Product Details

Uniprot No.

Q92769

Target Names

HDAC2

Alternative Names

Histone deacetylase 2 (HD2) (EC 3.5.1.98), HDAC2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HDAC2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9H4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HDAC2 (Histone deacetylase 2) plays a central role in epigenetic regulation by removing acetyl groups from histone lysine residues, leading to chromatin condensation and transcriptional repression. This enzyme participates in critical cellular processes including cell cycle progression, differentiation, and apoptosis, making it a key target in epigenetics research as well as cardiovascular and stem cell biology. Dysregulation of HDAC2 has been implicated in various pathological conditions, positioning it as both a mechanistic focus and potential therapeutic target.

This recombinant monoclonal antibody, clone 9H4, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Because recombinant production eliminates the batch variability inherent in traditional hybridoma methods, you can expect reliable performance across long-term studies and multi-site collaborations without the need to re-optimize conditions between lots.

The antibody has been validated across multiple applications, providing flexibility for diverse experimental approaches. In Western blot analysis, it detects HDAC2 in MCF-7 and K562 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 60 kDa runs slightly higher than the predicted molecular weights of 52-56 kDa, which is consistent with post-translational modifications such as phosphorylation or sumoylation known to occur on HDAC2. Immunohistochemistry validation demonstrates clear nuclear staining in paraffin-embedded human breast cancer and brain tissue sections, while immunofluorescence studies in HeLa cells confirm nuclear localization with distinct signal separation from DAPI counterstaining.

Whether you are investigating chromatin remodeling mechanisms, exploring HDAC2's role in cardiac development, or characterizing stem cell differentiation pathways, this antibody delivers the specificity and application versatility your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.

