Recombinant Human Pro-epidermal growth factor protein(EGF),partial

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Code CSB-AP002581HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0x106 IU/mg.
Target Names EGF
Uniprot No. P01133
Research Area Cancer
Alternative Names Beta urogastrone; beta-urogastrone; EGF; EGF_HUMAN; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone
Species Homo sapiens (Human)
Source E.coli
Expression Region 971-1023aa
Complete Sequence NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Mol. Weight 6.2 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 m filtered 20 mM PB, pH 7.2
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro
Gene References into Functions
  1. Our results show that the chimeric EGFETA toxin is extremely effective against EGFRpositive cancers and raises the potential to further develop this chimera for use in targeting EGFRpositive tumours resistant to monoclonal antibodies. PMID: 30226622
  2. These results highlight the potential role of EGF in promoting hepatocellular carcinoma (HCC) metastasis, demonstrate a novel pathway for regulation of FN expression and provide potential targets for HCC prevention and treatment. PMID: 29315755
  3. The abnormally elevated expression of EGF and TGF-alpha are closely associated with the occurrence and development of chronic pancreatitis and pancreatic cancer PMID: 29125273
  4. ERRa positively regulated the cell proliferation, migration and invasion of colon cancer cells, and the suppression of ERRa completely reduced the EGF treatment-induced proliferation of colon cancer cells. PMID: 30185207
  5. EGF significantly upregulated RFPL3 and hTERT protein levels in the nonsmall cell lung cancer cells. RFPL3 and hTERT proteins upregulation by EGF were attenuated by pretreatment with AG1478 and erlotinib. EGF promoted proliferation and inhibited apoptosis; PD98059 decreased RFPL3 and hTERT protein expression; and RFPL3 overexpression increased the expression of hTERT and related MEKpathway proteins. PMID: 29749533
  6. we have discovered the novel N-72, and it was crucial for EGF-induced migration by targeting MMP2 in Human amnion mesenchymal stem cells (hAMSCs) PMID: 29734654
  7. The spleen can regulate the functions of hematopoietic stem cells in cirrhotic hypersplenism by regulating EGF signaling. PMID: 29721775
  8. After HIP1 expression was blocked by siRNAs, EGFR endocytosis was accelerated and this effect was dependent on the EGF concentration. This endocytosis was colocalized with clathrin expression. These findings indicate that the inhibition of HIP1 can accelerate the endocytosis and degradation of EGFR PMID: 29039605
  9. the present study demonstrated that EGF induced aggressiveness of gastric cancer cells by activating epithelial to mesenchymal transition, which involved the activation of the ERK1/2 pathway and, subsequently, uPAR expression PMID: 28849196
  10. the EGF system is a mechanosensitizer in bone marrow stromal cells. PMID: 28843157
  11. EGF counteracts Tat modulation of human endogenous retroviruses of the W family in astrocytes. PMID: 28474333
  12. FTIR spectra of EGF, unconjugated, post treatment with alpha-lipoic acid, attached to gold nanoparticle, and bound to the bifunctional nanoprobe, showed decreasing disordered structures and turns, and increasing loops, as the synthesis process progressed. There was an overall increase in beta-sheets in final product compared to pure EGF, but this increase was not linear and fluctuated. PMID: 29122663
  13. EGF-mediated lysosome trafficking, protease secretion, and invasion is regulated by the activity of p38 mitogen activated protein kinase (MAPK) and sodium hydrogen exchangers (NHEs). Interestingly, EGF stimulates anterograde lysosome trafficking through a different mechanism than previously reported for HGF, suggesting that there are redundant signaling pathways that control lysosome positioning PMID: 28978320
  14. Although the diabetic chronic wounds microenvironment is hostile for local GFs bioavailability, EGF local infiltration circumvented the limitations of its topical application, thus expanding its therapeutic prospect. Our clinical pharmacovigilance and basic studies attest the significance of the GF local infiltration for chronic wounds healing. PMID: 28904952
