Code | CSB-RA949799A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
CUSABIO injected a rabbit with a human HDAC2-derived peptide to elicit an immune response. Splenocytes were subsequently isolated from the immunized rabbit, and RNA was extracted from the splenocytes. Reverse transcription converted the extracted RNA into cDNA, which served as a template for extending the HDAC2 antibody gene using degenerate primers. Following purification, the HDAC2 antibody gene fragments were cloned into an expression vector and transfected into a host system for antibody production. The resulting HDAC2 recombinant monoclonal antibodies were purified using affinity chromatography from the cell culture supernatant. To confirm the specificity and affinity of the HDAC2 recombinant monoclonal antibody, four applications ELISA, WB, IHC, and IF were employed, verifying its selective recognition of the human HDAC2 protein.
There are currently no reviews for this product.