Gene References into Functions

Results found that HDAC2 expression is up-regulated in patients with chronic diabetic foot ulcer and in high glucose induced endothelial progenitor cells. PMID: 30063937
Comparative molecular docking studies of the lead RH01652 with class I HDACs (HDAC1, HDAC2, HDAC3, and HDAC8) shows higher binding affinity towards HDAC2. Thus, lead RH01652 could serve as template to design novel and potent inhibitor of HDAC2. PMID: 29932788
human immortalized telencephalic/mesencephalic microglial cells reveal significant upregulation of HDAC2 in the presence of the potent microglial activator lipopolysaccharide PMID: 29803514
we found that HDAC2 was the direct target of miR-31 by binding to 3'-UTR from the results of luciferase reporter assays, qRT-PCR, and western blotting. HDAC2 played an activation role in tumor growth, whose expression is upregulated and inversely associated with miR-31 levels PMID: 29333444
data indicate that the obesity impacts on H4ac levels and that strenuous exercise leads to an enhanced chronic low-grade inflammation profile in obesity via an imbalance on H4ac/HDAC2. PMID: 29142617
Knockdown of HDAC2 completely mimicked the effects of 1,25(OH)2D3 on PTEN gene expression. PMID: 28737824
Muscle biopsies of chronic obstructive pulmonary disease significantly correlated with HDAC2 decrease compared to controls. PMID: 28526090
With tissue microarrays of hepatocellular carcinoma (HCC) patients, we determined the prognostic values of the core genes in the network and found that RAD21, CDK1, and HDAC2 expression levels were negatively associated with overall survival for HCC patients. PMID: 28434945
Combined treatment with the histone deacetylase inhibitors (HDACi) suberoylanilide hydroxamic acid plus 5-fluorouracil (5-FU) and oxaliplatin (Oxa) reduced the level of HDAC2 expression in HT-29 cells. PMID: 27283986
miR-223 controls the expression of CX3CL1 by targeting HDAC2 in chronic obstructive pulmonary disease patients and mouse models of the disease. PMID: 26864305
Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells. PMID: 28985013
study defines two classes of HDAC2 targets in human cells, with a dependence of HDAC1 on HDAC2 at one class of targets, and distinguishes unique functions for HDAC2 PMID: 28982113
This study demonstrated that the Expression of HDAC2 Transcript Is Reduced in Dorsolateral Prefrontal Cortex of Patients with Schizophrenia. PMID: 27959513
The findings suggest that miR-455-3p plays a critical role during chondrogenesis by directly targeting HDAC2/8 and promoting histone H3 acetylation. PMID: 27638301
report the interaction between CFTR and HDAC2, and its involvement in the development of Ph+ leukemia PMID: 28235656
USP4 inhibits p53 and NF-kappaB through deubiquitinating and stabilizing HDAC2 PMID: 26411366
HDAC2 controls ciliogenesis independently of Kras, which facilitates Aurora A expression. These studies suggest that HDAC2 is a novel regulator of primary cilium formation in PDAC cells. PMID: 28028031
the current findings implicate the HDAC2/miR-101/AMPK pathway as a critical mediator of AD pathogenesis. These studies also highlight the importance of epigenetics in AD and provide novel therapeutic targets. PMID: 28202389
HDAC2 upregulation is associated with hepatocellular carcinoma. PMID: 27342975
Suggest that HDAC2 can trigger migration and invasion of non-small cell lung carcinoma cells by activating NF-kappaB to up-regulate fibronectin expression. PMID: 27665474
KLF4 acts as a tumor suppressor or oncogene to activate or repress target gene transcription depending on its acetylation status, which is regulated by p21 and CK2 interaction-mediated HDAC2 phosphorylation PMID: 26729194
Decreased HDAC2 expression is associated with cisplatin resistance in ovarian cancer. PMID: 26683361
In cigarette smoke extract-exposed airway epithelial cells and macrophages, HDAC2 is excessively ubiquitinated and degraded in the proteasome attributed to low expression of USP17. PMID: 26617781
The results imply that HDAC2 is involved in the transcriptional regulation of human odontoblasts in vivo. PMID: 22297573
Endothelial-Mesenchymal Transition is initiated by recruitment of aberrantly phosphorylated DNMT1 to the RASAL1 CpG island promoter by HDAC2, causing aberrant promoter methylation and transcriptional suppression. PMID: 26815200
Selective inhibition of HDAC2 in lung tumor cell causes survivin downregulation through activation of p53, which is mediated by downregulation of Mdm2. PMID: 25605253
our study revealed that HDAC2 is overexpressed in colorectal cancer (CRC) cells; its knockdown can increase the sensitivity of CRC cells to doxorubicin via upregulation of ABCB1 PMID: 26846508
Pancreatic adenocarcinoma patients with enhanced HDAC-1 and -6 expression showed significantly longer survival times compared to those with low expression, while a borderline association concerning HDAC-2 expression was noted. PMID: 26502922
Lymphocyte senescence in COPD is associated with loss of HDAC2 in CD28nullCD8+ T and NKT-like cells. PMID: 26498345
HDAC2 may not confer susceptibility to Schizophrenia in Han Chinese. PMID: 26063464
In endothelial dysfunction, HDAC2 levels were reciprocally regulated by ectopic expression of NEDD8 and the de-NEDDylating enzyme SENP8. PMID: 25655932
Aberrant overexpression of HDACs in basal cells of IPF lungs may contribute to the bronchiolisation process in this disease. Similarly, generation and apoptosis resistance of IPF fibroblasts are mediated by enhanced activity of HDAC enzymes. PMID: 26359372
HDAC2 sumoylation is important for NF-kappaB-dependent gene expression and for the resistance of cell against stress PMID: 25704882
HDAC2 inhibition reduces proliferation and induces apoptosis via the caspase-dependent pathway in human glioma cell lines, possibly by activating the p53 signaling pathway. PMID: 25523932
Data indicate that that hstone deacetylase 2 (HDAC2) has a specific role in leukemogenesis. PMID: 25473896
HDAC3 is an essential target to disrupt HIV-1 latency, and inhibition of HDAC2 may also contribute to the effort to purge and eradicate latent HIV-1 infection. PMID: 25136952
BRG1/HDAC2 and beta-catenin constitute a manipulative apparatus at the transcription start site to play opposite but complementary roles in regulating hTERT expression. PMID: 25486475
HDAC2 plays a central role in coupling lysine acetylation to synaptic plasticity and mediates many of the effects of HDAC inhibition in cognition and disease. PMID: 25492968
Data suggest that p15RS (p15INK4b-related sequence) acts as an intrinsic transcriptional repressor for Wnt/beta-catenin-mediated gene transcription through recruiting HDAC2 histone deacetylase. PMID: 25697359
Simultaneous loss of Hdac1 and Hdac2 resulted in loss of hematopoietic stem cells. PMID: 24763403
our data suggested that cPA may have beneficial effects in inflammation-related cardiovascular disease by controlling HDAC2 regulation PMID: 25013374
HDAC2 silencing in HCC cells also strongly inhibited PPARgamma signaling. PMID: 24958469
inhibitory of cell growth by melittin might be led by HDAC2-mediated PTEN upregulation, Akt inactivation, and inhibition of the PI3K/Akt signaling pathways PMID: 24788349
Osteocalcin levels were decreased, an effect induced at the transcriptional level, and were strongly correlated with inhibition of HDAC2. PMID: 24105979
these results indicated that the potential of specific inhibition of HDAC2 by small molecular chemicals may lead to future therapeutic agents in human renal cancer treatment. PMID: 24390319
The proliferation inhibition and cell cycle arrest mediated by downregulated HDAC2 expression may be tightly associated with the decrease of cyclin D1, cyclin E, and cdk2 proteins expression. PMID: 24965412
PELP1 regulates tumor metastasis by controlling the expression and functions of the tumor metastasis suppressors miR-200a and miR-141 PMID: 23975430
HDAC2 is a critical regulator of Arg2 expression and thereby endothelial nitric oxide and endothelial function. PMID: 24833798
Data indicate prominent changes in urothelial cancer cell lines (UCC) were HDAC2 and/or HDAC8 up-regulation. PMID: 22944197
our study has found no association between HDAC2/HDAC3 gene polymorphisms and schizophrenia in the Chinese Han population PMID: 23857786

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Histone deacetylase family, HD type 1 subfamily

Tissue Specificity

Widely expressed; lower levels in brain and lung.

Database Links

HGNC: 4853

OMIM: 605164

KEGG: hsa:3066

STRING: 9606.ENSP00000430432

UniGene: Hs.3352

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Code	CSB-RA949799A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, K562 whole cell lysate All lanes: HDAC2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 52 kDa Observed band size: 60 kDa IHC image of CSB-RA949799A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA949799A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA949799A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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HDAC2 Recombinant Monoclonal Antibody

Product Details

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Target Background