  15. these results provide the first evidence for an association between the EGF rs2298999 C/T polymorphism and gout PMID: 27506295
  16. The increased EGFR expression revealed in patients with seborrheic keratomas (SK)and concomitant (type 2 diabetes mellitus (DM2))is caused by insulin resistance and hyperinsulinemia, in which the dysregulation of insulin signal transmission into the cell leads to changes in EGF synthesis and signaling pathway that regulates cell proliferation and growth. PMID: 28791994
  17. novel EGFR-NF-kappaB-FOXC1 signaling axis that is critical for BLBC cell function PMID: 28629477
  18. EGFR pathway gene expression analysis indicated that DeltaNp63 alters EGFR-regulated genes involved in cell adhesion, migration, and angiogenesis. Addition of EGF or neutralizing EGFR antibodies demonstrated that EGFR activation is responsible for DeltaNp63-mediated loss of cellular adhesion PMID: 28349272
  19. EGF up-regulated CCL2 expression in HNSCC cells, which recruited monocytes and turned them into M2-like macrophages, thus forming a positive feedback paracrine loop. PMID: 27888616
  20. this study shows that EGF induces epithelial-mesenchymal transition through phospho-Smad2/3-Snail signaling pathway in breast cancer cells PMID: 27829223
  21. EGF and TNFalpha cooperatively promoted the motility of HCC cells mainly through NF-kappaB/p65 mediated synergistic induction of FN in vitro. These findings highlight the crosstalk between EGF and TNFalpha in promoting HCC, and provide potential targets for HCC prevention and treatment. PMID: 28844984
  22. Data suggest that EGF induces colorectal cancer cells to undergo epithelial-mesenchymal transition, enhances their ability to invade/migrate, and promotes phosphorylation of Ezrin at Tyr353. (EGF = epidermal growth factor) PMID: 28535417
  23. Simulation results indicate that human epidermal growth factor receptor (hEGFR) soluble soluble extracellular domains (sECD):EGF show different dynamic properties between the two pHs, and the complex may have a higher tendency of activation at pH 8.5. PMID: 27179806
  24. EGF and IP-10 were significantly elevated and GRO levels were lower in the tear profile of HIV patients with dry eye disease (DED) compared to immunocompetent patients with DED. PMID: 27585367
  25. Data (including data from studies using transgenic/knockout mice) suggest that surfactant protein A1 (SPA1) interferes with EGF binding to EGFR in pulmonary alveoli cell lines; SPA1 directly binds extracellular domain of EGFR; binding of SPA1 to EGFR appears to be different from binding of SPD to EGFR; binding of SPA1 to EGFR does not suppress EGF-induced phosphorylation of EGFR or cell proliferation. PMID: 28972165
  26. EGF-AREG interplay in airway basal cell stem/progenitor cells is one of the mechanisms that mediates the interconnected pathogenesis of all major smoking-induced lesions in the human airway epithelium. PMID: 27709733
  27. caspase-3 inhibitors also suppressed the attenuation of cell adhesion and phosphorylation of p38 MAPK by EGF-F9. Our data indicated that EGF-F9 activated signals for apoptosis and induced de-adhesion in a caspase-3 dependent manner. PMID: 27129300
  28. evidence that CDK1/2 participate in the regulation of constitutive pre-mRNA splicing by EGF stimulation in MDA-MB-468 cells. PMID: 27109354
  29. The EGF rs4444903 GG genotype is associated with higher susceptibility to HCV-related liver cirrhosis and hepatocellular carcinoma in the Chinese Han population PMID: 28397482
  30. TGF-beta opposes EGF-mediated sensitization to TRAIL-induced caspase-8 activation and apoptosis in non-transformed breast epithelial cells. EGF and TGF-beta finely regulate sensitivity of human breast epithelial cells to TRAIL which may be relevant during morphogenesis. PMID: 27208428
  31. Amplification of the EGFR gene can be maintained and modulated by variation of EGF concentrations in in vitro models of glioblastoma multiforme PMID: 28934307
  32. Our study showed that the EGF61 rs4444903GA genotype had a decreased risk of non-syndromic cleft lip with or without cleft palate. Our data provides further evidence regarding the role of EGF61 variations in the development of non-syndromic cleft lip with or without cleft palate in families of the studied populations PMID: 28906376
  33. Interestingly, EGF rapidly downregulates LINC01089 (here renamed LncRNA Inhibiting Metastasis; LIMT) expression by enhancing histone deacetylation at the respective promoter. PMID: 27485121
