Code | CSB-RA010829A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The production of the HSPA8 recombinant monoclonal antibody involves the application of DNA recombinant technology and in vitro genetic manipulation. To begin, an animal is immunized with a synthesized peptide derived from human HSPA8, resulting in the isolation of B cells. The positive B cells are then screened and the single clone is identified. The light and heavy chains of the HSPA8 antibody are amplified using PCR and inserted into a plasmid vector to create a recombinant vector. This vector is transfected into a host cell line to enable the expression of the antibody. The HSPA8 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. This antibody exhibits high specificity for human HSPA8 protein and is suitable for use in ELISA and WB applications.
The HSPA8 protein, also known as HSC70, is constitutively expressed in cells and plays a critical role in maintaining protein homeostasis. It is involved in the cellular response to stress, such as heat shock, oxidative stress, and DNA damage. Under stress conditions, HSPA8 is upregulated and can help protect cells by preventing protein aggregation and promoting protein refolding or degradation. HSPA8 can interact with other chaperones, co-chaperones, and regulatory factors to form a protein complex that regulates various cellular processes, such as protein trafficking, signaling, and cell cycle progression. Additionally, HSPA8 can interact with signaling proteins, such as BAX and p53, to regulate apoptosis and cell survival.
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