Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-RA225697A0HU |
| Size | US$210 |
| Order now | |
| Image |
|
| Have Questions? | Leave a Message or Start an on-line Chat |
| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:50-1:200 |
To generate a monoclonal antibody against IGFBP2, B cells were obtained from an animal immunized with a synthesized peptide from human IGFBP2 and subsequently fused with myeloma cells to create hybridomas. The variable light (VL) and variable heavy (VH) domains of the IGFBP2 antibody-producing hybridomas were sequenced and used to synthesize the IGFBP2 monoclonal antibody gene. The gene is inserted into a plasmid vector. The vector containing the IGFBP2 monoclonal antibody gene is transfected into cells, and the IGFBP2 recombinant monoclonal antibody is collected and purified from the cell culture supernatant using affinity chromatography. The specificity of the purified antibody was evaluated using ELISA, WB, and IHC assays, and this antibody was shown to specifically recognize human IGFBP2 protein.
The IGFBP2 protein plays a role in regulating cell growth and differentiation. It binds to insulin-like growth factors (IGFs) and modulates their biological effects, including cell proliferation, differentiation, and apoptosis. Additionally, IGFBP2 has been implicated in the regulation of glucose metabolism and the development of certain cancers. It is expressed in various tissues including the liver, brain, and adipose tissue.
There are currently no reviews for this product.