  34. EGF-induced, calpain-mediated proteolysis contributes to the rapid destruction of cyclin G2 and that the PEST domain is critical for EGF/calpain actions PMID: 28640887
  35. The salivary levels of EGF are significantly increased during the acute phase of natural rotavirus infection. PMID: 28558652
  36. findings have identified a role for members of these signaling pathways in the regulation of EGF-induced vimentin expression in the MDA-MB-468 breast cancer cell line PMID: 27163529
  37. miR-223 downregulated the local expression of epidermal growth factor (EGF), leading to decreased activation of EGF receptor (EGFR) on target cells and, eventually, dampening a positive EGF-EGFR autocrine/paracrine stimulation loop induced by the post-surgical wound-healing response. PMID: 26876200
  38. EGFR and EGF expression showed no significant difference between placentas from normal pregnancies and those complicated with preeclampsia. PMID: 27657362
  39. Atomistic molecular dynamics simulations show that N-glycosylation of the EGFR extracellular domain plays critical roles in the binding of growth factors, monoclonal antibodies, and the dimeric partners to the monomeric EGFR extracellular domain. PMID: 28486782
  40. CMTM3 decreases EGFR expression, facilitates EGFR degradation, and inhibits the EGF-mediated tumorigenicity of gastric cancer cells by enhancing Rab5 activity. PMID: 27867015
  41. Findings suggest EGF not only promotes the proliferation of adipose stem cells and delays their senescence, but also maintains the differentiation potency of adipose stem cells, which are related to the EGF-induced activation of STAT signal pathway. PMID: 28746211
  42. The results show that the interaction between STS-1 and ShcA is regulated in response to EGF receptor activation. PMID: 28690151
  43. Insulin treatment caused sustained Akt activity, whereas EGF or PDGF-AA promoted transient signaling; PDGF-BB produced sustained responses at higher concentrations.Transient responses to EGF were caused by negative feedback at the receptor level, as a second treatment yielded minimal responses, whereas parallel exposure to IGF-I caused full Akt activation PMID: 27044757
  44. our results indicate that different concentrations of bFGF and EGF supplemented during propagation of neural rosettes are involved in altering the identity of the resultant neural cells. PMID: 27321088
  45. F25P preproinsulin effectively reduced the concentrations of EGF, VEGF, and MMP-9 in the blood of tumor-bearing mice with EGFR-mutant glioblastoma. PMID: 27317648
  46. Conformational stability of the EGFR as influenced by glycosylation, dimerization and EGF hormone binding has been described. PMID: 28019699
  47. Differential expression patterns of EGF, EGFR, and ERBB4 are essential in epithelial restitution and remodeling in nasal epithelium. PMID: 27285994
  48. Phosphorylation and immunohistochemical assays on the EGF receptor in HeLa cells indicate the EGF protein produced in soybean seed is bioactive and comparable to commercially available human EGF. This work demonstrates the feasibility of using soybean seeds as a biofactory to produce therapeutic agents in a soymilk delivery platform. PMID: 27314851
  49. Data suggest that activated platelets release ADAMDEC1, which hydrolyzes pro-EGF (epidermal growth factor) to soluble, active HMW-EGF; proteolytic cleavage of pro-EGF first occurs at the C-terminal arginyl residue of the EGF domain; proteolysis is the regulated, rate-limiting step in generating soluble EGF from activated platelets. PMID: 28455445
  50. Subgroup analysis in a Slovak population by gender showed the genotype EGF G61G and allele G was associated with non significantly increased risk of MDD. PMID: 27755861
  51. EGF G1380A SNP was associated with the susceptibility and prognosis of malignant melanoma. PMID: 28219779
  52. Therefore, EGF is suggested to induce E-cadherin down-regulation at the transcriptional level through the MEK/ERK pathway, which might result in, at least in part, the induction of cellular morphological changes and cell migration in LoVo cells. PMID: 27369075
  53. EGF and insulin receptor tyrosine kinase exemplify how receptor location is coupled to signal transduction. (Review) PMID: 27023845
