatography and assessed for its suitability in ELISA, IHC, and FC applications, demonstrating specific recognition of human EGF protein.
EGF is a versatile and essential growth factor that regulates various cellular processes, including cell proliferation, differentiation, and tissue repair. Its signaling through the EGFR is tightly controlled to ensure proper tissue development and maintenance.
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