  54. These results indicate that Kindlin-1 is essential in EGF-induced re-epithelialization in skin wound healing and provide additional rationale for the clinical application of EGF in the treatment of acute wounds. PMID: 28290610
  55. angiopoietin-like 4 plays an important role in the regulation of epidermal growth factor induced cancer metastasis PMID: 27797381
  56. EGF/MCP-1 ratio is independently associated with severity of tubular atrophy and interstitial fibrosis in primary glomerulonephritis, but the ability of EGF/MCP-1 ratio to discriminate moderate to severe IFTA may not be much better than EGF alone. PMID: 27988512
  57. SMOC binds to Pro-EGF, but does not induce Erk phosphorylation via the EGFR. PMID: 27101391
  58. findings indicate that the effects of stimulation with EGF, HGF and TGFbeta on melanoma cell invasiveness could depend on melanoma cell progression stage. PMID: 28076931
  59. Epidermal Growth Factor polymorphisms are associated with increased rate of sustained virological response. PMID: 26854475
  60. Data show that CD133 overexpression resulted in decreased EGF and EGFR expression, increased telomerase reverse transcriptase (TERT) expression, and increased Akt phosphorylation. PMID: 26646272
  61. lP-EGF was internalized by an active and selective mechanism through EGFR without receptor activation. Oxaliplatin LP-EGF decreased IC50 between 48 and 13% in cell EGFR+. PMID: 26892017
  62. This study showed that CTX III inhibitory effect on EGF-evoked invasion of MDA-MB-231 cells is mediated through suppressing EGF/EGFR activation and EMT process. PMID: 26774845
  63. the role of Epidermal Growth Factor (EGF) and Poly-L-Ornithine (PLO) and PDX1 on human amnion epithelial cell differentiation process, is reported. PMID: 27068127
  64. CD24 regulates EGFR signaling by inhibiting EGFR internalization and degradation in a RhoA-dependent manner in gastric cancer cells. PMID: 26830684
  65. EGF induces transient Ras activation and feedback phosphorylation of Sos. PMID: 26861207
  66. This ubiquitin-mediated switch in EGFR trafficking is a uniquely suited solution to suppress spontaneous activation while maintaining responsiveness to EGF. PMID: 26609808
  67. endometrial- and/or conceptus derived EGF effects migration of uterine luminal epithelium. PMID: 26620571
  68. The present study revealed that the EGF A61G genotype may be a novel independent prognostic marker to identify patients at higher risk of occurrence and an unfavourable clinical outcome. PMID: 26625757
  69. co-immobilizing of specific ratios of EGF and VEGF demonstrated a synergistic effect on cell survival compared to each GF captured alone. PMID: 26485166
  70. Results suggest that Cyr61 is induced by EGF through the ERK/CREB signal pathway and that it plays a crucial role in the migration and invasion of anaplastic thyroid cancer cells. PMID: 25773758
  71. Our findings indicate that epidermal growth factor, interleukin-1beta and angiotensin II receptor, type 1 may play important roles in the pathogenesis of endometriosis. PMID: 26366788
  72. that strong FN1 promoter activity drives inappropriate expression of the biologically active portion of EGF PMID: 26691015
  73. Stimulation of bovine oviduct epithelial cell EGFR with EGF (human recombinant EGF) alone or with EGF in postovulatory/follicular phases (not luteal phase) up-regulates phosphorylation of MAPKs; heat blocks effects of EGF on phosphorylation of MAPKs. PMID: 26050642
  74. Salivary secretion of EGF was diminished in Sjogren syndrome patients. PMID: 26399281
  75. EGF-induced sodium influx regulates EGFR trafficking through increased microtubule acetylation. PMID: 26382850
  76. As serum EGF levels appear to be statistically similar in oral lichen planus (OLP) and oral squamous cell carcinoma, it seems that EGF might play a role in the pathogenesis of OLP and its cancerization PMID: 26545991
  77. EGF induces Ca(2+) sensitization in vascular smooth muscle by Rho-kinase-dependent inactivation of MLCP mediated by the EGF receptor/MEK/Erk1/2 pathway PMID: 26004531
  78. Results indicates that EGF +61 A>G polymorphism might increase the risk of gastric cancer, especially in Asians suggesting that EGF +61 A>G polymorphism may play an important role in the development of gastric cancer. [meta-analysis] PMID: 26072068
  79. data suggest the EGF+61 A>G polymorphism was not related with nonsyndromic oral clefts susceptibility in a Brazilian population, but supported the different genetic background between bilateral cleft lip with or without cleft palate and cleft palate only PMID: 26398511
  80. meta-analyses results suggest that the XPD gene, but not the EGF gene, has contributed to cutaneous melanoma susceptibility, and XPD is a possible drug target PMID: 25537294
  81. EFG +61 G/A is not associated with endometriosis susceptibility in Brazilian women. PMID: 25222228
  82. EGF removal or tyrphostin AG1478 treatment of differentiating air-liquid interface cultures from asthmatic children would result in a reduction of epithelial goblet cells and mucus secretion. PMID: 26057128
  83. Regulation of Hyaluronan (HA) Metabolism Mediated by HYBID (Hyaluronan-binding Protein Involved in HA Depolymerization, KIAA1199) and HA Synthases in Growth Factor-stimulated Fibroblasts. PMID: 26518873
  84. modulation of EGF signaling affects in vitro expansion and differentiation of progenitors from embryonic pancreas of both mice and man. PMID: 25925840
  85. AG genotype has a protective effect on OSA patients against severe disease, although EGF 61A/G polymorphisms have no role on the risk of the disease. PMID: 25028172
  86. results suggest that the c-Src, NADPH oxidase, PI3K, and Akt signaling pathways play important roles in EGF-induced NF-kappaB activation and HO-1 expression in HT-29 cells PMID: 25122478
  87. The findings of this study provide evidence that multiple sclerosis spinal cord simultaneously lack Cbl, EGF, and PrPCs. PMID: 25888933
  88. our data indicate that hnRNP acetylation is part of the EGF-inducible response. PMID: 26110767
  89. Human embryo mesenchymal stem cells co-stimulated with EGF transfection and Ca(2+) can differentiate into epithelial-like cells in vitro. PMID: 25339576
  90. We identified a novel EGF variant, which associated with platelet count in our study (P combined = 2.44 x 10(-15)). Our study also replicated 16 genetic associations related to five hematological traits with genome-wide significance PMID: 26064965
  91. results suggest that the positive effect of the combination of EGF and rhBMP-2 is expected to induce the bone formation earlier compared to the single use of rhBMP-2 in vitro and in vivo. PMID: 24764222
  92. We review methods currently available and show the latest results from a new single-molecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) PMID: 26009168
  93. There was evidence for the involvement of PDGF, IGF-1, and EGF in primary pulmonary hypoplasia. PDGF deficiency plays a certain role in secondary pulmonary hypoplasia PMID: 26841646
  94. results define new mechanistic insights for EGF-mediated invasion and novel actions of SK2 PMID: 26209696
  95. MicroRNAs are part of the regulatory network that controls EGF induced apoptosis, including elements of the JAK/STAT pathway, in A431 cells PMID: 25781916
  96. EGF induces microRNAs that target suppressors of cell migration: miR-15b targets MTSS1 in breast cancer. PMID: 25783158
  97. Our study shows that EGF +61A/G polymorphism is significantly associated with the increased hepatocellular carcinoma risk, especially in Asian populations. Further large-scale and well-designed studies are required to confirm this conclusion PMID: 25927412
  98. these results show that head and neck squamous cell cancer stem cells exhibit plasticity, with the maintenance of the stem cell fraction dependent on the EGF and IGF pathways PMID: 25024430
  99. Results suggest that changes in expression levels of EGF and TGFbeta1 resulting from functional polymorphisms, may contribute to an homeostasis disequilibrium and thus to a higher risk for an earlier disease progression of renal Cell Carcinoma . PMID: 25909813
  100. Wnt5a is a potential suppressor of EMT; there is a novel Arf6/ERK signaling pathway for EGF-regulated Wnt5a expression at transcriptional level of gastric cancer cel PMID: 25779663

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Involvement in disease Hypomagnesemia 4 (HOMG4)
Subcellular Location Membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in kidney, salivary gland, cerebrum and prostate.
Database Links

HGNC: 3229

OMIM: 131530

KEGG: hsa:1950

STRING: 9606.ENSP00000265171

UniGene: Hs.419815

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